[Determination of 2.4-dinitrophenol, its basic metabolite and 4-oxide diphenyl in biological materials]. / Opredelenie 2,4-dinitrofenola, ego osnovnogo metabolita i 4-oksidifenila v biologicheskom materiale.

A possibility was considered to isolate, by using dimethylformamide, 2.4-dinitrophenol, 2-amino-4-nitrophenol and 4-oxid-diphenyl from a biological-origin object. A method of extraction with subsequent chromatography in thin silicon-gel layer was used to purify the isolates from biological materials. The designed technique provides for identifying and for quantitatively defining the discussed phenol structures contained in the isolates from the liver tissue of corpses and human blood.

[Extraction of 2-amino-4-nitrophenol and 4-phenylphenol from aqueous solutions]. / Osobennosti ékstragirovaniia 2-amino-4-nitrofenola i 4-fenilfenola iz vodnykh rastvorov.

The authors provide the results of extraction of 2-amino-4-nitrophenol and 4-phenilphenol from aqueous solutions by five organic soluvants. The dependence of the extraction degree on some factors (nature of extragent, pH of aqueous phase medium, extragents saturation with water) was established. Necessary extraction rate for isolation of preset quality of the test substances was calculated.

[The determination of 4-nitrophenols in objects of biological origin]. / Opredelenie 4-nitrofenolov v ob''ektakh biologicheskogo proiskhozhdeniia.

The possibility of isolating 4-nitrophenols from objects of biological origin by acetic anhydride is investigated. Extracts from biological material were purified by extraction followed by silica gel thin layer chromatography. A method for identification and measurement of phenol 4-nitro derivatives in extracts of human cadaveric liver and blood is developed.

[The determination of toxic compounds with a dinitrophenol structure in the forensic chemical study of cadaveric material]. / Opredelenie toksicheskikh soedinenii dinitrofenol'noi struktury pri sudebno-khimicheskom issledovanii trupnogo materiala.

A method for forensic chemical assessment of dinitrophenol toxic compounds has been developed. Acetone was used as the isolating agent. The isolates were purified by extraction followed by adsorption in a column packed with silica gel. The analyzed substances are analyzed by chromogenic reactions, from UV and visible spectra in alkali and DMPA, and by thin-layer and high-pressure chromatography. The method allows the detection of 47-83% dinitrophenols in artificial mixtures with liver tissue (with 50 mg of analyzed substance per 100 g of the object). The detection minimum for this group of compounds is 0.5 to 1.0 mg per 100 g of cadaveric material.

[The determination of toxic compounds with a dinitrophenol structure in biological fluids]. / Opredelenie toksichnykh soedinenii dinitrofenol'noi struktury v biologicheskikh zhidkostiakh.

Describes the specific features of chromatographic behavior of numerous toxic compounds of dinitrophenol structure in a thin layer of silica gel and in columns packed with Silasorb-600 adsorbent for high-pressure liquid chromatography. Demonstrates the possibility of using thin-layer and high-pressure liquid chromatography for identification and measurements of the above compounds in the urine and blood.