ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE: Phaseolus trilobus Ait (Fabaceae) is extensively used by tribal people of Nandurbar district (Maharashtra, India) in the treatment of Jaundice and other liver disorders. AIM: of the present study was to assess the medicinal claim of Phaseolus trilobus as hepatoprotective and antioxidant. MATERIAL AND METHODS: The hepatoprotective activity of methanol and aqueous extract of Phaseolus trilobus was evaluated by bile duct ligation induced liver fibrosis and antioxidant activity was evaluated using in vitro and in vivo antioxidant models viz anti-lipid peroxidation assay, super oxide radical scavenging assay and glutathione estimation in liver. Liver function tests were carried out to detect hepatoprotective activity, which was further supported by histopathological examination. RESULTS: Methanol and aqueous extracts of Phaseolus trilobus reduced elevated level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), bilirubin and hydroxyproline significantly (p<0.01) in bile duct ligated Wistar rats, proving hepatoprotective activity comparable with Silymarin. Both the extracts were found to reduce the elevated levels of serum thiobarbituric acid reactive substance (TBARS) and elevate superoxide scavenging radical activity proving antioxidant activity comparable with ascorbic acid. The reduced level of glutathione was found to be elevated in liver proving antioxidant activity comparable with Silymarin. CONCLUSION: Phaseolus trilobus posses hepatoprotective property and is effective in oxidative stress induced cholestatic hepatic injury.
Subject(s)
Antioxidants/therapeutic use , Liver Cirrhosis, Experimental/prevention & control , Liver/drug effects , Phaseolus/chemistry , Plant Extracts/therapeutic use , Animals , Antioxidants/isolation & purification , Antioxidants/pharmacology , Antioxidants/toxicity , Female , Free Radicals/metabolism , Glutathione/metabolism , Liver/metabolism , Liver/pathology , Liver Cirrhosis, Experimental/metabolism , Liver Cirrhosis, Experimental/pathology , Liver Function Tests , Male , Mice , Plant Extracts/isolation & purification , Plant Extracts/pharmacology , Plant Extracts/toxicity , Rats , Rats, Wistar , Superoxide Dismutase/metabolism , Toxicity Tests, AcuteABSTRACT
The tribals residing in Satpuda hills are illiterate, socio-economically backward and still depend on medicinal plants for healthcare and for treatment of various diseases. The objective of the present study was to investigate ethnomedicinal claims of some distinctive medicinal plants utilized by Pawara tribal in the Satpuda hills. Methodology used to investigate ethnomedicinal claims include seasonwise field visits, collection of information from tribal medicine men (Badwa), patients and elderly person in and around study area. Similar use of plants told by minimum three Badwas were taken into consideration and cross-checked with eight elderly persons having traditional knowledge of plants and two to five patients who received treatment from Badwa. Present investigation revealed that tribals are regularly using 52 species of plants belonging to 36 families to cure diseases like skin disorders, burn, diarrhea, jaundice, mouth ulcer, fever, joint pain, abdominal pain, migraine, menstrual problems, urinary problems, wounds, dog bite, as anthelmintic and abortifacient. The study revealed 14 new ethnomedicinal uses of plants not reported previously in the literature and could help to find out new lead compounds for welfare of mankind under present day patent regime. Medicinal plant knowledge gained from tribals would be very useful for pharmacognosists and pharmacologists.
Subject(s)
Medicine, Traditional , Phytotherapy , Plants, Medicinal , Aged , Ethnobotany , Humans , IndiaABSTRACT
The present study was undertaken to examine the effect of pH and concentration of hydroxypropyl-beta-cyclodextrin on the solubility of carvedilol as it shows pH-dependent solubility. The equilibrium solubility of carvedilol in a series of solutions of varying pH (from 1.2 to 11) was determined and compared with the equilibrium solubility of carvedilol in the presence of 20% hydroxypropyl-beta-cyclodextrin at same pH values. It was observed that solubility of protonated form is more than neutral molecule. Hydroxypropyl-beta-cyclodextrin resulted in increased solubility at all the pH. But inclusion in the cavity of hydroxypropyl-beta-cyclodextrin might depend upon charge state of the molecule. So it can be concluded that solubility of carvedilol, can be increased either by the addition of hydroxypropyl-beta-cyclodextrin or by adding pH lowering agents. But both these methods if are to be used together, pH should be selected carefully.
ABSTRACT
A simple, rapid, and accurate high-performance thin-layer chromatography (HPTLC) method is described for the simultaneous determination of levofloxacin hemihydrate and ornidazole in tablet dosage form. The method is based on the HPTLC separation of the two drugs followed by densitometric measurements of their spots at 298 nm. The separation is carried out on Merck TLC aluminium sheets of silica gel 60 F254 using n-butanol-methanol-ammonia (5:1:1.5, v/v/v) as mobile phase. The linearity is found to be in the range of 50-250 and 100-500 ng/spot for levofloxacin hemihydrate and ornidazole, respectively. The method is successively applied to pharmaceutical formulation because no chromatographic interferences from the tablet excipients are found. The suitability of this HPTLC method for the quantitative determination of the compounds is proved by validation in accordance with the requirements laid down by International Conference on Harmonization (ICH) guidelines.
