Molecular dynamics model for the antibactericity of textured surfaces.

An original model has been developed for the initial stage of bacterial adhesion on textured surfaces. Based on molecular dynamics, the model describes contact between individual bacterial cells in a planktonic state and a surface, accounting for both the mechanical properties of the cells and the physico-chemical mechanisms governing interaction with the substrate. Feasibility of the model is assessed via comparison with experimental results of bacterial growth on stainless steel substrates textured with ultrashort laser pulses. Simulations are performed for two different bacterial species, Staphylococcus aureus and Escherichia coli, on two distinct surface types characterised by elongated ripples and isolated nanopillars, respectively. Calculated results are in agreement with experiment outcomes and highlight the role of mechanical stresses within the cell wall due to deformation upon interaction with the substrate, creating unfavourable conditions for bacteria during the initial phases of adhesion. Furthermore, the flexibility of the model provides insight into the intricate interplay between topography and the physico-chemical properties of the substrate, pointing to a unified picture of the mechanisms underlying bacterial affinity to a textured surface.

Ion microscopy based on laser-cooled cesium atoms.

We demonstrate a prototype of a Focused Ion Beam machine based on the ionization of a laser-cooled cesium beam and adapted for imaging and modifying different surfaces in the few-tens nanometer range. Efficient atomic ionization is obtained by laser promoting ground-state atoms into a target excited Rydberg state, then field-ionizing them in an electric field gradient. The method allows obtaining ion currents up to 130pA. Comparison with the standard direct photo-ionization of the atomic beam shows, in our conditions, a 40-times larger ion yield. Preliminary imaging results at ion energies in the 1-5keV range are obtained with a resolution around 40nm, in the present version of the prototype. Our ion beam is expected to be extremely monochromatic, with an energy spread of the order of the eV, offering great prospects for lithography, imaging and surface analysis.