ABSTRACT
Proviral DNA from non-producer Rous sarcoma in Syrian hamster contains practically all the nucleotide sequences presented in 125I-labeled RNA from Rous sarcoma virus, Carr-Zilber strain. Virus-specific sequences consist of moderately reiterated and unique DNA regions. The amount of Rous sarcoma virus-specific provirus equivalents in hamster Rous sarcoma DNA is equal to 5.2 +/- 0.01. Experiments on transfection show that proviral DNA studied possesses biological activity in respect to cell transformation and virus production. The infectivity of DNA from hamster tumor does not depend on the expression of group-specific (gs) antigen in the recipient cells.
Subject(s)
DNA, Neoplasm/metabolism , DNA, Viral/metabolism , Sarcoma, Avian/metabolism , Animals , Avian Sarcoma Viruses/genetics , Avian Sarcoma Viruses/growth & development , Avian Sarcoma Viruses/metabolism , Base Sequence , Cell Transformation, Neoplastic , Cells, Cultured , Cricetinae , DNA/genetics , Mesocricetus , Nucleic Acid Hybridization , RNA, Viral/metabolism , TransfectionABSTRACT
Sex hormones (testosterone, estradiol) and thyroxine would activate irregularly the production of the specific group antigen (GS-antigen) of avian ribodesoxyviruses in the culture of "normal" chick embryonal cells (CEC, phenotype C/O, GS-). The mentioned hormones as well as hydrocortisone and insulin would not prevent the Rous sarcoma virus (RSV) infecting of CEC, render no essential action on the accumulation of RSV in the infected CEC culture, and failed to activate the formation of mature RSV in virogenic cultures of rat (XC, R--B77) and mice cells (DBA--B77).
Subject(s)
Hormones/pharmacology , Retroviridae/drug effects , Animals , Antigens, Viral , Avian Sarcoma Viruses/drug effects , Cells, Cultured , Chick Embryo , Epitopes , Estradiol/pharmacology , Genes, Viral/drug effects , Hydrocortisone/pharmacology , Mice , Rats , Testosterone/pharmacology , Thyroxine/pharmacology , Virus Cultivation , Virus Replication/drug effectsABSTRACT
The nuclear genome of Syrian hamster virrion non-producer tumor contains the Rous sarcoma virus-specific nucleotide sequences. The information complexity of the proviral DNA from its tumor is similar to that for the DNA of Rous chicken sarcoma. The proviral DNA both in the hamster and chicken tumor consists of moderately reiterated and unique sequences. The avian oncornavirus-specific sequences are absent in the DNA of normal hamster tissue.
Subject(s)
DNA, Neoplasm/analysis , DNA, Viral/analysis , Nucleic Acid Hybridization , Sarcoma, Avian/analysis , Animals , Avian Sarcoma Viruses , Base Sequence , Chickens , Chromosomes/analysis , Cricetinae , Genes , MesocricetusABSTRACT
The intracellular concentration of cyclic AMP in normal cells and in cells infected with the Rous sarcoma virus (chicken fibroblasts) was determined by the method of saturation analysis (method of concurrent binding with protein). Thyroxine (2 mcg/ml) increased the level of cyclic AMP both in normal and infected cells, insulin (2 mcg/ml) decreased the concentration of cyclic AMP in infected cells, but cholesterol (5 mcg/ml) exerted no effect on this parameter in infected cells. The data are discussed from the point of view of a possible influence of hormones on the cholesterol accumulation in cells and on the adenylatecyclase activity in cell membranes.
