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Development and application of a Saccharomyces cerevisiae-expressed nucleocapsid protein-based enzyme-linked immunosorbent assay for detection of antibodies against infectious bronchitis virus.
Gibertoni, Aliandra M; Montassier, Maria de Fátima S; Sena, Janete A D; Givisiez, Patrícia E N; Furuyama, Cibele R A G; Montassier, Hélio J.
J Clin Microbiol ; 43(4): 1982-4, 2005 Apr.
Article in English | MEDLINE | ID: mdl-15815038

ABSTRACT

A Saccharomyces cerevisiae-expressed nucleocapsid (N) polypeptide of the M41 strain of infectious bronchitis virus (IBV) was used as antigen in a recombinant yeast-expressed N protein-based enzyme-linked immunosorbent assay (Y-N-ELISA). The Y-N-ELISA was rapid, sensitive, and specific for detecting chicken serum antibodies to IBV, and it compared favorably with a commercial ELISA.


Subject(s)
Antibodies, Viral/blood , Coronavirus Infections/veterinary , Infectious bronchitis virus/immunology , Nucleocapsid Proteins/immunology , Poultry Diseases/diagnosis , Saccharomyces cerevisiae/metabolism , Animals , Chickens , Coronavirus Infections/diagnosis , Coronavirus Infections/virology , Enzyme-Linked Immunosorbent Assay , Nucleocapsid Proteins/metabolism , Poultry Diseases/virology , Saccharomyces cerevisiae/genetics , Sensitivity and Specificity , Time Factors
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