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Placenta ; 44: 19-22, 2016 08.
Article in English | MEDLINE | ID: mdl-27452434

ABSTRACT

RT-qPCR requires a suitable set of internal control genes (ICGs) for an accurate normalization. The usefulness of 7 previously published ICGs in the human placenta was analyzed according to the effects of betamethasone treatment, sex and fetal age. Raw RT-qPCR data of the ICGs were evaluated using published algorithms. The algorithms revealed that a reliable normalization was achieved using the geometrical mean of PPIA, RPL19, HMBS and SDHA. The use of a different subset ICGs out of the 7 investigated, although not statistically affected by the conditions, biased the results, as demonstrated through changes in expression of glucocorticoid receptor (NR3C1) mRNA as a target gene.


Subject(s)
Genes, Essential/genetics , Glucocorticoids/pharmacology , Placenta/drug effects , Receptors, Glucocorticoid/genetics , Electron Transport Complex II/genetics , Female , Gene Expression Profiling , Glucocorticoids/therapeutic use , Humans , Hydroxymethylbilane Synthase/genetics , Peptidylprolyl Isomerase/genetics , Placenta/metabolism , Pregnancy , Ribosomal Proteins/genetics
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