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1.
Muscle Nerve ; 59(4): 509-516, 2019 04.
Article in English | MEDLINE | ID: mdl-30677146

ABSTRACT

INTRODUCTION: The aim of this study was to compare the effects of adenosine-5'-triphosphate (ATP) and adenosine on the contractility of rodent extensor digitorum longus (EDL) muscle at normal and low temperatures. METHODS: Contractions of rat and mouse isolated EDL were induced by either electrical stimulation (ES) or exogenous carbachol and recorded in the presence of ATP or adenosine (both at 100 µM). RESULTS: ATP at all temperatures caused a decrease of the contractions induced by carbachol in rat and mouse EDL and ES-induced contractions in rat EDL, while it potentiated the ES-induced contractions of mouse EDL. Adenosine reduced the contractility of rat and mouse EDL evoked by ES and did not affect the carbachol-induced contractions of rat and mouse EDL at any temperature. DISCUSSION: Under various temperature conditions, ATP inhibits pre- but potentiates postsynaptic processes in the mouse EDL; in the rat EDL ATP causes only inhibition of neuromuscular conduction. Muscle Nerve 59:509-516, 2019.


Subject(s)
Adenosine Triphosphate/pharmacology , Muscle Contraction/drug effects , Muscle Fibers, Fast-Twitch/drug effects , Animals , Carbachol/pharmacology , Cold Temperature , Electric Stimulation , Excitatory Postsynaptic Potentials/drug effects , Mice , Muscarinic Agonists/pharmacology , Muscle, Skeletal/drug effects , Neuromuscular Nondepolarizing Agents/pharmacology , Purinergic Agonists/pharmacology , Rats , Rats, Wistar , Tubocurarine/pharmacology
2.
Muscle Nerve ; 55(3): 417-423, 2017 03.
Article in English | MEDLINE | ID: mdl-27448234

ABSTRACT

INTRODUCTION: The aim of this study was to evaluate the effects of adenosine 5'-triphosphate (ATP) and adenosine on the contractility of mammalian skeletal muscle under hypothermic conditions. METHODS: Contractions of isolated rat soleus muscle were induced by either electrical stimulation (ES) or carbachol at physiological temperatures (37°C) and hypothermic conditions (30-14°C) and recorded in the presence of ATP, adenosine, suramin, and 8-(p-sulfophenyl)-theophylline (8-SPT). RESULTS: At 37°C, incubation of the muscles with ATP inhibited ES-induced contractions; the inhibitory effect of ATP disappeared at 14°C. Adenosine inhibited ES-induced contractions at all temperature levels; 8-SPT fully prevented the action of adenosine. ATP and adenosine did not significantly affect carbachol-induced contractions at 37°C, while at lower temperatures ATP potentiated them. Suramin fully prevented effects of ATP. CONCLUSIONS: ATP is involved in both pre- and postsynaptic regulation of rat soleus muscle contractility, and these processes are significantly more pronounced at low temperatures. Muscle Nerve 55: 417-423, 2017.


Subject(s)
Adenosine Triphosphate/pharmacology , Adenosine/pharmacology , Muscle Contraction/drug effects , Muscle, Skeletal/drug effects , Muscle, Skeletal/physiology , Temperature , Analysis of Variance , Animals , Carbachol/pharmacology , Cholinergic Agonists/pharmacology , Electric Stimulation , Hypothermia/chemically induced , Male , Nicotinic Antagonists/pharmacology , Purinergic P1 Receptor Antagonists/pharmacology , Purinergic P2 Receptor Antagonists/pharmacology , Rats , Rats, Wistar , Suramin/pharmacology , Theophylline/analogs & derivatives , Theophylline/pharmacology , Tubocurarine/pharmacology
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