Polluted water from an urban reservoir (Madín dam, México) induces toxicity and oxidative stress in Cyprinus carpio embryos.

The Madín Dam is a reservoir located in the municipalities of Naucalpan and Atizapán, in the metropolitan area adjacent to Mexico City. The reservoir supplies drinking water to nearby communities and provides an area for various recreational activities, including kayaking, sailing and carp fishing. Over time, the number of specimens of common carp has notably diminished in the reservoir, which receives direct domestic drainage from two towns as well as numerous neighborhoods along the Tlalnepantla River. Diverse studies have demonstrated that the pollutants in the water of the reservoir produce oxidative stress, genotoxicity and cytotoxicity in juvenile Cyprinus carpio, possibly explaining the reduction in the population of this species; however, it is necessary to assess whether these effects may also be occurring directly in the embryos. Hence, surface water samples were taken at five sites and pharmaceutical drugs, personal care products (especially sunscreens), organophosphate and organochlorine pesticides, and other persistent organic pollutants (e.g., polychlorinated biphenyls and polycyclic aromatic hydrocarbons) were identified. Embryos of C. carpio were exposed to the water samples to evaluate embryolethality, modifications in embryonic development, lipoperoxidation, the quantity of hydroperoxide and oxidized proteins, and antioxidant enzyme activity (superoxide dismutase, catalase and glutathione peroxidase). It was found that the polluted water of the Madín Dam gave rise to embryolethality, embryotoxicity, congenital abnormalities, and oxidative stress on the common carp embryos.

DNA damage and cytotoxicity induced on common carp by pollutants in water from an urban reservoir. Madín reservoir, a case study.

Madín Reservoir provides a substantial amount of drinking water to two municipalities close to Mexico City metropolitan area. However, it receives untreated wastewater discharges from domestic sources in the towns of Nuevo Madín and others, as well as diverse pollutants which are hauled by the Río Tlalnepantla from its upper reaches, so that the xenobiotics in the reservoir are highly diverse in terms of type and quantity. Previous studies showed that MR is contaminated with xenobiotics such as Al, Hg and Fe, as well as NSAIDs, at concentrations exceeding the limits established for aquatic life protection. These pollutants have been shown to induce oxidative stress on Cyprinus carpio and may therefore also damage the genetic material of exposed organisms, eliciting cytotoxicity as well. The present study aimed to determine the genotoxicity and cytotoxicity induced on blood, liver and gill of C. carpio by the pollutants present in MR water. Specimens were exposed to water from five sampling sites and the following biomarkers were evaluated: DNA damage by comet assay, frequency of micronuclei, apoptosis by TUNEL assay and caspase-3 activity. Significant increases relative to the control group (P < 0.05) were found with all biomarkers in all tissues evaluated, with the level of damage differing between sampling sites. In conclusion, pollutants present in MR water are genotoxic and cytotoxic to C. carpio, and this sentinel species, coupled with the biomarkers evaluated herein, is a reliable tool for assessing the health risk to wildlife posed by exposure to pollutants in freshwater bodies.

Oxidative stress and genotoxicity induced by ketorolac on the common carp Cyprinus carpio.

The nonsteroidal anti-inflammatory drug ketorolac is extensively used in the treatment of acute postoperative pain. This pharmaceutical has been found at concentrations of 0.2-60 µg/L in diverse water bodies around the world; however, its effects on aquatic organisms remain unknown. The present study, evaluated the oxidative stress and genotoxicity induced by sublethal concentrations of ketorolac (1 and 60 µg/L) on liver, brain, and blood of the common carp Cyprinus carpio. This toxicant induced oxidative damage (increased lipid peroxidation, hydroperoxide content, and protein carbonyl content) as well as changes in antioxidant status (superoxide dismutase, catalase, and glutathione peroxidase activity) in liver and brain of carp. In blood, ketorolac increased the frequency of micronuclei and is therefore genotoxic for the test species. The effects observed were time and concentration dependent. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1035-1043, 2016.

Removal of methyl parathion in water, by Dugesia dorotocephala.

The aim of this study was to determine the efficiency of Dugesia dorotocephala on Methyl parathion removal. An initial concentration of 1.25 microg mL(-1) of MeP was used to evaluate the removal capacity of planarian. A first-order removal kinetics was obtained with a disappearance rate constant (k(r)) of 0.49 days(-1) and 69% efficiency on contaminant removal. This is significantly different (p < 0.5) from the degradation occurring in control systems, leading us to conclude that D. dorotocephala effectively removes MeP from contaminated water.

[The prescription of drugs in a dental clinic of a Mexican university hospital]. / Prescripción de medicamentos en una clínica odontológica de una universidad mexicana.

OBJECTIVE: To assess the drug prescription service in a dental clinic of a Mexican university hospital. METHOD: An observational, descriptive study was carried out which analysed 698 drugs prescribed for dental problems in 14 dental health care departments in our clinic between the period of January-June 2005. The following criteria were established: prescribed drug, indication, dosage, dosage interval, individualised treatment, treatment duration and potential drug interactions. Information taken from prescriptions and clinical records was compared with information from literature on the subject in order to determine the adequacy of prescription criteria. RESULTS: The most frequently prescribed drugs were paracetamol, naproxen, ampicillin and dicloxacillin (43.26, 15.38, 7.45 and 7.02%). The prescription criteria which showed the least adequacy were as follows: indication, dosage and dosage interval. The main potential drug interactions occurred between non-steroidal anti-inflammatory drugs and captopril/amoxicillin. CONCLUSIONS: Taking the above into consideration, it was determined that 37.25% of prescriptions were inadequate. This study has helped to establish strategies which will facilitate the appropriate use of drugs in the future.

Prescripción de medicamentos en una clínica odontológica de una universidad mexicana / No disponible

Objetivo: Evaluar la prescripción de medicamentos en una clínica odontológica de una universidad mexicana. Método: Mediante un estudio observacional y descriptivo, se analizaron 698 prescripciones odontológicas en 14 servicios clínicos que conforman la clínica en estudio, enfocados a la conservación y restauración de la salud bucal en enero-junio 2005. Se registraron criterios como: medicamento prescrito, indicación, dosis, intervalo de dosificación, individualización de la terapia, duración de tratamiento y presencia de interacciones farmacológicas potenciales. Para determinar la inadecuación en los criterios de prescripción se comparó la información obtenida en recetas y expedientes clínicos, con la de la literatura especializada. Resultados: Los medicamentos más prescritos fueron paracetamol, naproxeno, ampicilina y dicloxacilina en 43,26, 15,38, 7,45 y 7,02%. La indicación, dosis e intervalo de dosificación fueron los criterios con mayor inadecuación en la prescripción. Las principales interacciones potenciales fueron entre los antiinflamatorios no esteroideos con el captopril y la amoxicilina. Conclusiones: Con lo anterior, se determinó que el 37,25% de las prescripciones fueron inadecuadas. A través de este estudio se establecieron estrategias que permitirán en un futuro tener una política de uso racional de los medicamentos empleados

Objective: To assess the drug prescription service in a dental clinic of a Mexican university hospital. Method: An observational, descriptive study was carried out which analysed 698 drugs prescribed for dental problems in 14 dental health care departments in our clinic between the period of January-June 2005. The following criteria were established: prescribed drug, indication, dosage, dosage interval, individualised treatment, treatment duration and potential drug interactions. Information taken from prescriptions and clinical records was compared with information from literature on the subject in order to determine the adequacy of prescription criteria. Results: The most frequently prescribed drugs were paracetamol, naproxen, ampicillin and dicloxacillin (43.26, 15.38, 7.45 and 7.02%). The prescription criteria which showed the least adequacy were as follows: indication, dosage and dosage interval. The main potential drug interactions occurred between non-steroidal anti-inflammatory drugs and captopril/amoxicillin. Conclusions: Taking the above into consideration, it was determined that 37.25% of prescriptions were inadequate. This study has helped to establish strategies which will facilitate the appropriate use of drugs in the future