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1.
Auris Nasus Larynx ; 39(2): 216-9, 2012 Apr.
Article in English | MEDLINE | ID: mdl-21602005

ABSTRACT

AIMS: We report the case of a 58-year-old female patient who consulted our Department complaining of positional vertigo and showing spontaneous upbeat nystagmus (UBN) in darkness. METHOD: We analyzed her UBN three-dimensionally. The MRI scan revealed the astrocytoma in the left cerebellum involving the cerebellar vermis. RESULT: Three-dimensional analysis showed a spontaneous UBN rotating around the intra-aural axis in the pitch plane. CONCLUSION: Since the cerebellar vermis is known to plays an inhibitory role on the central vertical vestibule-ocular reflex (VOR), the present results suggest that the spontaneous UBN in darkness observed in this patient was induced by an imbalance of central vertical VOR tone.


Subject(s)
Astrocytoma/physiopathology , Cerebellar Neoplasms/physiopathology , Imaging, Three-Dimensional , Nystagmus, Pathologic/physiopathology , Videotape Recording , Electronystagmography , Female , Humans , Magnetic Resonance Imaging , Middle Aged , Reflex, Vestibulo-Ocular/physiology , Vestibular Function Tests
2.
Article in English | MEDLINE | ID: mdl-16374062

ABSTRACT

Although the bases for both the sporadic and inherited forms of Ménière's disease (MD) remain undefined, it is likely to be multifactorial, one of the factors being a genetic predisposition. Recently, genetic association studies on complex diseases have become very popular and most of them are case-control studies using single nucleotide polymorphisms (SNPs) as markers. Mutations/polymorphisms in KCNE potassium channel genes might play a causative role in MD, because KCNE potassium channels have been suggested to be present and active in transmembrane ion and water transports in the inner ear. In the present study, to identify MD susceptibility genes, we have conducted a genetic association study with optimized sampling, optimized phenotyping/genotyping, and a selection of KCNE genes as the candidate genes. The SNPs analyses identified 112G/A SNP in the KCNE1 gene and 198T/C SNP in the KCNE3 gene in 63 definite MD cases as well as 205 and 237 non-MD control subjects. For both KCNE1 and KCNE3 genes, a significant difference in frequency of each SNP was confirmed between MD cases and non-MD control subjects. The result indicates that 112G/A SNP in the KCNE1 gene and 198T/C SNP in the KCNE3 gene could determine an increased susceptibility to develop MD.


Subject(s)
Meniere Disease/genetics , Polymorphism, Single Nucleotide/genetics , Potassium Channels, Voltage-Gated/genetics , Adult , Case-Control Studies , Female , Gene Frequency/genetics , Genetic Predisposition to Disease/genetics , Heterozygote , Homozygote , Humans , Male , Meniere Disease/pathology , Mutation , Potassium/metabolism
3.
Acta Otolaryngol Suppl ; (553): 13-8, 2004 Aug.
Article in English | MEDLINE | ID: mdl-15277029

ABSTRACT

The aquaporins (AQPs) are a family of small transmembrane water transporters. It has recently been revealed that they play a role in regulating homeostasis in the inner ear fluids. Steroid therapy is usually administered to patients with inner ear disorders; however, the mechanism of steroid effects has not been clearly determined. To elucidate the points of action of steroids in the inner ear, we recently examined the distributions of AQP isoform mRNAs in the rat inner ear and identified AQP1-6 mRNAs in the rat cochlea and AQP1, 3, 4, 5 and 6 mRNAs in the rat endolymphatic sac by means of reverse transcriptase polymerase chain reaction (PCR). In this study, we investigated changes in expression of AQP mRNAs in the rat inner ear after i.p. injections of steroids using real-time quantitative PCR and found that AQP3 mRNA in the endolymphatic sac was significantly upregulated in both dose- and time-dependent manners. This result suggests that steroids may effect water homeostasis in the rat inner ear via AQPs.


Subject(s)
Aquaporins/metabolism , Ear, Inner/metabolism , Steroids/pharmacology , Animals , Aquaporins/drug effects , Aquaporins/genetics , Cochlea/drug effects , Cochlea/metabolism , Dexamethasone/administration & dosage , Dexamethasone/pharmacology , Dose-Response Relationship, Drug , Ear, Inner/drug effects , Ear, Inner/physiology , Electrophysiology , Endolymphatic Sac/drug effects , Endolymphatic Sac/metabolism , Gene Expression , Glucocorticoids/administration & dosage , Glucocorticoids/pharmacology , Injections, Intraperitoneal , Male , RNA, Messenger/metabolism , Rats , Rats, Wistar , Reverse Transcriptase Polymerase Chain Reaction , Steroids/administration & dosage
4.
Acta Otolaryngol ; 122(6): 600-6, 2002 Sep.
Article in English | MEDLINE | ID: mdl-12403121

ABSTRACT

Although steroid treatment is generally administered for patients with inner ear disorders, including Meniere's disease, the mechanism via which steroids exert their effects remains to be clarified. The aquaporins (AQPs) are a family of small transmembrane water transporters, and it has recently been revealed that they play a role in regulating homeostasis in the inner ear fluids. In order to elucidate the action points of steroids in the inner ear, we firstly identified AQPI, 2, 3, 4, 5 and 6 mRNAs in the rat cochlea and AQP1, 3, 4, 5 and 6 in the rat endolymphatic sac by means of reverse transcription-polymerase chain reaction. Subsequently, we found that intratympanic injections of steroids upregulated AQPI mRNA of the rat cochlea in a dose-dependent manner. These results suggest that steroids may affect water homeostasis in the rat inner ear mainly via AQP1.


Subject(s)
Aquaporins/metabolism , Cochlea/metabolism , Dexamethasone/pharmacology , Endolymphatic Sac/metabolism , Glucocorticoids/pharmacology , Animals , Aquaporins/genetics , Cochlea/drug effects , Cochlea/pathology , Dexamethasone/administration & dosage , Endolymphatic Sac/drug effects , Endolymphatic Sac/pathology , Gene Expression , Glucocorticoids/administration & dosage , Injections , Male , RNA, Messenger/metabolism , Rats , Rats, Wistar , Reverse Transcriptase Polymerase Chain Reaction , Tympanic Membrane
5.
J Neurochem ; 81(6): 1308-17, 2002 Jun.
Article in English | MEDLINE | ID: mdl-12068078

ABSTRACT

In order to investigate the mechanisms responsible for adaptation to altered gravity, we assessed the changes in mRNA expression of glutamate receptors in vestibular ganglion cells, medial vestibular nucleus, spinal vestibular nucleus/lateral vestibular nucleus, cerebellar flocculus, and uvula/nodulus from rats exposed to hypergravity for 2 h to 1 week using real-time quantitative RT-PCR methods. The mRNA expression of GluR2 and NR1 receptors in the uvula/nodulus and NR1 receptors in the medial vestibular nucleus increased in animals exposed to 2 h of hypergravity, and it decreased gradually to the control level. The mRNA expression of GluR2 receptors in vestibular ganglion cells decreased in animals exposed to 1 week of hypergravity. Neither the metabotropic glutamate receptor 1 nor delta2 glutamate receptor in flocculus and uvula/nodulus was affected by a hypergravity load for 2 h to 1 week. It is suggested that the animals adapted to the hypergravity by enhancing the cerebellar inhibition of the vestibular nucleus neurons through activation of the NR1 and GluR2 receptors on the Purkinje cells in uvula/nodulus especially at the early phase following hypergravity. In the later phase following hypergravity, the animals adapted to the hypergravity by reducing the neurotransmission between the vestibular hair cells and the primary vestibular neurons via down-regulation of the postsynaptic GluR2 receptors in the vestibular periphery.


Subject(s)
Cerebellum/metabolism , Hair Cells, Vestibular/metabolism , RNA, Messenger/metabolism , Receptors, Glutamate/genetics , Vestibular Nuclei/metabolism , Animals , Male , Rats , Rats, Wistar , Reverse Transcriptase Polymerase Chain Reaction
6.
Laryngoscope ; 112(2): 255-61, 2002 Feb.
Article in English | MEDLINE | ID: mdl-11889380

ABSTRACT

OBJECTIVES: Recently, we identified three novel mutations of the GJB2 gene in Japanese families with autosomal-recessive non-syndromic deafness.1 Seven of 11 mutated chromosomes (63.6%) contained a 233delC allele, suggesting that the 233delC mutation is the most common mutation of the GJB2 gene in the Japanese population. After it was recognized that cochlear implantation (CI) is of benefit to children with prelingual deafness, we have had a number of prelingual pediatric CI patients. Because children carrying the homozygous 233delC mutation show bilateral prelingual profound deafness, they could be enrolled in the CI program at Osaka University Graduate School of Medicine. The purposes of this study were 1) to analyze the occurrence of the GJB2 mutations in our 15 prelingual pediatric CI patients in whom the cause of non-syndromic deafness was unknown, and 2) to evaluate the auditory function and postoperative speech perception with CI of those GJB2-related deaf subjects. STUDY DESIGN: Retrospective analysis. METHODS: Mutation analysis of the GJB2 gene by direct sequencing was performed with genomic DNA from 15 children born profoundly deaf as a result of unknown causes and implanted with CI. Intraoperative electrically evoked auditory brainstem response (EABR) and intra-/postoperative EAP were measured. The speech perception was evaluated with Infants and Toddlers Meaningful Auditory Integration Scale (IT-MAIS). RESULTS AND CONCLUSIONS: We identified 4 CI patients (26.7%) out of 15 children carrying the homozygous 233delC mutation. Intra- and postoperative evaluation of the auditory system revealed almost intact cochlear and retrocochlear auditory function in these 4 patients. Postoperative auditory testing indicates that their speech perception had become significantly higher in comparison with that of other prelingual CI patients. These results suggest that prelingual deaf children carrying the homozygous 233delC mutation of the GJB2 gene can benefit from CI.


Subject(s)
Cochlear Implantation/methods , Connexins/genetics , Deafness/genetics , Deafness/surgery , Point Mutation , Audiometry , Base Sequence , Child, Preschool , Connexin 26 , Deafness/epidemiology , Female , Follow-Up Studies , Humans , Japan/epidemiology , Language Development , Male , Molecular Sequence Data , Polymerase Chain Reaction , Prevalence , Retrospective Studies , Risk Assessment , Speech Perception , Treatment Outcome
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