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Anim Genet ; 32(4): 222-5, 2001 Aug.
Article in English | MEDLINE | ID: mdl-11531703

ABSTRACT

Genetic variability was analysed in two common breeds of pheasant (Phasianus colchicus L. 1758) by means of cross-species amplifications of microsatellite loci: 154 chicken, Gallus gallus and 32 turkey, Meleagris gallopavo, primers were tested for amplification of pheasant DNA. Thirty-six primers (25 specific for chicken and 11 for turkey) amplified pheasant DNA. Fifteen markers yielded specific products and were tested for polymorphism. Eight of them (55%) were polymorphic, with an average polymorphism of two alleles per locus. Specific polymerase chain reaction (PCR) products were sequenced; repeats were found in 11 of the 15 markers, although only two loci showed the same repeat and could be homologous to chicken ones.


Subject(s)
Birds/genetics , Microsatellite Repeats/genetics , Polymorphism, Genetic , Alleles , Animals , Chickens , DNA/chemistry , DNA/genetics , DNA/isolation & purification , Genetic Variation/genetics , Polymerase Chain Reaction/veterinary , Sequence Analysis, DNA , Turkey
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