Color match analysis of single-shade and multi-shade composite resins using spectrophotometric and visual methods after bleaching.

The aim of this study is to evaluate the color match of the materials after the staining and bleaching procedures applied to the teeth restored with single-shade and multi-shade composite resins. Cavities prepared on the facial surface of human incisors were restored with two different composite resins. After 24 h, the color matching parameters between the initial filling-tooth of the samples were recorded. Then, the color measurements of the samples subjected to the coloring process were repeated. Each composite resin group was randomly divided into 2 subgroups and home and office bleaching methods were applied to the samples. After bleaching, color measurements were repeated. In terms of periods, a statistically significant difference was found in ΔE values 1 day and 28 days after bleaching (p<0.05). In addition, it was found that the color match between filling-tooth was generally weaker in Omnichroma material and after home bleaching.

The Evaluation of Different Polishing Techniques' Effects on the Post-operative External Staining of Enamel in Primary and Permanent Tooth.

Objective This study aimed to examine patient complaints on recoloration development after polishing applications in primary and permanent teeth that differed in enamel composition and to determine the ideal polishing method. Methods A total of 30 permanent upper incisors and 30 primary molars were randomly divided into three groups of 10 using three different polishing techniques. Each polishing method (rubber, brush, and air polishing) was applied to the test surface of its own group. Milk and coffee were used in the coloring processes. A spectrophotometer was used for color measurements. Color change (∆E) was calculated between control and test surfaces and between the three measurement points. Results In the primary teeth's test surfaces, the rubber and brush groups were significantly more colored than the air-polishing group, when compared between after polishing and after coloration (p ˂ 0.05). Furthermore, the color difference of the permanent teeth between the initial measurements and after coloration was significantly higher in the rubber group's test surface compared to the air-polished group (p ˂ 0.05). The average ∆E values in both primary and permanent teeth were as follows: rubber > brush > air polishing. Conclusions Compared to rubber or brush polishing, air polishing seems safer to avoid predisposition to postoperative enamel discoloration. Primary teeth are more colored than permanent teeth. The effect of polishing on postoperative coloring should always be considered, and air polishing should be preferred whenever possible.

Release of mercury from amalgam filling and its relationship with metallothionein and superoxide dismutase.

Purpose: This study aims at determining the amount of mercury released over time from amalgam after treatment in healthy subjects and to examine the relation of mercury with serum MT-1 and SOD-1 levels. Materials and methods: Amalgam filling was applied to the 15 subjects aged 19-22 years and blood samples were collected before treatment and 1 day, 7 days, 21 days and 35 days after treatment. Mercury analysis of serum samples was performed using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). In addition, MT-1 and SOD-1 levels in serum samples were measured using commercial enzyme-linked immunosorbent assay (ELISA). Friedman test and Spearman's correlation analysis was performed to analyse the data. p value was interpreted in significance level of 0.05. Results: As a result of the analysis for MT-1, it was found that the values decreased over time and this decrease was statistically significant after 21 days (p<0.05). In addition, it was found that SOD-1 decreased over time, but this decrease was not statistically significant . In terms of released mercury, there was no statistically significant difference among the values of mercury released over time . According to the results of correlation analysis, no statistically significant relationship was found among the variables. Conclusion: The results of the present study indicated that the amount of mercury released from the tested amalgam were found to be tolerable and no significant relationship was found between MT-1 and SOD-1.

Susceptibility to discoloration of dental restorative materials containing dimethacrylate resin after bleaching.

The aim of this study is to compare the susceptibility to discoloration of dental restorative materials containing dimethacrylate resin after bleaching. In this study, resin-modified glass ionomer, polyacid-modified composite resin, giomer, posterior composite resin, anterior composite resin, bulk fill composite resin, flowable bulk fill composite resin, ormocer, indirect composite resin and hybrid ceramics were used as restorative material containing dimethacrylate resin. 20 samples were prepared from each material in accordance with the manufacturer's instructions. After the baseline colors are measured, the samples were randomly divided into four subgroups as office bleaching, home bleaching and a separate control group for each type bleaching method. Then, the samples were bleached and colored. Color measurements of the samples were repeated after exposure to bleaching and coloring. Then, ΔE00 and whiteness index (WID) were calculated. Data were analyzed using Paired-Samples T Test, two-way ANOVA, Repeated Measures ANOVA and Tukey post-hoc tests. Statistical significance level was taken as p < 0.05. In terms of bleaching systems, Opalescence Boost was found to cause more whitening in materials but more discoloration (p < 0.05). In terms of materials, it was found that the most susceptible materials to coloration were Clearfil Majesty Posterior, Filtek Bulk Fill Posterior Restorative, Filtek Bulk Fill Flowable and Fuji II LC, while the least susceptible materials were CAD/CAM materials (p < 0.05). The bleaching process increases the susceptibility to coloration of materials containing dimethacrylate resin.

Opinions of Prospective Dentists and Prospective Teachers on Antibiotic Use.

The purpose of this study was to examine the opinions of prospective dentists and prospective teachers on antibiotic use. In this study, a survey method based on a quantitative research paradigm was utilized. A 19-item instrument was applied to the sample group including 414 university students attending to the Faculty of Dentistry and Faculty of Education. The instrument was composed of three main subscales in addition to demographic characteristics. The first part focused on attitudes, the second part focused on subjective norms, and the third part focused on the prospective dentists and prospective teachers' intentions regarding the phenomenon of antibiotics use. While the dependent variables of the study were the participants' levels of agreement in the subscales of the instrument, the independent variable was faculty studied. The data were analyzed through descriptive statistics, and the Mann-Whitney U test was used to compare the participants' views on antibiotic use. The findings in terms of the faculties showed that dentistry and education faculties in our country prepare well in expanding their reasonable consumption of antibiotics. Yet, antibiotics are used more incorrectly by some prospective teachers than by prospective dentists. According to the findings in terms of subscales, the participants have positive attitudes toward the reasonable use of antibiotics in terms of the attitude subscale. However, the results from the subjective norm indicated that participants are influenced by their environment in their antibiotic use. Finally, the results from the intention indicated that participants avoid using antibiotics irregularly or frequently.

The effects of different antioxidant agents on the microtensile bond strength of composite resin to bleached Enamel / Efecto de diferentes agentes antioxidantes en la resistencia a la microtracción de la resina compuesta al esmalte blanqueado

Abstract The objective of this in vitro study is to examine the effect of antioxidants on the bonding strength of composite resin to bleached enamel. One hundred twenty non decay teeth were used in this study. Specimens were cut from the enamel cement junction. Then the specimens were randomly assigned into two groups-office bleaching and home bleaching. Bleaching antioxidants were applied to the first 12 subgroups. The specimens in the remaining 12 groups were kept in artificial saliva for 14 days and antioxidants were applied. Composite resin restoration was applied to all specimens. A cutting device was employed to obtain six specimens (0.9mm) from each tooth, and the microtensile bond strength (μTBS) test was performed. Data were analyzed using one-way ANOVA, the paired t-test, and Duncan's post hoc test (p=0.05). In office bleaching specimens, immediate antioxidant application increased the μTBS values (p0.05). Mean μTBS values in the groups to which antioxidants were applied two weeks after home bleaching were higher than those in the groups in which antioxidants were applied immediately. The use of antioxidants after bleaching may increase the MTBS value. The clinician should take this into account especially in immediate restoration applications after bleaching.

Resumen El objetivo de este estudio in vitro fue examinar el efecto de los antioxidantes sobre la resistência adhesiva de la resina compuesta al esmalte blanqueado. En este estudio se utilizaron ciento veinte dientes sin caries. Se cortaron especímenes a nivel de la unión esmalte-cemento. A continuación, los especímenes se asignaron aleatoriamente a dos grupos: blanqueamiento en el consultorio y blanqueamiento en casa. Se aplicaron antioxidantes de blanqueamiento a los primeros 12 subgrupos. Los especímenes de los 12 grupos restantes se mantuvieron en saliva artificial durante 14 días y se aplicaron antioxidantes. Se aplicó una resina compuesta a todos los especímenes empleando un dispositivo de corte para obtener seis especímenes (0,9mm) de cada diente, y se realizó la prueba de resistencia a la adhesión por microtensión (μTBS). Los datos se analizaron mediante un ANOVA de una vía, la prueba t pareada y la prueba post hoc de Duncan (p=0,05). En las muestras de blanqueamiento de oficina, la aplicación inmediata de antioxidantes aumentó los valores de μTBS (p0,05). Los valores medios de μTBS en los grupos a los que se aplicaron antioxidantes dos semanas después del blanqueo en casa fueron mayores que los de los grupos en los que se aplicaron antioxidantes inmediatamente. El uso de antioxidantes después del blanqueamiento puede aumentar el valor de μTBS. El clínico debe tenerlo en cuenta, especialmente en las aplicaciones de restauración inmediatas tras el blanqueamiento.

Monomer release from dental restorative materials containing dimethacrylate resin after bleaching.

OBJECTIVE: The aim of this study is to determine the type and amount of monomer released over time after bleaching was applied to dental restorative materials containing dimethacrylate resin. MATERIALS AND METHODS: Thirteen different restorative materials containing dimethacrylate resin were used in this study. Twenty samples were prepared from each material and the samples were randomly divided into 4 subgroups, namely, office bleaching, home bleaching, and separate control group for each of the bleaching methods. After bleaching, the samples were stored in 75% ethanol solution for 1, 14, and 28 days. Analysis of the samples collected to determine the type and amount of residual monomer released from the materials was performed using a liquid chromatography-tandem mass/mass spectrometer (LC-MS/MS). Data were analyzed using Friedman and Kruskal-Wallis tests. Statistical significance level was taken as p < 0.05. RESULTS: In terms of bleaching groups, mostly no significant difference was found between the groups in each time period in restorative materials (p > 0.05). In terms of time periods, it was determined that the amount of release of all monomers was decreased over time. In terms of restorative materials, the highest monomer release was detected in composite resin groups and the least monomer release was detected in CAD/CAM blocks. CONCLUSION: Although monomers were released from all materials, bleaching did not affect the amount of released monomer. Amounts of released monomers were generally below toxic limits. CLINICAL RELEVANCE: Within the limits of this study, bleaching does not significantly affect monomer release in restorative materials containing dimethacrylate resin.

Effects of whitening toothpastes on the esthetic properties and surface roughness of a composite resin.

PURPOSE: The aim of this study was to investigate and compare the effects of some whitening toothpastes on the surface roughness and coloration susceptibility of a composite resin, as well as their whitening effectiveness. METHODS: This study was carried out in two different stages. In experiment A, samples were divided into 7 subgroups to compare the surface roughness and coloration susceptibility of the composite resin: distilled water, conventional toothpaste, and 5 different whitening toothpastes. In experiment B, samples were divided into 8 subgroups to compare the whitening effectiveness of the whitening toothpastes: conventional toothpaste, 5 other whitening toothpastes, and two bleaching groups. After toothbrushing simulation, the color and surface roughness of the samples were measured. Data were subjected to one-way analysis of variance, paired t-test and Tukey post-hoc test (α = 0.05). RESULTS: There were no statistically significant differences among the surface roughness values for the groups for any period before and after brushing (P > 0.05). In terms of the coloration susceptibility of the composite resin and the whitening effects of the toothpastes, no statistically significant differences were evident among the groups for any period before and after brushing (P > 0.05). CONCLUSION: The results of this study indicate that effective toothbrushing is more important than the type of toothpaste employed.

Effect of staining and bleaching on the microhardness, surface roughness and color of different composite resins.

BACKGROUND: There are studies that examined the effect of staining on the surface properties of composite resins, using different solutions and bleaching applications. However, the effect of both staining and bleaching on the same composite specimens is an issue that needs to be investigated. OBJECTIVES: The aim of this study was to investigate the surface microhardness, roughness and color changes (ΔE) of 2 different composite resins after staining and bleaching. MATERIAL AND METHODS: A microhybrid and a nanohybrid composite were used in the study. One hundred and fifty specimens were prepared from each composite. The specimens were divided into 5 groups and stained for 30 days with tea, coffee, cola, red wine, or distilled water (control). Subsequently, each group was divided into 3 subgroups. Each subgroup received a 14-day application of Opalescence™ Boost, Opalescence PF or VivaStyle® Paint On Plus bleaching materials. The color as well as surface microhardness and roughness of all specimens were determined at baseline, after staining and after bleaching. Data was analyzed using the repeated-measures analysis of variance (ANOVA) and the Bonferroni method. RESULTS: A statistically significant decrease was observed in the surface microhardness of the microhybrid composite specimens after bleaching (p < 0.05).The highest ΔE values were observed in the red wine groups for both composite resins. CONCLUSIONS: Staining and bleaching may affect surface properties and color, depending on the type, filler and matrix content of the composite resin.

Effect of coating on the color and surface hardness of the surface of dental ceramics.

BACKGROUND: The aim of this study was to investigate whether boron nitride (BN) and BN-chromium (BN-Cr) coatings applied with the sputtering method would change the characteristics of hardness and discoloration of the ceramic veneer surface. MATERIALS AND METHODS: In this experimental study, thirty disc-shaped cores (10 mm in diameter and 0.8 mm in thickness) were prepared. Three different ceramic systems, IPS Empress (E) (Ivoclar Vivadent, Schaan, Liechntenstein), IPS e.max Press (EP) (Ivoclar Vivadent, Schaan, Liechntenstein), and Turkom Cera (T) (Turcom-Ceramic SDN-BHD, Kuala Lumpur, Malaysia), were tested, each with n = 10. The specimens were divided into two subgroups (n = 5) according to the coating on the veneering ceramic: BN or BN-Cr. The Vickers hardness and color differences (ΔE) values were measured before and after coating. Surface analysis was performed with X-ray diffraction, X-ray photoelectron spectroscopy, and scanning electron microscopic. The Wilcoxon signed-rank test was performed to compare hardness values. The Kruskal-Wallis test was performed to compare ΔE values among all groups. The post hoc tests was used after Kruskal-Wallis, with level of statistical significance set at P ≤ 0.05. RESULTS: BN-Cr coating statistically significantly increased the hardness of all types of ceramics (P < 0.05). BN coating did not change the hardness statistically significantly (P > 0.05). The mean ΔE values indicated clinically noticeable (over the limit of 3.7) color change in all groups except for the EP-BN and T-BN groups. CONCLUSION: Although BN-Cr coating in this thickness had a negative effect on color, it increased the hardness of the ceramics and could be useful in the palatal region.

Evaluation of the color stability of temporary materials produced with CAD/CAM.

BACKGROUND: If a temporary restoration is in the esthetic area and needs to be worn for a long time, the color stability of temporary materials becomes an important factor. OBJECTIVES: The aim of this in vitro study was to evaluate the long-term effects of various staining solutions on the color stability of different temporary materials produced with the computer-aided design and computer-aided manufacturing (CAD/CAM) technology. MATERIAL AND METHODS: In the study, the following materials were used: VITA CAD-Temp® (group 1); Ceramill® Temp (group 2); and Telio® CAD (group 3). Forty disk-shaped specimens (10 mm in diameter, 2 mm in thickness) of each material (N = 120) were produced with a CAD/CAM system. Staining solutions - of tea (A), of coffee (B) and cola (C) - and distilled water (D, control) were used, and color was evaluated before and after storing the samples in the solutions. Measurements were taken with a spectrophotometer and the color parameters (L*, a*, b*, and ΔE) were calculated according to the Commission internationale de l'éclairage system (CIELab). The results were evaluated with the two-way analysis of variance (ANOVA) and Tukey's tests (α = 0.05). RESULTS: Clinically perceivable (ΔE00 > 0.8) and statistically significant (p < 0.001) color differences were detected in all specimens. The highest ΔE00 value was found in the Ceramill Temp specimens. In addition, the highest ΔE00 values were noted for the specimens stored in cola and the coffee solution for all groups. The lowest ΔE00 value was observed for the groups stored in the tea solution. CONCLUSIONS: Clinically perceivable color changes were observed in all the specimens kept in the solutions. Color changes were greater for cola and coffee as compared to tea.

Effects of Bisphenol A Released From Composite Fillings on Reproductive Hormone Levels in Men.

OBJECTIVES: Composite resins are the most preferred filling material because of their excellent aesthetic qualities. However, a filling material should also be biocompatible as well as aesthetic. The aim of this study was to determine the serum and saliva bisphenol-A (BPA) levels and to examine the effects of serum BPA on reproductive hormone levels after healthy men were treated with composite fillings. METHODS: Eighteen healthy males each received 2 composite restorations. Saliva and blood samples of subjects were collected before resin application and 1 day and 1, 3, and 5 weeks after the resin was applied. BPA amounts in samples were detected using high-performance liquid chromatography (HPLC). Serum gonadotropins, testosterone, sex hormone binding globulin, free androgen index, and oestrogen levels were measured with radioimmunological assay kits. Statistical analysis of data was made using Friedman, Wilcoxon signed ranks and Mann-Whitney U tests (α = 0.05). RESULTS: The amount of BPA released from composite resins over time was not significantly elevated in either saliva or serum (P > 0.5). In addition, serum BPA levels were significantly higher than saliva BPA levels for both composites (P < .05), but saliva and serum BPA levels were not statistically different when comparing the 2 composites (P > .05). CONCLUSIONS: BPA from composite resins used in this study did not significantly alter serum hormone levels.

Repair bond strength of resin composite to three aged CAD/CAM blocks using different repair systems.

PURPOSE: The purpose of this study is to evaluate the repair bond strength of a nanohybrid resin composite to three CAD/CAM blocks using different intraoral ceramic repair systems. MATERIALS AND METHODS: Three CAD/CAM blocks (Lava Ultimate, Cerasmart, and Vitablocks Mark II) were selected for the study. Thirty-two specimens were fabricated from each block. Specimens were randomly divided into eight groups for the following different intraoral repair systems: Group 1: control group (no treatment); Group 2: 34.5% phosphoric acid etching; Group 3: CoJet System; Group 4: Z-Prime Plus System; Group 5: GC Repair System; Group 6: Cimara System; Group 7: Porcelain Repair System; and Group 8: Clearfil Repair System. Then, nanohybrid resin composite (Tetric Evo Ceram) was packed onto treated blocks surfaces. The specimens were thermocycled before application of repair systems and after application of composite resin. After second thermal cycling, blocks were cut into bars (1 × 1 × 12 mm3) for microtensile bond strength tests. Data were analyzed using two-way ANOVA and Tukey's HSD test (α=.05). RESULTS: Cimara System, Porcelain Repair, and Clearfil Repair systems significantly increased the bond strength of nanohybrid resin composite to all CAD/CAM blocks when compared with the other tested repair systems (P<.05). In terms of CAD/CAM blocks, the lowest values were observed in Vitablocks Mark II groups (P<.05). CONCLUSION: All repair systems used in the study exhibited clinically acceptable bond strength and can be recommended for clinical use.

An evaluation of surface roughness after staining of different composite resins using atomic force microscopy and a profilometer.

AIM: The aim of this study was to compare the surface roughness of different composite resins using atomic force microscope (AFM) and a profilometer after storage in different solutions. MATERIALS AND METHODS: Eight different composite resins were used in this study. Twenty specimens of each composite resin material were prepared using a 2-mm thick and 8-mm diameter stainless steel mold. After the composites had been placed in the mold, they were polymerized with a LED curing unit. The surfaces of all specimens were polished using aluminum oxide discs, and the specimens were then divided into four groups. The specimens in the experimental groups were stored in cola, coffee, or red wine, while the control group was stored in distilled water. Specimen surface roughness was examined after 30 days using an AFM and a profilometer, and the data obtained were subjected to analysis. RESULTS: Evaluation of the surface roughness of composite resins using a profilometer revealed no statistically significant difference between the groups, but significant differences were found using the AFM. The mean surface roughness of nanohybrid composites was lower than that of microhybrid composites. CONCLUSIONS: The surface roughness of the composite resins varies with storage in different solutions, depending on the organic matrix structure and inorganic fillers of the resin.

Monomer release from bulk-fill composite resins in different curing protocols.

The purpose of this study was to determine the depth of cure and the type and amount of monomers released from bulk-fill composites in different curing protocols. Five different composite resins Filtek Bulk-Fill Posterior, Filtek Bulk-Fill Flowable, SureFil SDR, X-tra Fil, and X-tra base, were used. A light-emitting diode (LED) device was used in 3 different modes (standard, high power, and extra power mode), and a halogen light device was also used as a control. Surface hardness was measured and the depth of cure was calculated. Monomer analysis was performed using high performance liquid chromatography (HPLC). The data were analyzed using Tamhane's T2 post-hoc test (α = 0.05). The cure depth for all materials except for Filtek Bulk-Fill Posterior (extra power mode) and Filtek Bulk-Fill Flowable (high power and extra power modes) was over 80%. Under the conditions of this study, the amount of monomer released from composite resins changed according to the type of composite resin and the light mode used.

Monomer release from nanohybrid composites after bleaching.

The evaluation of the effect of bleaching on monomer release from two composite resins was performed by bleaching two nanohybrid composite resins Filtek Z550 and Tetric N-Ceram using two bleaching products Whiteness HP Maxx and Vivastyle. In total, 20 samples were made from each composite resin from which four groups were fabricated (two for each bleaching product). The samples were stored in a 75% ethanol solution, and the solutions were renewed after 1, 7, and 28 days. The monomer release was analyzed using high performance liquid chromatography. The data were analyzed using repeated measures analysis of variance and least significant difference multiple comparison test (α = 0.05). Monomers were found to be released from both composite resins. The amounts of monomer released were found to decrease over time (P < 0.05); however, the resins were not affected by bleaching, and the released monomers were well below toxic doses.

Anticariogenic potential of white cheese, xylitol chewing gum, and black tea.

OBJECTIVE: This study aimed to examine the effects of these foods on plaque pH and the potential development of tooth decay. MATERIALS AND METHODS: Plaque pH was measured using the sampling method before and after 1, 5, 10, 20, 30, 45, and 60 min following consumption of these foods individually and after rinsing with a 10% sucrose solution. Statistical analysis was performed using one-way ANOVA and Tukey honestly significant difference post hoc tests (α = 0.05). RESULTS: Although there were statistically significant differences in all test groups except the BT (P = 0.620) and sucrose + XCG (P = 0.550) groups in time, none of the participants chosen for this study were having a plaque pH value anywhere close to the critical value (pH = 5.5). CONCLUSION: WC, BT, and XCG are advisable as anticariogenic foods because pH values are not below critical value.

Comparison of radiopacity of different composite resins.

BACKGROUND: The radiopacity of composite resins has been considered as an important requirement, improving the radiographic diagnosis. AIM: The present study aimed to compare the radiopacity of eight different composite materials using an aluminum step wedge. MATERIALS AND METHODS: Eight different composite resins were used in this study. The samples were prepared using a stainless steel mold (2 × 8), and a 2-mm-thickness horizontal section was obtained from the freshly extracted molar tooth. Three different radiographs were taken by establishing standard conditions. Mean gray values were obtained by taking three measurements from each step of both the tooth and the aluminum step wedge, and the aluminum thickness equivalents were calculated. Statistical analysis was performed using one-way variance analysis and Tukey's test (P < 0.05). RESULTS: All aluminum thickness equivalents were found to be higher than those of the enamel and dentin, except Clearfil Majesty Esthetic (2.23 mm ± 0.52 mm) and Filtek Silorane (3.67 mm ± 0.15 mm) (P < 0.05). The Clearfil Majesty Posterior (8.50 mm ± 0.10 mm) and Arabesk Top (8.17 mm ± 0.06 mm) were found to be the most radiopaque composites. CONCLUSION: All composite resin materials tested in this study were confirmed to the International Standards Organization 4049 standards. However, since radiopacity is not the only criterion for clinical use, it is a better approach to take all other properties of the materials into consideration.

Lipid peroxidation and DNA oxidation caused by dental filling materials.

BACKGROUND/PURPOSE: The two direct dental restorative materials most commonly used today are silver-mercury amalgam and resin-based composites. The purpose of this study was to examine the effects of these two restorative materials and substances released by these into the oral environment on lipid peroxidation and DNA oxidation after entering the blood circulation. MATERIALS AND METHODS: Blood samples from 41 patients were collected before and 24 hours after the application of these restorative materials. The 8-hydroxydeoxyguanosine/deoxyguanosine ratio in these samples was measured to determine oxidative DNA damage, and malondialdehyde levels were measured to define lipid peroxidation. The paired samples t test and Pearson correlation analysis were used for the analysis of variables (α = 0.05). RESULTS: While no statistically significant difference was observed after amalgam filling application in terms of DNA oxidation, a significant difference was observed after composite application (P < 0.05). Furthermore, a significant increase was determined in malondialdehyde levels of two materials (P < 0.05). In both amalgam and composite applications, a significant difference was observed before and after application in terms of released substances (mercury and unpolymerized monomer, respectively, P < 0.001). CONCLUSION: Mercury increased lipid peroxidation and Bis-GMA and TEGDMA dental resins increased both lipid peroxidation and DNA oxidation markers.

Effects of composite restorations on nitric oxide and uric acid levels in saliva.

BACKGROUND AND AIMS: Dental materials that are used in dentistry should be harmless to oral tissues, and should, therefore, not contain any leachable toxic and diffusible substances capable of causing side effects. This study was intended to investigate the effects on salivary nitric oxide (NO) and uric acid (UA) levels after application of dental composite filling materials to healthy volunteers. MATERIALS AND METHODS: A total of 52 individuals (32 female and 20 male) participated in the study. Filtek Z250 composite filling material (3M ESPE, St Paul, MN, USA) was applied to healthy volunteers. Saliva samples were collected before restoration (baseline) and 1 h, 1-day, 7 days, and 30 days after restoration. NO concentrations were measured using the Griess reaction method, and UA was measured using an enzymatic method. Data were analyzed using repeated measures ANOVA and the Bonferroni post-hoc test (α =5%). RESULTS: NO values increased statistically significant after 7 days (P < 0.05). In addition, lower UA levels were determined compared to the baseline levels, but the difference was not statistically significant (P > 0.05). There was no correlation between NO and UA levels in saliva (P > 0.05). CONCLUSION: Composite resins activated the antioxidant system in saliva. However, further studies are now needed to confirm our findings and to permit a definitive conclusion.