Rapid screening and characterization of bacteria associated with hospital cockroaches (Blattella germanica L.) using MALDI-TOF mass spectrometry.

AIMS: The study aimed to explore the diversity of culturable microbiota colonizing the alimentary tract and outer surfaces of German cockroaches (Blattella germanica) captured in a health care facility. METHODS AND RESULTS: Microbial identification was conducted using Matrix Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Biotyper and 16S rRNA sequencing. A total of 181 bacteria strains were isolated from 25 cockroach specimens and the MALDI-TOF MS-based assay yielded direct identification of 96·5% (175 out of 181) of the strains at the species level. The proteomic fingerprinting mainly revealed strains belonged to Gram-negative Enterobacteria (103) with six different genera that were characterized including Citrobacter, Klebsiella, Kluyevera, Leclercia, Morganella and Serratia. In addition, Pseudomonas sp. strains ranked in second with 29·8% (54 strains) followed by Staphylococcus sp. (6·62%) and Enterococcus sp. (1·65%). A large number of these bacteria (n = 90, 49·72%) was found in cockroaches captured in the maternity ward, whereas 45 strains (24·8%) were recovered in the paediatric ward. Altogether, 24 bacterial species were identified from both the external surface and digestive tract of the insect, of which Serratia marcescens presented the major group (n = 80, 44·19%) followed by Pseudomonas aeruginosa (n = 53, 29·28%) and Klebsiella oxytoca (n = 9, 4·94%). CONCLUSION: The findings showed a high prevalence of bacterial pathogens harboured in the body and alimentary tract of B. germanica captured in a health care facility. SIGNIFICANCE AND IMPACT OF THE STUDY: This investigation shows the possible role of German cockroaches as a source for bacterial pathogens, increasing the likelihood of direct transmission of healthcare associated infections, and thereby representing a public health risk. In addition, the present study revealed a high discriminatory power of the mass spectra investigation and a competent bacterial typing tool that extends phenotypic and genotypic approaches, which allows new possibilities for fast and accurate diagnosis in medical entomology.

A non-polyenic antifungal produced by a Streptomyces yatensis strain isolated from Mellah Lake in El Kala, North-East of Algeria.

OBJECTIVE: This study aimed at describing one actinomycete strain E65 that was isolated from the water of Mellah Lake in El Kala, North-East of Algeria that produces a non-polyenic antifungal. MATERIALS AND METHODS: Actinomycetes were isolated from Mellah Lake water and screened for antimicrobial activity. Antimicrobial assays were performed on ISP2 agar. The taxonomic position of the strain E65 was determined regarding phenotypic and 16S DNA sequences features. Time course of antifungal metabolites production was evaluated against Candida albicans on ISP2, ISP1 and GYEA broth. The active antifungal compound was extracted using dichloromethane and revealed by a thin layer of chromatography, chemical reagents, UV-visible and infrared spectroscopy. RESULTS: A total of 104 actinomycetes were isolated and screened for antimicrobial activity; 21 strains were active against Staphylococcus aureus, Escherichia coli and Candida albicans. The strain E65 showed a high in vitro activity against S. aureus and C. albicans and a good antifungal activity against a clinical C. albicans strain resistant to 5-fluorocytosine. Its 16S rRNA sequence shared 99% similarity with the Streptomyces yatensis type strain within the Streptomyces violaceusniger subclade of the Streptomyces hygroscopicus clade. It produced a non-polyenic antifungal, the IR spectrum of the antifungal extract corresponded to none of the antimicrobials compounds known to be produced by actinomycete of the S. hygroscopicus clade. CONCLUSION: The wetlands of El Kala, Algeria are a potential source of bioactive actinomycete that deserves to be explored and exploited. The Streptomyces yatensis E65 strain isolated from Mellah Lake brackish water produces a remarkable antifungal compound which original non-polyenic structure warrants further characterization.

Emergence of the European ST80 clone of community-associated methicillin-resistant Staphylococcus aureus as a cause of healthcare-associated infections in Eastern Algeria.

AIMS: The authors had for aim to assess whether an in-hospital spread of the European community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) clone was on-going in Annaba, Eastern Algeria. MATERIAL AND METHODS: We carried out a molecular epidemiological study of S. aureus strains causing infections in 4 hospitals located in Annaba, between February and October 2010. RESULTS: Our study revealed a very low healthcare-associated MRSA (HCA-MRSA) infection incidence rate of 0.34 per 1000 patient-days. However, the rates of HCA-MRSA strains (85/119) and CA-MRSA (7/29) among S. aureus strains are much higher than those found in France. The European CA-MRSA clone (clonal complex 80, Staphylococcal Cassette Chromosome mec IVc, spa type t044, lukS/F-PV-positive) accounted for 14.1% of all healthcare-associated (HCA) MRSA infections. CONCLUSION: This study confirmed the emerging role of CA-MRSA as HCA pathogens in North-African Africa.