Exploring sustainable management by using green nano-silver to combat three post-harvest pathogenic fungi in crops.

Global crop protection and food security have become critical issues to achieve the 'Zero Hunger' goal in recent years, as significant crop damage is primarily caused by biotic factors. Applying nanoparticles in agriculture could enhance crop yield. Nano-silver, or AgNPs, have colossal importance in many fields like biomedical, agriculture, and the environment due to their antimicrobial potential. In this context, nano-silver was fabricated by Citrus medica L. (Cm) fruit juice, detected visually and by UV-Vis spectrophotometric analysis. Further, AgNPs were characterized by advanced techniques. UV-Vis spectroscopic analysis revealed absorbance spectra at around 487 nm. The zeta potential measurement value was noted as -23.7 mV. Spectral analysis by FT-IR proved the capping of the acidic groups. In contrast, the XRD analysis showed the Miller indices like the face-centered cubic (fcc) crystalline structure. NTA revealed a mean size of 35 nm for nano-silver with a 2.4 × 108 particles mL-1 concentration. TEM analysis demonstrated spherical Cm-AgNPs with 20-30 nm sizes. The focus of this research was to evaluate the antifungal activity of biogenic AgNPs against post-harvest pathogenic fungi, including Aspergillus niger, A. flavus, and Alternaria alternata. The Cm-AgNPs showed significant antifungal activity in the order of A. niger > A. flavus > A. alternata. The biogenic Cm-AgNPs can be used for the inhibition of toxigenic fungi.

Role of diosgenin extracted from Helicteres isora L in suppression of HIV-1 replication: An in vitro preclinical study.

Background: Diosgenin, an essential sapogenin steroid with significant biological implications, is composed of a hydrophilic sugar moiety intricately linked to a hydrophobic steroid aglycone. While the antiviral properties of diosgenin against numerous RNA viruses have been extensively documented, its potential in combating Human Immunodeficiency Virus infections remains unexplored. Experimental procedure: This current investigation presents a comprehensive and systematic analysis of extracts derived from the leaves of Helicteres isora, which are notably enriched with diosgenin. Rigorous methodologies, including established chromatographic techniques and Fourier-transform infrared spectroscopy were employed for the characterization of the active diosgenin compound followed by molecular interaction analyses with the key HIV enzymes and mechanistic validation of HIV inhibition. Key results: The inhibitory effects of extracted diosgenin on the replication of HIV-1 were demonstrated using a permissive cellular system, encompassing two distinct subtypes of HIV-1 strains. Computational analyses involving molecular interactions highlighted the substantial occupancy of critical active site pocket residues within the key HIV-1 proteins by diosgenin. Additionally, the mechanistic underpinnings of diosgenin activity in conjunction with standard controls were elucidated through specialized colorimetric assays, evaluating its impact on HIV-1 Reverse Transcriptase and Integrase enzymes. Conclusions: To our current state of knowledge, this study represents the inaugural demonstration of the anti-HIV efficacy inherent to diosgenin found in the leaves of Helicteres isora, and can be taken further for drug design and development for the management of HIV infection.

Cell free extract-mediated biogenic synthesis of ZnONPs and their application with kanamycin as a bactericidal combination.

Antimicrobial resistance (AMR) is a main public health issue and a challenge for the scientific community all over the globe. Hence, there is a burning need to build new bactericides that resist the AMR. The ZnONPs were produced by cell free extract of mint (Mentha piperita L.) leaves. Antibiotics that are ineffective against resistant bacteria like Escherichia coli and Staphylococcus aureus were treated. The antibiotics were first screened, and then antibacterial activity was checked by disk diffusion, and MIC of Mp-ZnONPs individually and using Kanamycin (KAN) were determined against these pathogens by broth microdilution method. The synergism between Mp-ZnONPs and KAN was confirmed by checkerboard assay. The MIC showed robust antibacterial activity against the tested pathogens. The combination of KAN and Mp-ZnONPs reduces the MIC of KAN as it efficiently inhibits E. coli's growth, and KAN significantly enhances the antibacterial activity of Mp-ZnONPs. Taken together, Mp-ZnONPs have strong antimicrobial activity, and KAN significantly improves it against the tested pathogens, which would offer an effective, novel, and benign therapeutic methodology to regulate the incidence. The combination of Mp-ZnONPs and KAN would lead to the development of novel bactericides, that could be used in the formulation of pharmaceutical products.

Phoma spp. an untapped treasure of cytotoxic compounds: current status and perspectives.

The genus Phoma has been explored for a wide range of secondary metabolites signifying a huge range of bioactivities. Phoma sensu lato is a major group that secretes several secondary metabolites. The genus Phoma mainly includes Phoma macrostoma, P. multirostrata, P. exigua, P. herbarum, P. betae, P. bellidis, P. medicaginis, P. tropica, and many more species from the genus that are continuously being identified for their potential secondary metabolites. The metabolite spectrum includes bioactive compounds like phomenon, phomin, phomodione, cytochalasins, cercosporamide, phomazines, and phomapyrone reported from various Phoma spp. These secondary metabolites show a broad range of activities including antimicrobial, antiviral, antinematode, and anticancer. The present review is aimed to emphasize the importance of Phoma sensu lato fungi, as a natural source of biologically active secondary metabolites, and their cytotoxic activities. So far, cytotoxic activities of Phoma spp. have not been reviewed; hence, this review will be novel and useful for the readers to develop Phoma-derived anticancer agents. KEY POINTS: • Different Phoma spp. contain a wide variety of bioactive metabolites. • These Phoma spp. also secrete cytotoxic and antitumor compounds. • The secondary metabolites can be used for the development of anticancer agents.

Biogenic Synthesis of Fluorescent Carbon Dots (CDs) and Their Application in Bioimaging of Agricultural Crops.

Fluorescent nanoparticles have a transformative potential for advanced sensors and devices for point-of-need diagnostics and bioimaging, bypassing the technical burden of meeting the assay performance requirements. Carbon dots (CDs) are rapidly emerging carbon-based nanomaterials. Regardless of their fate, they will find increasing applications. In this study, a simple approach for synthesizing CDs from fruit peels was developed. The CDs were fabricated from Annona squamosa (L.) peels using a carbonization technique through microwave-assisted hydrothermal digestion at temperatures around 200 °C. Synthesized CDs were detected using a UV transilluminator for the preliminary confirmation of the presence of fluorescence. UV-Vis spectrophotometry (absorbance at 505 nm) analysis, zeta potential measurement (-20.8 mV), nanoparticles tracking analysis (NTA) (average size: 15.4 nm and mode size: 9.26 nm), photoluminescence, and Fourier transform infrared (FT-IR) analysis were used to identify the capping functional groups on the CDs. The total quantum yield exhibited was 8.93%, and the field emission scanning electron microscopy (FESEM) showed the size range up to 40 nm. The germinating mung bean (Vigna radiata (L.)) seeds were incubated with biogenically synthesized CDs to check the absorption of CDs by them. The fluorescence was observed under a UV-transilluminator in the growing parts of seeds, indicating the absorption of CDs during the germination, development, and growth. These fluorescent CDs could be used as a bioimaging agent. This novel method of synthesizing CDs was found to be eco-friendly, rapid, and cost-effective.

In Situ Growth of Biocompatible Biogenic Silver Nanoparticles in Poly-Vinyl Alcohol Thin Film Matrix.

Green synthesis is an eco-friendly approach of nanoparticles fabrication gaining momentum among researches. Nanoparticles are used immensely, due to its small size, physical properties, orientation, which can apparently change the performance of any other material when in proximity. Antibacterial, antioxidant, antifungal, antiviral, anti-inflammatory activities of several nanoparticles capable of wound healing make it a appropriate module for wound dressing materials. Silver nanoparticles (SNPs) are recognized as well established antimicrobial and antiseptic agents, thus considering it as a promising candidate for wound healing process and other applications. Here we report an effective and biological approach of a novel thin film preparation based on polyvinyl alcohol (PVA) with SNPs generated within matrix using leaf extract of Aloe vera as a bioreducing agent. Successful incorporation of SNPs into the polymer matrix, which was confirmed by NTA analysis, TEM, SEM. The characterization results revealed that SNPs were found in range of 10-40 nm. Evaluation of antimicrobial activity on Escherichia coli (ATCC 39403), Staphylococcus aureus (ATCC 25923), Klebsiella pneumoniae (clinical isolates), and Candida albicans (diploid fungus) using agar cup and disc diffusion method confirmed effective performance of the PVA-SNPs film. Average ZOI was observed as 3+2mm, 3+2mm, 4+1mm and 4+1mm respectively. Thin film produced is highly biocompatible to HaCat and L929 cell lines for a defined amount and hence can be used as wound dressing materials. The method used in this study is greener, simple, rapid, and cost effective for producing a biocompatible film with profoundly variable applications in food packaging and health care industries.

Chitosan nanoparticles (ChNPs): A versatile growth promoter in modern agricultural production.

Agriculture is a backbone of global economy and most of the population relies on this sector for their livelihood. Chitosan as a biodegradable material thus can be explored for in various fields in its nano form to replace non-biodegradable and toxic compounds. The chitosan has appealing properties like biocompatibility, non-toxicity, biodegradability, and low allergenic, making it useful in several applications including in agriculture sector. Because of their unique properties, chitosan nanoparticles (ChNPs) are extensively applied as a bioagent in various biological and biomedical processes, including wastewater treatment, plant growth promoter, fungicidal agent, wound healing, and scaffold for tissue engineering. Furthermore, the biocompatibility of chitosan nanoparticles (ChNPs) is reported to have other biological properties such as anti-cancerous, antifungal, antioxidant activities, even induces an immune response in the plant, and helps manage biotic and abiotic stresses. Chitosan can also find its application in wastewater treatment, hydrating agents in cosmetics, the food industry, paper, and the textile industry as adhesive, drug-delivering agent in medical as well as for bioimaging. Since chitosan has low toxicity, the nano-formulation of chitosan can be used for the controlled release of fertilizers, pesticides, and plant growth promoters in agriculture fields. The ChNPs applications in precision farming being a novel approach in recent developments. Here we have comprehensively reviewed the major points in this review are; the synthesis of ChNPs by biological resources, their modification and formulation for increasing its applicability, their modified types, and the different agricultural applications of ChNPs.

Superior in vivo Wound-Healing Activity of Mycosynthesized Silver Nanogel on Different Wound Models in Rat.

Wound healing is a complex phenomenon particularly owing to the rise in antimicrobial resistance. This has attracted the attention of the scientific community to search for new alternative solutions. Among these, silver being antimicrobial has been used since ancient times. Considering this fact, the main goal of our study was to evaluate the wound-healing ability of mycofabricated silver nanoparticles (AgNPs). We have focused on the formulation of silver nanogel for the management of wounds in albino Wistar rats. Mycosynthesized AgNPs from Fusarium oxysporum were used for the development of novel wound-healing antimicrobial silver nanogel with different concentrations of AgNPs, i.e., 0.1, 0.5, and 1 mg g-1. The formulated silver nanogel demonstrated excellent wound-healing activity in the incision, excision, and burn wound-healing model. In the incision wound-healing model, silver nanogel at a concentration of 0.5 mg g-1 exhibited superior wound-healing effect, whereas in the case of excision and burn wound-healing model, silver nanogel at the concentrations of 0.1 and 1 mg g-1 showed enhanced wound-healing effect, respectively. Moreover, silver nanogel competently arrests the bacterial growth on the wound surface and offers an improved local environment for scald wound healing. Histological studies of healed tissues and organs of the rat stated that AgNPs at less concentration (1 mg g-1) do not show any toxic or adverse effect on the body and promote wound healing of animal tissue. Based on these studies, we concluded that the silver nanogel prepared from mycosynthesized AgNPs can be used as a promising antimicrobial wound dressing.

Promising antimicrobials from Phoma spp.: progress and prospects.

The increasing multidrug-resistance in pathogenic microbes and the emergence of new microbial pathogens like coronaviruses have necessitated the discovery of new antimicrobials to treat these pathogens. The use of antibiotics began after the discovery of penicillin by Alexander Fleming from Penicillium chrysogenum. This has attracted the scientific community to delve deep into the antimicrobial capabilities of various fungi in general and Phoma spp. in particular. Phoma spp. such as Phoma arachidicola, P. sorghina, P. exigua var. exigua, P. herbarum, P. multirostrata, P. betae, P. fimeti, P. tropica, among others are known to produce different bioactive metabolites including polyketides, macrosporin, terpenes and terpenoids, thiodiketopiperazines, cytochalasin derivatives, phenolic compounds, and alkaloids. These bioactive metabolites have already demonstrated their antimicrobial potential (antibacterial, antifungal, and antiviral) against various pathogens. In the present review, we have discussed the antimicrobial potential of secondary metabolites produced by different Phoma species. We have also deliberated the biogenic synthesis of eco-friendly antimicrobial silver nanoparticles from Phoma and their role as potential antimicrobial agents.

Management of Phytophthora parasitica causing gummosis in citrus using biogenic copper oxide nanoparticles.

AIM: The main aim of the present study was to develop nanotechnology-based solutions for the management of a fungus, Phytophthora parasitica causing gummosis in citrus. METHODS AND RESULTS: Biogenic copper nanoparticles (CuONPs) were synthesized using two different biocontrol agents, Pseudomonas fluorescens and Trichoderma viride and characterized using different analytical techniques. Furthermore, in vitro (at the concentrations of 10, 15, 30, 50, 70, 100 and 150 mg/L) and in vivo (at the concentration of 100 mg/L) activities of these nanoparticles were evaluated for their antifungal efficacy against P. parasitica. The results obtained confirmed the synthesis of irregular-shaped CuONPs having a size in the range 40-100 nm in case of P. fluorescens, whereas, spherical CuONPs in the size range 20-80 were recorded in case of T. viride. As far as the in vitro antifungal efficacies of both these CuONPs is concerned, the maximum percent growth inhibition was observed in case of CuONPs synthesized from T. viride compared to CuONPs from P. fluorescens. However, in case of in vivo antifungal efficacies, CuONPs synthesized from T. viride showed the activity significantly higher than the conventionally used Bordeaux mixture. CONCLUSIONS: It can be concluded that biosynthesized CuONPs can be effectively used as a potential fungicide against P. parasitica. SIGNIFICANCE AND IMPACT OF THE STUDY: The application of nanoparticles having antifungal activities can be used as alternative fungicides to the conventional chemical fungicides. It has the potential to revolutionize the existing management strategies available for plant pathogenic fungi.

Assessment of in vitro antimicrobial efficacy of biologically synthesized metal nanoparticles against pathogenic bacteria.

The microbial infections due to biofilm forming bacterial pathogens are very common in human subjects. The intensive application of antibiotics in integrated disease management strategy has led to increased multidrug resistance incommon pathogens. Thus, indicating need of developing an alternative method for the control of these multidrug resistant pathogens. Present study involves the Moringa oleifera aqueous extract mediated biological synthesis of silver (Ag nanoparticles (NPs)- Avg. size 82.5 nm; zeta potential = -27.9 mV), copper oxide (CuONPs- Avg. size 61 nm; zeta potential = -19.3 mV), iron oxide (FeONPs- Avg. size 83.3 nm; zeta potential = -9.37 mV) and alumina (AlONPs- Avg. size 87.3 nm; zeta potential = -10.9 mV) nanoparticles. Biological nanoparticles were detected by visual observation, spectrophotometric detection followed by zeta potential analysis, nanoparticle tracking analysis, Fourier transform infrared spectrometry and X-ray diffraction analysis. Nanoparticles were further evaluated for their in vitro antimicrobial potential, membrane damage effectiveness, biofilm inhibition activity by MTT assay. Nanoparticles were assessed against human pathogens viz. two Gram-positive (Bacillus subtilis MTCC 441 and Staphylococcus haemolyticus MTCC 3383) and two Gram-negative bacteria (Enterobacter aerogenes MTCC 111 and Salmonella enterica ser. Typhi MTCC 8767). The nanoparticles exhibited akin activity pattern against all pathogens studied i.e. AgNPs > CuONPs > AlONPs > FeONPs. Tested nanoparticles registered lower MIC values and more intensified growth inhibition against Gram-negative bacteria compared to their Gram-positive counterparts. These results pointed out that the M. oleifera mediated nanoparticles can be prospectivelyutilized in the development of alternative antimicrobials against diverse bacterial infections.

Metal-Based Green Synthesized Nanoparticles: Boon for Sustainable Agriculture and Food Security.

The applications of metal-based nanoparticles (MNPs) in the sustainable development of agriculture and food security have received greater attention in recent years in the science community. Different biological resources have been employed to replace harmful chemicals to reduce metal salts and stabilize MNPs, i.e., green methods for the synthesis have paid attention to the nanobiotechnological advances. This review mainly focused on the applications of green synthesized MNPs for the agriculture sector and food security. Because of the novel domains, the green synthesized MNPs could be helpful in the different areas of agriculture like plant growth promotion, plant disease, and insect/pest management, fungicidal agent, in food security for food packaging, for increasing the shelf life and protection from spoilage, and other purposes. The global scenario of the recent studies on the applications of green synthesized MNPs, particularly in sustainable agriculture and food security, is comprehensively discussed.

Nephroprotective effect of Bryophyllum pinnatum-mediated silver nanoparticles in ethylene glycol-induced urolithiasis in rat.

A large population is suffering from multifactorial urolithiasis worldwide with a reoccurrence rate of almost 70%-80% in males and 47%-60% in females. In the present study, the nephroprotective effect of silver nanoparticles (AgNPs) synthesised by Bryophyllum pinnatum was evaluated in ethylene glycol-induced urolithiasis in rat. B. pinnatum-mediated AgNPs which were found to be spherical and polydispersed particles with an average size of 32.65 nm determined by transmission electron microscopy analysis, and showing an absorption peak at 432 nm by the UV-Vis spectrophotometric analysis, revealing the role of hydroxyl group in the synthesis by Fourier Transformed Infrared Spectroscopy analysis, with a zeta potential value of -15.7 mV. The crystalline nature and fcc structure was demonstrated based on X-ray diffraction analysis. Animal study was performed on 36 male Wistar rats divided into six equal groups, which demonstrated significant increase in serum total protein, albumin and globulin and significant decrease in AST, ALT, creatinine, BUN, calcium and phosphorus in group V and VI when compared with group II and IV. No crystalluria was observed in rats given B. pinnatum AgNPs. Histopathological observations in group V and VI showed mild degenerative changes and restoration or maintenance of kidney parenchyma when compared with group II and IV rats. Thus, the authors conclude with the beneficial preventive and therapeutic nephroprotective effect of B. pinnatum-mediated AgNPs against ethylene glycol-induced urolithiasis in rats.

Nanotechnology as a Shield against COVID-19: Current Advancement and Limitations.

The coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global health problem that the WHO declared a pandemic. COVID-19 has resulted in a worldwide lockdown and threatened to topple the global economy. The mortality of COVID-19 is comparatively low compared with previous SARS outbreaks, but the rate of spread of the disease and its morbidity is alarming. This virus can be transmitted human-to-human through droplets and close contact, and people of all ages are susceptible to this virus. With the advancements in nanotechnology, their remarkable properties, including their ability to amplify signal, can be used for the development of nanobiosensors and nanoimaging techniques that can be used for early-stage detection along with other diagnostic tools. Nano-based protection equipment and disinfecting agents can provide much-needed protection against SARS-CoV-2. Moreover, nanoparticles can serve as a carrier for antigens or as an adjuvant, thereby making way for the development of a new generation of vaccines. The present review elaborates the role of nanotechnology-based tactics used for the detection, diagnosis, protection, and treatment of COVID-19 caused by the SARS-CoV-2 virus.

Fusarium as a Novel Fungus for the Synthesis of Nanoparticles: Mechanism and Applications.

Nanotechnology is a new and developing branch that has revolutionized the world by its applications in various fields including medicine and agriculture. In nanotechnology, nanoparticles play an important role in diagnostics, drug delivery, and therapy. The synthesis of nanoparticles by fungi is a novel, cost-effective and eco-friendly approach. Among fungi, Fusarium spp. play an important role in the synthesis of nanoparticles and can be considered as a nanofactory for the fabrication of nanoparticles. The synthesis of silver nanoparticles (AgNPs) from Fusarium, its mechanism and applications are discussed in this review. The synthesis of nanoparticles from Fusarium is the biogenic and green approach. Fusaria are found to be a versatile biological system with the ability to synthesize nanoparticles extracellularly. Different species of Fusaria have the potential to synthesise nanoparticles. Among these, F. oxysporum has demonstrated a high potential for the synthesis of AgNPs. It is hypothesised that NADH-dependent nitrate reductase enzyme secreted by F. oxysporum is responsible for the reduction of aqueous silver ions into AgNPs. The toxicity of nanoparticles depends upon the shape, size, surface charge, and the concentration used. The nanoparticles synthesised by different species of Fusaria can be used in medicine and agriculture.

Myco-Fabrication of Copper Nanoparticles and Its Effect on Crop Pathogenic Fungi.

Phytopathogens are responsible for huge losses in the agriculture sector. Amongst them, fungal phytopathogen is quite difficult to control. Many chemicals are available in the market, claiming the high activity against them. However, the development of resistance by the fungal pathogen is the main concern to overcome their menace. Nanotechnology-based products can be a potential alternative to conventional fungicides. Amongst various nanoparticles, Copper nanoparticles (CuNPs) are appearing to be a promising antifungal candidate. It can be synthesized by various methods, but the myco-fabrication appears to be an environmental-friendly approach. Hence, the present study is an attempt to synthesize CuNPs using Aspergillus flavus. The myco-fabricated CuNPs were characterized by UV spectrophotometer, Fourier transform infrared spectroscopy (FTIR), Nanoparticles tracking and analysis system (NTA), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD) and Zeta potential measurement. Myco-fabricated CuNPs showed maximum absorbance at 602 nm and particle size ranging 5-12 nm with the least average size of 8 nm with spherical shape and moderate stability. Myco-fabricated CuNPs tested against selected fungal crop pathogens viz. Aspergillus niger, Fusariumoxysporum, and Alternaria alternata reveal a significant effect. Besides these we have given the hypothetical mechanism depicting the antifungal action of myco-fabricated CuNPs.

A novel nano-sulphur and essential oil-based room freshener

Introducción: Alternaria spp y Candida spp. son hongos patógenos de ambientes interiores como la casa, la oficina, el aula, etc., causan enfermedades como asma crónica e infecciones sistémicas en individuos inmunodeprimidos a través de la secreción de diversas sustancias tóxicas. Los ambientadores a base de productos químicos disponibles comercialmente que se utilizan para controlar la carga de hongos en el ambiente interior no son beneficiosos para la salud humana. Objetivo: proporcionar una alternativa viable en forma de enfoque basado en nanopartículas para el manejo de hongos transmitidos por el aire. Metodología: aislamiento, identificación microscópica y bioquímica de hongos de interior; Síntesis de nanopartículas de azufre (SNP) mediadas por Azadirachta indica, su detección y caracterización; y evaluación in vitro de SNP contra hongos aislados presentes en el ambiente interior. Resultado: Los hongos aislados se identificaron como especies de Alternaria spp y Candida spp. Los SNP mostraron máximos de absorbancia a 291 nm. El análisis NTA mostró un tamaño medio de 188,4 nm y un potencial zeta de -4,94 mV, lo que representa una síntesis de SNP estables. El patrón XRD confirmó la naturaleza cristalina cúbica centrada en la cara de los SNP. El espectro FTIR representó la presencia de compuestos polihidroxilo, nitrilo, ceto, aromáticos y carboxílicos que estabilizaron los SNP. Los ensayos antifúngicos demostraron la actividad significativa de los SNP formulados y del ambientador infundido con aceite de eucalipto. Conclusión: Los SNP mediados por A. indica se pueden aplicar en la formulación y fabricación de un ambientador ecológico para el manejo de hongos patógenos de interior como Alternaria spp y Candida spp.

Introduction: Alternaria spp. and Candida spp. are the main fungal pathogen of indoor environment like house, office, classroom, etc. These may cause various diseases and infections like systemic infections, or chronic asthma in immunocompromised individuals through secretion of various toxic substances. Chemical-based commercially available room fresheners used to control the fungal load of indoor environment are not beneficial to human health. Objective: was to provide viable alternative in the form of nanoparticle-based approach for the management of air-borne fungi. Methodology: The present study primarily focuses on the isolation, microscopic and biochemical identification of indoor fungi; Azadirachta indica-mediated sulphur nanoparticles (SNPs) synthesis, their detection and characterization; and in vitro assessment of SNPs against isolated fungi present in indoor environment. Result: The isolated fungi were identified as Alternaria spp and Candida spp. The SNPs showed absorbance maxima at 291 nm. NTA analysis showed average size of 188.4 nm, and zeta potential of -4.94 mV which represented synthesis of stable SNPs. XRD pattern confirmed the face centered cubic, crystalline nature of SNPs. FTIR spectrum depicted the presence of polyhydroxyl, nitrile, keto, aromatic and carboxylic compounds which stabilized the SNPs. The antifungal assays demonstrated the significant activity of the formulated SNPs and eucalyptus oil infused air freshener. Conclusion: It can be concluded that A. indica-mediated SNPs can be applied in the formulation and manufacture of an ecofriendly air freshener for the management of indoor fungal pathogens like Alternaria spp. and Candida spp.

Biogenic Synthesis of Zinc Oxide Nanoparticles by Bryophyllum pinnatum and its Acute Oral Toxicity Evaluation in Wistar Rats.

The evaluation of toxic effects of nanoparticles (NPs) has become an important aspect of Nanotechnology research in the 21st century. The present investigation deals with the green synthesis of biogenic zinc oxide nanoparticles (ZnO-NPs) using Bryophyllum pinnatum leaves, their characterization and evaluation of acute oral toxicity in Wistar rats. The characterization of synthesized ZnO-NPs revealed maximum absorbance at 307 nm on UV-Vis spectrophotometric analysis, NTA showed mean size of particles and mode of the particles distribution as 128.2 nm and 12.6 nm, respectively. Zeta potential was found to be -0.369 mV. The absorbance shown by FTIR at 3469, 1644, 1355 and 887 cm-1 indicates the involvement of biomolecules that are accountable for capping and stabilization of ZnO-NPs. The XRD assessment further demonstrated the crystalline nature of the ZnO-NP. The TEM analysis of the synthesized ZnO-NPs revealed the presence of spherical NPs with the mean size of 3.7 nm. The acute oral toxicity evaluation in rat showed an approximate median lethal dose to be more than 2000 mg/kg body weight. It is thus concluded that biogenic ZnO-NPs showed absence of acute oral toxicity symptoms at the doses employed in the present study.

Novel nanoplex-mediated plant transformation approach.

Here, a rapid and easy transformation by electroporation technique for gene transfer in plants using cell penetrating amino nanocomplex (nanoplex) has been demonstrated in Nicotiana. Nanoplex was prepared using cell penetrating amino acids (CPAs) such as poly-L-lysine (PLL) and Argenine (Arg), in combination with the gold nanoparticles (AuNPs). PLLs-modified nanoplex with zeta potential of 34.2 ± 1.22 mV charge showed 63.3% efficiency for gene transformation in plant cells as compared to 60% when modified with Arg and the zeta potential was found to be 30.0 ± 0.83 mV; whereas, the transformation efficiency without nanoplex was found to be 6.6%. The findings indicate that the zeta potential of positively charged nanocomplex (AuNPs/CPAs/DNA/CPAs) increases the transformation efficiency because of their ability to protect the DNA from electroporation wave and endogenous enzyme damage. Transformation was confirmed by GUS assay and amplification of npt gene. This technique may open up new possibilities of gene transfer in plants, which will enable to produce large number of transgenic plants.

Marine-derived Phoma-the gold mine of bioactive compounds.

The genus Phoma contains several species ubiquitously present in soil, water, and environment. There are two major groups of Phoma, viz., terrestrial and marine. After 1981 researchers all over the world have focused on marine-derived Phoma for their bioactive compounds. The marine Phoma are very rich sources for novel bioactive secondary metabolites, which could potentially be used as drugs. Recently, a large number of structurally unique metabolites with potential biological and pharmacological activities have been isolated from the marine Phoma species particularly Phoma herbarum, P. sorghina, and P. tropica. These metabolites mainly include diterpenes, enolides, lactones, quinine, phthalate, and anthraquinone. Most of these compounds possess antimicrobial, anticancer, radical scavenging, and cytotoxic properties. The present review has been focused on the general background of Phoma, current approaches used for its identification and their limitations, difference between terrestrial and marine Phoma species. In addition, this review summarizes the novel bioactive compounds derived from marine Phoma and their biological activities.