Role of Th17 in the pathogenesis of cutaneous inflammatory diseases.

Th17 cells are a new T-cell subtype characterized by the capability of producing IL-17. They are reported to be involved in a wide range of cutaneous immune-mediated conditions and, particularly in this review, we sought to elucidate the Th17 role in the pathogenesis of some common inflammatory diseases including psoriasis, allergic contact dermatitis and atopic dermatitis.

New insights into the pathogenesis of cutaneous autoimmune disorders.

T helper 17 (Th17) cells are characterized by the secretion of IL-17, a proinflammatory cytokine. They represent a newly described T helper subpopulation that is distinct from Th1 and Th2 lineages. Because of their pleiotropic activity on fibroblasts, keratinocytes, endothelial cells, neutrophils and memory T cells, Th17 cells are thought to be crucial in mediating tissue inflammation and autoimmunity. Autoimmune diseases were classically considered as Th1-mediated disorders such as rheumatoid arthritis or mixed Th1/Th2 diseases such as inflammatory bowel diseases, systemic lupus erythematosus, bullous diseases, but new evidence suggests the deep involvement of Th17 cells in their pathogenesis that, potentially, may address a selective therapeutic approach targeting the IL23/Th17 pathway. This review summarizes the current knowledge of the pathogenic contribution of Th17 cells in select cutaneous autoimmune disorders, including lupus erythematosus, scleroderma, dermatomyositis, bullous pemphigoid and pemphigus vulgaris.

Extraintestinal Escherichia coli carrying virulence genes in coastal marine sediments.

Despite the recognized potential of long-term survival or even growth of fecal indicators bacteria (FIB) in marine sediments, this compartment is largely ignored by health protection authorities. We conducted a large-scale study over approximately 50 km of the Marche coasts (Adriatic Sea) at depths ranging from 2 to 5 m. Total and fecal coliforms (FC) were counted by culture-based methods. Escherichia coli was also quantified using fluorescence in situ hybridization targeting specific 16S rRNA sequences, which yielded significantly higher abundances than culture-based methods, suggesting the potential importance of viable but nonculturable E. coli cells. Fecal coliforms displayed high abundances at most sites and showed a prevalence of E. coli. FC isolates (n = 113) were identified by API 20E, additional biochemical tests, and internal transcribed spacer-PCR. E. coli strains, representing 96% of isolates, were then characterized for genomic relatedness and phylogenetic group (A, B1, B2, and D) of origin by randomly amplified polymorphic DNA and multiplex-PCR. The results indicated that E. coli displayed a wide genotypic diversity, also among isolates from the same station, and that 44 of the 109 E. coli isolates belonged to groups B2 and D. Further characterization of B2 and D isolates for the presence of 11 virulence factor genes (pap, sfa/foc, afa, eaeA, ibeA, traT, hlyA, stx(1), stx(2), aer, and fyuA) showed that 90% of B2 and 65% of D isolates were positive for at least one of these. Most of the variance of both E. coli abundance and assemblage composition (>62%) was explained by a combination of physical-chemical and trophic variables. These findings indicate that coastal sediments could represent a potential reservoir for commensal and pathogenic E. coli and that E. coli distribution in marine coastal sediments largely depends upon the physical and trophic status of the sediment. We conclude that future sampling designs aimed at monitoring the microbiological quality of marine coastal areas should not further neglect the analysis of the sediment and that monitoring of these environments can be improved by including molecular methods as a complement of culture-based techniques.

Feasibility studies for the treatment and reuse of contaminated marine sediments.

This paper presents preliminary results of laboratory tests aimed at evaluating the easibility of the remediation of marine sediments, which are polluted by mercury and petroleum hydrocarbons, dredged at the bay of Augusta (SR, Italy). The treatment is composed of two sequential steps: in the first, a cement-based granular material is produced (based on a high performance concrete approach); then, the volatile and the semi-volatile compounds in the granular material are removed by a thermal desorption step. Treated materials could be reused or put into caissons, according to their mechanical properties and environmental compatibility. The experiments were focused on evaluating the effect of the process parameter values on: (i) the evolution of cement hydration reactions, (ii) thermal desorption removal efficiencies, (iii) leaching behaviour of the treated material.

Metal content and toxicity of produced formation water (PFW): study of the possible effects of the discharge on marine environment.

A preliminary chemical and ecotoxicological assessment was performed on the produced formation water (PFW) and superficial sediment around a gas platform (Fratello Cluster), located in the Adriatic Sea (Italy), in order to evaluate the effects of PFW discharged from the installation. The ecotoxicological bioassays, with the marine bacterium Vibrio fischeri and the sea urchin Paracentrotus lividus, were associated with chemical data to estimate the possible effects on living organisms. PFW collected on the platform was toxic, but no significant effect was recorded on marine sediment. Only the sediment station nearest to the discharge point showed higher values of some contaminants (zinc and arsenic) in comparison to other sites and only some stations showed low toxicity.

A kinetic study of chelant-assisted remediation of contaminated dredged sediment.

A study on the remediation of a real heavy metal-contaminated sediment was conducted using the four chelating agents ethylenediaminetetraacetic acid (EDTA), nitrilotriacetic acid (NTA), citric acid and the S,S-isomer of the ethylenediaminedisuccinic acid ([S,S]-EDDS). Different chelant washing experiments were carried out at a chelant/total metal content of 1 and 10 mol/mol for treatment durations from 0.5 to 48 h to study the extraction kinetics of trace metals and major cations. The objective was to evaluate the removal efficiency of conventional chelating agents as compared to innovative, biodegradable species. Among the target contaminants, Cd, Cu, Pb and Zn were found to be adequately removed by both EDTA and EDDS, while NTA and citric acid yielded unsatisfactory results in some cases. It was also found that As was a critical contaminant which would require an additional specific treatment step, since the residual concentrations after the washing treatment were still far above the regulatory limit values.

DOTA tris(phenylmethyl) ester: a new useful synthon for the synthesis of DOTA monoamides containing acid-labile bonds.

The synthesis of DOTA tris(phenylmethyl) ester 2, a new monoreactive derivative of DOTA, is described. This versatile synthon can be easily coupled to compounds bearing an amino group and then deprotected to DOTA monoamide under mild and neutral conditions by catalytic hydrogenolysis. Accordingly, compound 2 has been used in the synthesis of a DOTA monoamide gadolinium complex containing two palmitic esters, which is a component of mixed micelles as MRI contrast agents.