Entre la incertidumbre de la liberación y otras formas de uso de la fauna silvestre / Between the uncertainty of release and other forms of wildlife use

RESUMEN Con el proyecto que fundamenta este artículo se desarrolló un análisis detallado del destino final de animales silvestres por parte la autoridad ambiental, con enfoque en los procesos de liberación en los departamentos amazónicos del Amazonas, Caquetá y Putumayo, entre 2019 y 2021, para proponer alternativas efectivas de abordaje y manejo de la situación. Los datos fueron directamente requeridos a la Corporación para el Desarrollo Sostenible del Sur de la Amazonía (Corpoamazonía), y a partir de estos se desarrolló manejo estadístico para posteriormente interpretar los resultados obtenidos a la luz de la literatura académica publicada sobre el tema y la normatividad vigente relacionada. Como producto de la investigación, se identifica que la principal proveniencia de especímenes se establece en la entrega voluntaria (65,59%), seguido del rescate o traslado (27,87%), y decomiso, incautación, aprehensión o medida preventiva (3,96%). Como su principal destino final se identificó la liberación (45,21%). Del total de liberaciones entre 2019 y 2021, solo en 50 (4,09% de los casos), se consignó algún tipo de indicación del lugar o la ubicación específica donde se llevó a cabo, sin mención del procedimiento de caracterización del hábitat o genética del espécimen, o seguimiento posliberación, por lo que resulta necesario potenciar otras formas de uso de la fauna silvestre que permitan reducir el margen de incertidumbre.

ABSTRACT Alongside the project that supports this article, we developed a detailed analysis of the final destination of wild animals by the environmental authority, with a focus on the release processes in the Amazonian departments of Amazonas, Caquetá, and Putumayo, between 2019 to 2021, in order to propose effective alternatives for approaching and managing the situation. The data was directly requested from the Corporation for the Sustainable Development of the South of the Amazon (Corpoamazonía), and they were handled with statistical management to interpret the results obtained according to the academic literature published on the subject and current related regulations. As a result of the investigation, the primary origin of the specimens was identified as voluntary surrender, in 65.59% of the cases; followed by rescue or transfer, in 27.87%; and confiscation, seizure, apprehension, or preventive measure in 3.96%. As their main final destination, release was identified in 45,21% of the cases. Of the total number of releases between 2019 and 2021, only 50 (4.09% of the cases) included some type of indication of the place or specific location where the release took place, with no mention of the habitat or genetic characterization procedure of the specimen, or postrelease follow-up, it is, therefore, necessary to promote other forms of wildlife use that reduce the margin of uncertainty.

Transgenic Citrange troyer rootstocks overexpressing antimicrobial potato Snakin-1 show reduced citrus canker disease symptoms.

Citrus canker is a major disease caused by Xanthomonas citri pv. citri. Snakin-1 is an antimicrobial peptide, which was previously shown to be effective against different bacterial and fungal diseases in potato, wheat and lettuce when expressed in transgenic plants. We generated transgenic Citrange Troyer citrus rootstocks constitutively expressing this peptide and 5 different transgenic lines were challenged against virulent X. citri isolates. Challenge assays conducted in vitro using detached leaves and in planta by infiltration revealed a significant reduction of the number and size of canker lesions in some of the transgenic lines.

Effect of Chronic Consumption of Sweeteners on Microbiota and Immunity in the Small Intestine of Young Mice.

The consumption of sweeteners has increased as a measure to reduce the consumption of calories and thus combat obesity and diabetes. Sweeteners are found in a large number of products, so chronic consumption has been little explored. The objective of the study was to evaluate the effect of chronic sweetener consumption on the microbiota and immunity of the small intestine in young mice. We used 72 CD1 mice of 21 days old, divided into 3 groups: (i) No treatment, (ii) Group A (6 weeks of treatment), and (iii) Group B (12 weeks of treatment). Groups A and B were divided into 4 subgroups: Control (CL), Sucrose (Suc), Splenda® (Spl), and Svetia® (Sv). The following were determined: anthropometric parameters, percentage of lymphocytes of Peyer's patches and lamina propria, IL-6, IL-17, leptin, resistin, C-peptide, and TNF-α. From feces, the microbiota of the small intestine was identified. The BMI was not modified; the mice preferred the consumption of Splenda® and Svetia®. The percentage of CD3+ lymphocytes in Peyer's patches was increased. In the lamina propria, Svetia® increased the percentage of CD3+ lymphocytes, but Splenda® decreases it. The Splenda® and Svetia® subgroups elevate leptin, C-peptide, IL-6, and IL-17, with reduction of resistin. The predominant genus in all groups was Bacillus. The chronic consumption of sweeteners increases the population of lymphocytes in the mucosa of the small intestine. Maybe, Bacillus have the ability to adapt to sweeteners regardless of the origin or nutritional contribution of the same.

Effect of Supplementation with n-3 Fatty Acids Extracted from Microalgae on Inflammation Biomarkers from Two Different Strains of Mice.

BACKGROUND: Diabetes mellitus is considered a chronic noncommunicable disease in which inflammation plays a main role in the progression of the disease and it is known that n-3 fatty acids have anti-inflammatory properties. One of the most recent approaches is the study of the fatty acids of microalgae as a substitute for fish oil and a source rich in fatty acids EPA and DHA. OBJECTIVE: To analyze the effect of supplementation with n-3 fatty acids extracted from microalgae on the inflammatory markers from two different strains of mice. METHODS: Mice of two strains, db/db and CD1, were supplemented with n-3 fatty acids extracted from microalgae in lyophilized form and added to food; the experiment was carried out from week 8 to 16 of life. Flow cytometry was performed to determine the percentage of TCD4+ cells producing Th1 and Th2 cytokines. RESULTS: Supplementation with microalgae fatty acids decreased the percentage of TCD4+ cells producing IFN-γ and TNF-α and increased the ones producing IL-17A and IL-12 in both strains; on the other hand, supplementation decreased percentage of TCD4+ cells producing IL-4 and increased the ones producing TGF-ß. CONCLUSIONS: Microalgae n-3 fatty acids could be a useful tool in the treatment of diabetes as well as in the prevention of the appearance of health complications caused by inflammatory states.

VALORACIÓN DE ENERGÍA DIGESTIBLE DE LA GLICERINA CRUDA PROVENIENTE DE ACEITE DE PALMA -Elaeis guineensis- PARA CERDOS EN CRECIMIENTO EN FUNCIÓN DEL NIVEL DE ALMIDÓN DE MAÍZ EN LA DIETA / VALUATION OF DIGESTIBLE ENERGY OF CRUDE GLYCERIN FROM PALM OIL -Elaeis guineensis- FOR GROWING PIGS BASED ON DIETARY CORN STARCH LEVEL

Para determinar la energía digestible aparente (EDA) de la glicerina cruda (GC) proveniente del aceite de palma (Elaeis guineensis) en cerdos se realizaron dos experimentos (E). En E1 se determinó la EDA para GC con la técnica de la bolsa móvil de nailon (TBMN) utilizando ocho cerdos castrados con cánula duodenal. En E2 se determinaron la EDA y la energía metabolizable aparente (EMA) para GC, por el método convencional con indicador (MCI), con 10 cerdos castrados ubicados en jaulas metabólicas. En E1 y E2 se evaluaron, en un arreglo factorial 2 x 5, dos niveles de almidón de maíz (NA) en la dieta, 10% (NA10) y 12% (NA12), y cinco niveles de sustitución con GC (0%, 2,5%, 5,0%, 7,5% y 10%) en E1 en un diseño completo al azar y en E2 en un diseño de cuadrado latino. Para TBMN y MCI los datos se analizaron mediante regresión lineal múltiple y el metabolismo del nitrógeno en MCI como un diseño de cuadrado latino utilizando los módulos REG y GLM del paquete SAS. Por TBMN no hubo efectos (P > 0,05) del NA sobre la EDA de GC. La EDA de GC se calculó en 3.251 kcal/kg MS. La EDA de GC en MCI dependió de NA (P < 0,001) estimándose en 4.427,3 y 3.769,3 Kcal/kg MS para NA10 y NA12, respectivamente. Hubo interacción (P < 0,001) entre NA y GC para la cantidad de nitrógeno digestible, se redujo cuando GC aumentó en NA12 y aumentó en NA10. Otros parámetros del metabolismo nitrogenado no se vieron afectados (P > 0,05). Los resultados de EDA corregidos por NA con MCI mostraron correlación alta (R2 = 0,82) con TBMN. Con MCI se pudo establecer que el aumento en NA en la dieta reduce la EDA de GC.

To determine the apparent digestible energy (EDA) of crude glycerin (GC) from palm oil (Elaeis guineensis) in pigs two experiments (E) were performed. In E1, EDA was determined to GC, with the technique of mobile nylon bag (TBMN) using eight barrows with duodenal cannula. In E2, EDA, EMA to GC was determined by the conventional method with indicator (MCI) with 10 barrows placed in metabolic cages. In E1 and E2 were evaluated in 2 x 5 factorial arrangement, two levels of corn starch (NA) in the diet, 10 (NA10) and 12% (NA12), and five levels of replacement by GC (0%, 2.5%, 5%, 7.5% and 10%), in E1 as a completely randomized design and in E2 as a Latin square design. For MCI and TBMN, data were analyzed using multiple linear regression and nitrogen metabolism in MCI as a Latin square design using the GLM and REG modules of SAS. By TBMN there was no effect (P > 0.05) in the NA on the EDA of GC. EDA of GC was calculated at 3251 kcal/kg DM. EDA of GC in the MCI depended NA (P < 0.001), estimated at 4427.3 and 3769.3 Kcal/kg MS for NA10 and NA12, respectively. Interaction (P < 0.001) between NA and GC for the amount of digestible nitrogen was observed, was reduced when GC increased by NA12 and increased by NA10. Other nitrogen metabolism parameters were not affected (P > 0.05). The results of EDA corrected by NA with MCI showed high correlation (R² = 0.82) with TBMN. By MCI was established that increased in NA reduced the EDA of GC.

Introduction and Establishment of Pissodes castaneus (Coleoptera: Curculionidae) in the Andean Patagonia of Argentina.

The pine weevils that occur in plantations of Pinus spp. in Andean Patagonia of Argentina belong to the species Pissodes castaneus (De Geer), a Eurasian endemic species, according to the identification based on molecular and morphological characters. Sequences of the mitochondrial Cytochrome oxidase subunit I and nuclear genes (28 S rDNA and ITS2) were obtained for individuals of 13 afforestations, covering the entire distribution area of the established populations in the Andean Patagonia of Argentina. Sequence comparison with representative species of the genus (European, North American, and Chinese species) shows that Patagonian specimens are conspecific to those of P. castaneus sequenced from Europe. Phylogenetic analyses indicate that all terminals from Patagonia form a monophyletic unit without evident subclades, eliminating the possibility of existence of more than one species of Pissodes Germar in this area, including cryptic ones. Moreover, the very low genetic divergence between the Patagonian populations suggests that it is plausible that P. castaneus was introduced into Patagonia from just one location. Mitochondrial DNA analysis shows that Patagonian terminals group together with a French haplotype and are clearly separated from other P. castaneus individuals represented in our sample, and reveal that established populations in Andean Patagonia originated via a limited introduction.

Pollen:ovule ratio and its relationship with other floral traits in Papilionoideae (Leguminosae): an evaluation with Argentine species.

The pollen:ovule ratio (P/O) has traditionally been used as a rough estimator of plant breeding systems. It has been shown that plant breeding systems are associated with particular floral traits. In this study, we determined the P/O in 21 Leguminosae species from Argentina and explored relationships between P/O and taxonomic position, flower size, floral rewards, pollen presentation and pollination mechanisms. According to the results, 15 out of the 21 species classified were obligate xenogamous, although some of them have been recorded as facultative xenogamous in previous studies. There was a significant effect of taxonomic position (genus), reward type and pollination mechanism on P/O. Species offering only nectar as a floral reward (which were species with a brush mechanism) had a significantly lower P/O than species offering pollen or pollen and nectar. Species with the brush pollination mechanism had the lowest P/O, while species with valvular and pump mechanism had the highest P/O. However, pollen presentation (primary and secondary) and flower size did not have a significant effect on P/O. Our results demonstrate that P/O variability is determined by taxonomic position and pollination mechanism in this plant group.

Cirugía antirreflujo por laparoscopia y manifestaciones extraesofágicas / Laparoscopic antireflux surgery and extraesophageal manifestations

Introduccion; El reflujo gastroesofágico (RGE) es una enfermedad comun en la poblacion general. Es bien conocida su relacion con manifestaciones extraesofagicas como tos cronica, ahogos nocturnos, asma y otras de orden otorrinolaringologico que con el tratamiento farmacologico mejoran, pero con tendencia a recurrir una vez. este es suspendido. Con la cirugia se puede lograr un control completo. Además, ha probado ser segura, efectiva y de efectos durables, superiores a los del tratamiento medico. Metodologia; Se realizó un estudio prospectivo entre julio de 1997 y febrero de 2001, en 44 pacientes con reflujo gastroesofágico y manifestaciones extraesofagicas, que fueron operados con la tecnica laparoscopica de Nissen-Rossetti con el fin de evaluar su respuesta. Esta serie hace parte de otra de 118 pacientes operados con reflujo gastroesofágico. Resultados: Estos pacientes con manifestaciones extraesofagicas constituyen un poco más de la 1/3 parte de la serie total (37,2 por ciento). Hubo 24 hombres y 20 mujeres, para una relacion 1,2: 1. Las manifestaciones extraesofagicas más comunes fueron los espasmos gloticos, la tos, el asma, la carraspera y la disfonia. La mitad de los pacientes tenia algun grado de esofagitis (5 tenian esofago de Barret) a la endoscopia digestiva. Además de este metodo de examen, se utilizó la monitoria de pH esofágico de 24 horas para establecer el diagnostico. El seguimiento promedio fue de 15 meses (rango 1-41 meses). Se registro un porcentaje de éxito de 95,3 por ciento, pues 86,3 por ciento fueron clasificados como Visick I y 9 por ciento como Visick II. Conclusion: La cirugia antirreflujo tipo Nissen-Rosseti para el control de las manifestaciones extraesofagicas causadas por RGE patologico, presento muy buenos resultados en la serie motivo de este estudio.

Algunas repercusiones del tabaco sobre el nino y la produccion de leche de madres fumadoras. / Some repercussions of tabaco on the child and milk production of smoking mothers

Se estudiaron 64 ninos menores del ano de edad hijos de madres fumadoras y no fumadoras, encontrandose que el habito maternal de fumar repercute negativamente en la produccion lactea, peso y talla, asi como tambien en la incidencia de infecciones respiratorias bajas, sugiriendose que por lo menos durante la gestacion y periodo de lactancias la mujer no fume