ABSTRACT
Angiogenesis is the formation of new blood vessels from pre-existing vessels and normally occurs during the process of inflammatory reactions, wound healing, tissue repair, and restoration of blood flow after injury or insult. Stimulation of angiogenesis is a promising and an important step in the treatment of peripheral artery disease. Reactive oxygen species have been shown to be involved in stimulation of this process. For this reason, we have developed and validated a non-equilibrium atmospheric temperature and pressure short-pulsed dielectric barrier discharge plasma system, which can non-destructively generate reactive oxygen species and other active species at the surface of the tissue being treated. We show that this plasma treatment stimulates the production of vascular endothelial growth factor, matrix metalloproteinase-9, and CXCL 1 that in turn induces angiogenesis in mouse aortic rings in vitro. This effect may be mediated by the direct effect of plasma generated reactive oxygen species on tissue.
ABSTRACT
The research was aimed at studying the possibility of obtaining drug ointments with a potential anti-mycotic activity by using thick extracts obtained from the leaves of fustic, hazel, nut and bark of the oak. There were prepared the ointment compositions on different bases. As a methodology for studying the properties of the obtained ointments, there have been used the studies of colloidal stability and resorption of tanning substances in agar. The obtained results allow for making conclusion that the selected ointments are colloidally stable, and the values of movement of zones of biologically active substances in the agar body are large enough. These data allow for forecasting both the possibility of creating the similar ointment systems and their rather high properties. Based on the obtained data, there have been selected the ointment composition variants for further studies.
