ABSTRACT
Traceability of patients who are candidates for Hematopoietic cell transplant (HCT) is crucial to ensure HCT program quality. Continuous knowledge of both a detailed registry from a HCT program and final exclusion causes can contribute to promoting a real-life vision and optimizing patient and donor selection. We analyzed epidemiological data reported in a 4 year-monocentric prospective registry, which included all patients presented as candidates for autologous (Auto) and/or allogeneic (Allo) HCT. A total of 543 patients were considered for HCT: 252 (42.4%) for Allo and 291 (57.6%) for Auto. A total of 98 (38.9%) patients were excluded from AlloHCT due to basal disease progression more commonly (18.2%). Seventy-six (30.2%) patients had an HLA identical sibling, whereas 147 (58.3%) patients had only Haplo. UD research was performed in 106 (42%) cases, significantly more often in myeloid than lymphoid malignancies (57% vs 28.7%, p < 0.001) but 61.3% were finally canceled, due to donor or disease causes in 72.4%. With respect to Auto candidates, a total of 60 (20.6%) patients were finally excluded; progression was the most common cause (12%). Currently, Haplo is the most frequent donor type. The high cancellation rate of UD research should be revised to optimize further donor algorithms.
Subject(s)
Hematopoietic Stem Cell Transplantation , Donor Selection , Hematopoietic Stem Cell Transplantation/methods , Humans , Registries , Transplantation Conditioning , Transplantation, AutologousABSTRACT
The permeability to cephaloridine was studied in five Haemophilus influenzae strains (four non-typable and one type b) using the Zimmermann and Rosselet method. The beta-lactamase activity was due to a plasmid-encoded TEM-1 enzyme. High permeability coefficients were measured in all strains examined. No great differences in permeability coefficients were found, even between strains with marked differences in OMP electrophoretic profiles.
Subject(s)
Cephaloridine/pharmacokinetics , Haemophilus influenzae/metabolism , Bacterial Typing Techniques , Haemophilus influenzae/classification , Haemophilus influenzae/ultrastructure , Membranes/metabolism , Microbial Sensitivity Tests , Permeability , Species Specificity , beta-Lactamases/metabolismABSTRACT
The outer membrane proteins (OMPs) of several strains of Serratia marcescens have been studied by sodium dodecyl sulphate - urea - polyacrylamide gel electrophoresis. Four major OMPs, named Omp1, Omp2, Omp3, and OmpA (42, 40, 39, and 37 kDa, respectively), have been visualized. The relative proportions of Omp2 and Omp3 depend on cultural conditions (temperature of incubation, osmolarity, and nutrient availability).
Subject(s)
Bacterial Outer Membrane Proteins/chemistry , Serratia marcescens/chemistry , Bacterial Outer Membrane Proteins/analysis , Electrophoresis, Polyacrylamide Gel , Serratia marcescens/ultrastructureABSTRACT
Penicillin-resistant (penr) clinical isolates of Neisseria meningitidis, which do not produce beta-lactamase, were first identified in Spain in 1985; the frequency of their recovery, which has been increasing in the past few years, reached 20% in 1989. Serogrouping, determination of serotypes and subtypes, and multilocus enzyme electrophoresis of the penr strains showed an extensive diversity. Resistance is due, at least in part, to a decreased affinity of penicillin-binding protein (PBP) 2 for penicillin. Similar low-affinity forms of PBP 2 are also found in penr isolates of Neisseria lactamica, Neisseria polysaccharea, and Neisseria gonorrhoeae. Genetic transformation of an N. meningitidis type strain to low-level penicillin resistance with DNA from resistant meningococci and other Neisseria species resulted in transformants that possessed low-affinity forms of PBP 2. These altered forms of PBP 2 have been shown to arise from recombinational events that replace parts of the PBP 2 gene with the corresponding regions from the PBP 2 genes of commensal Neisseria species.
Subject(s)
Bacterial Proteins , Carrier Proteins , Meningitis, Meningococcal/epidemiology , Meningococcal Infections/epidemiology , Muramoylpentapeptide Carboxypeptidase , Neisseria meningitidis/drug effects , Penicillin Resistance/genetics , Hexosyltransferases/genetics , Hexosyltransferases/metabolism , Humans , Meningitis, Meningococcal/microbiology , Meningococcal Infections/microbiology , Multienzyme Complexes/genetics , Multienzyme Complexes/metabolism , Neisseria meningitidis/classification , Neisseria meningitidis/genetics , Penicillin-Binding Proteins , Peptidyl Transferases/genetics , Peptidyl Transferases/metabolism , Spain/epidemiology , beta-Lactamases/biosynthesis , beta-Lactamases/geneticsABSTRACT
During batch aerobic submerged fermentation, the exopolysaccharide synthesis by Pseudomonas sp. strain EPS-5028 occurred in growth- and non-growth-linked processes. Polysaccharide formation increased when the pH was controlled at 7 during fermentation. Exopolysaccharide production depended on the phosphate content of the medium. The polymer exhibited a pseudoplastic nature, had good thermostability, and was affected neither by pH nor by high concentrations of salt.
