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Virus Res ; 115(2): 207-13, 2006 Feb.
Article in English | MEDLINE | ID: mdl-16271277

ABSTRACT

Host RNA polymerase II (RNAP II) is responsible for viral transcription of the herpes simplex virus type 1 (HSV-1) genome and is relocalized to viral DNA replication compartments. Thus, we investigated whether TATA-binding protein (TBP) and TBP-associated factors (TAFs) are recruited to sites of viral transcription and replication and whether TBP/TAF expressions are influenced upon infection. The protein levels of TBP, hsTAF1/TAF(II)250, hsTAF4/TAF(II)135, and hsTAF5/TAF(II)100 were constant during the early phase of infection and started to decrease late during infection. Only for hsTAF7/TAF(II)55 we sometimes observed a decrease already at 4-8h postinfection (p.i.). Concomitantly with the relocalization of RNAP II, TBP and hsTAFs were redistributed to sites of viral DNA replication and transcription. In the absence of viral DNA replication TBP/hsTAFs were present in distinct nuclear dots, however, enlargement of the nuclear structures did not take place. Our results show that HSV-1 infection has no influence on the protein levels of TFIID components and leads to a redistribution of TBP and hsTAFs to prereplicative sites that enlarge to viral DNA replication compartments.


Subject(s)
Cell Nucleus/chemistry , DNA Replication , Herpesvirus 1, Human/physiology , TATA-Binding Protein Associated Factors/analysis , TATA-Box Binding Protein/analysis , Virus Replication , Cells, Cultured , DNA, Viral/metabolism , Fibroblasts , Humans , Immunohistochemistry , Microscopy, Confocal , RNA Polymerase II/analysis , Transcription, Genetic
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