Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 4 de 4
Filter
Add more filters










Database
Language
Publication year range
1.
Virus Res ; 276: 197823, 2020 01 15.
Article in English | MEDLINE | ID: mdl-31765690

ABSTRACT

Cytoplasmic RNA granules consist of microscopic agglomerates of mRNAs and proteins and occur when the translation is reversibly and temporally halted (stress granules, SGs) or mRNAs are targeted for decapping (processing bodies, PBs). The induction of RNA granules formation by virus infection is a common feature of mammalian cells. However, plant-virus systems still remain poorly characterized. In this work, the SG marker AtUBP1b was expressed in Nicotiana benthamiana plants to decipher how the virus infection of plant cells affects SG dynamics. We found that the hypoxia-induced SG assembly was substantially inhibited in Potato virus X (PVX)-infected cells. Furthermore, we determined that the expression of PVX movement protein TGBp1 by itself, mimics the inhibitory effect of PVX on SG formation under hypoxia. Importantly, overexpression of AtUBP1b showed inhibition of the PVX spreading, whereas the overexpression of the dominant negative AtUBP1brrm enhanced PVX spreding, indicating that AtUBP1b negatively affects PVX infection. Notably, PVX infection did not inhibit the formation of processing bodies (PBs), indicating PVX has distinct effects depending on the type of RNA granule. Our results suggest that SG inhibition could be part of the virus strategy to infect the plant.


Subject(s)
Cytoplasmic Granules/metabolism , Nicotiana/virology , Plant Proteins/metabolism , Potexvirus/genetics , RNA, Viral/metabolism , Anaerobiosis , Plant Proteins/genetics , Potexvirus/physiology , RNA, Viral/genetics , Stress, Physiological , Viral Proteins/genetics , Viral Proteins/metabolism
2.
Plant Cell Environ ; 39(1): 165-73, 2016 Jan.
Article in English | MEDLINE | ID: mdl-26147377

ABSTRACT

INCREASED SIZE EXCLUSION LIMIT 2 (ISE2) encodes a putative DEVH-box RNA helicase originally identified through a genetic screening for Arabidopsis mutants altered in plasmodesmata (PD) aperture. Depletion of ISE2 also affects chloroplasts activity, decreases accumulation of photosynthetic pigments and alters expression of photosynthetic genes. In this work, we show the chloroplast localization of ISE2 and decipher its role in plastidic RNA processing and, consequently, PD function. Group II intron-containing RNAs from chloroplasts exhibit defective splicing in ise2 mutants and ISE2-silenced plants, compromising plastid viability. Furthermore, RNA immunoprecipitation suggests that ISE2 binds in vivo to several splicing-regulated RNAs. Finally, we show that the chloroplast clpr2 mutant (defective in a subunit of a plastidic Clp protease) also exhibits abnormal PD function during embryogenesis, supporting the idea that chloroplast RNA processing is required to regulate cell-cell communication in plants.


Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis/enzymology , Gene Expression Regulation, Plant , Plasmodesmata/metabolism , RNA Helicases/genetics , RNA Splicing , Arabidopsis/genetics , Arabidopsis Proteins/metabolism , Biological Transport , Chloroplasts/enzymology , DEAD-box RNA Helicases/genetics , DEAD-box RNA Helicases/metabolism , Genes, Reporter , Introns/genetics , Mutation , Photosynthesis , Plants, Genetically Modified , RNA Helicases/metabolism , RNA, Chloroplast/genetics , RNA, Chloroplast/metabolism , Seedlings/drug effects , Seedlings/enzymology , Seedlings/genetics , Seedlings/metabolism
3.
J Biotechnol ; 167(4): 412-9, 2013 Sep 20.
Article in English | MEDLINE | ID: mdl-23896218

ABSTRACT

Citrus canker provoked by Xanthomonas axonopodis pv. citri is a bacterial disease causing severe losses in all citrus-producing areas around the world. Xanthomonas infection is considered as an endemic disease in Northeast and Northwest Argentina, affecting as much as 10% of commercial citrus plantations. There is not known natural resistance neither in orange varieties nor in rootstocks used for grafting of commercial cultivars. To introduce resistance to this disease, plants of Pineapple sweet orange were transformed with a genetic construct allowing constitutive accumulation of dermaseptin. In comparison with non-transformed plants, transgenic plants showed symptom reduction levels of up to 50% in in planta assays performed under controlled conditions.


Subject(s)
Amphibian Proteins/genetics , Amphibian Proteins/metabolism , Antimicrobial Cationic Peptides/genetics , Antimicrobial Cationic Peptides/metabolism , Citrus sinensis/genetics , Citrus sinensis/microbiology , Plant Diseases/microbiology , Plant Diseases/prevention & control , Xanthomonas/drug effects , Agrobacterium tumefaciens , Amphibian Proteins/pharmacology , Anti-Bacterial Agents/metabolism , Anti-Bacterial Agents/pharmacology , Antimicrobial Cationic Peptides/pharmacology , Drug Resistance, Bacterial , Gene Expression Regulation, Plant , Open Reading Frames , Plant Leaves/microbiology , Plants, Genetically Modified , Transformation, Genetic
4.
J Biotechnol ; 157(2): 334-43, 2012 Jan 20.
Article in English | MEDLINE | ID: mdl-22115953

ABSTRACT

Solanum tuberosum plants were transformed with three genetic constructions expressing the Nicotiana tabacum AP24 osmotine, Phyllomedusa sauvagii dermaseptin and Gallus gallus lysozyme, and with a double-transgene construction expressing the AP24 and lysozyme sequences. Re-transformation of dermaseptin-transformed plants with the AP24/lysozyme construction allowed selection of plants simultaneously expressing the three transgenes. Potato lines expressing individual transgenes or double- and triple-transgene combinations were assayed for resistance to Erwinia carotovora using whole-plant and tuber infection assays. Resistance levels for both infection tests compared consistently for most potato lines and allowed selection of highly resistant phenotypes. Higher resistance levels were found in lines carrying the dermaseptin and lysozyme sequences, indicating that theses proteins are the major contributors to antibacterial activity. Similar results were obtained in tuber infection tests conducted with Streptomyces scabies. Plant lines showing the higher resistance to bacterial infections were challenged with Phytophthora infestans, Rhizoctonia solani and Fusarium solani. Considerable levels of resistance to each of these pathogens were evidenced employing semi-quantitative tests based in detached-leaf inoculation, fungal growth inhibition and in vitro plant inoculation. On the basis of these results, we propose that stacking of these transgenes is a promising approach to achieve resistance to both bacterial and fungal pathogens.


Subject(s)
Amphibian Proteins/genetics , Antimicrobial Cationic Peptides/genetics , Plant Proteins/genetics , Plants, Genetically Modified/genetics , Solanum tuberosum/genetics , Amphibian Proteins/metabolism , Animals , Antimicrobial Cationic Peptides/metabolism , Bacteria/genetics , Chickens/genetics , Fungi/genetics , Gene Expression Regulation, Plant , Muramidase/genetics , Muramidase/metabolism , Pectobacterium carotovorum/pathogenicity , Plant Diseases/genetics , Plant Diseases/microbiology , Plant Proteins/metabolism , Solanum tuberosum/microbiology , Nicotiana/genetics
SELECTION OF CITATIONS
SEARCH DETAIL
...