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Jpn J Cancer Res ; 93(6): 605-9, 2002 Jun.
Article in English | MEDLINE | ID: mdl-12079507

ABSTRACT

We recently identified TSLC1, a tumor suppressor gene in human lung cancer. Gene silencing by promoter methylation has been observed frequently in adenocarcinoma of the lung, liver, and pancreas. Here, we demonstrate that TSLC1 expression is also absent or markedly reduced in 3 of 4 prostate cancer cell lines. Promoter sequences of TSLC1 were heavily methylated in PPC-1 cells that lacked TSLC1 expression, supporting the idea that promoter methylation is strongly correlated with complete loss of gene expression. Promoter sequences of TSLC1 were also methylated significantly in 7 of 22 (32%) primary prostate cancers. Hypermethylation of the promoter occurred not only in advanced tumors, but also in relatively early-stage tumors. Restoration of TSLC1 expression substantially suppressed tumor formation of PPC-1 cells in nude mice. These findings indicate that alteration of TSLC1 is involved in prostate cancer.


Subject(s)
Immunoglobulins , Membrane Proteins , Promoter Regions, Genetic , Prostatic Neoplasms/genetics , Proteins/genetics , Animals , Base Sequence , Blotting, Northern , Cell Adhesion Molecule-1 , Cell Adhesion Molecules , CpG Islands , DNA Methylation , Gene Silencing , Humans , Male , Mice , Mice, Nude , Molecular Sequence Data , Prostatic Neoplasms/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Sulfites/pharmacology , Time Factors , Tumor Suppressor Proteins
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