Diagnostic performance: a comparative study of the leukocyte differential count on four automated haematology analysers.

The diagnostic performance of the haematology analysers, Technicon H1, Sysmex NE 8000, Coulter STKS and Sequoia Cell Dyn 3000 was determined by comparing their results for automated differential counting with the result obtained for 400 cells by the manual microscopic method. These comparative studies were performed on a group of adults, containing both normal individuals (n = 150) and those affected by various pathologies (n = 113). The number of morphologically false negatives is low for all the systems (from 1.8% for the Cell Dyn 3000 to 6.2% for the NE 8000), while the number of distributionally false negatives was slightly higher (from 4.4% for the Cell Dyn 3000 to 7% for the H1 and NE 8000). The morphological flags, though useful for improving the diagnostic performance of the instruments, show a rather modest sensitivity when taken individually: immature granulocytes/bands from 71% for the STKS to 43% for the NE 8000; blasts from 66.6% for the H1 to 53.3% for the STKS, atypical lymphocytes from 59% for the H1 to 13.6% for the NE 8000; erythroblasts from 42.8% for the STKS to 7% for the NE 8000.

Evaluation of four automated hematology analyzers. A comparative study of differential counts (imprecision and inaccuracy).

The authors evaluated the performance of four modern, commercially available hematology analyzers for imprecision and inaccuracy in determining the leukocyte differential count. The evaluation was performed according to International Committee for Standardization in Haematology protocols and the National Committee for Clinical Laboratory Standards H20-T standard, using the same group of patients simultaneously. Imprecision was very low among all the analyzers for neutrophils and lymphocytes (coefficient of variation maximum = 4.12%), whereas for the other leukocyte populations it tended to increase as their presence percentage decreased. The imprecision of the analyzers was still lower than that of the microscopic method. The correlation with the manual 800 cell count (inaccuracy) was good for neutrophils, lymphocytes, and eosinophils (r = 0.974 to 0.888), less so for monocytes (r = 0.757 to 0.490), whereas it was poor for basophils (r = 0.532 to 0.078).

[Incidence of salmonellosis in the Province of Trento in the past 3 years]. / Incidenza delle salmonellosi in provincia di Trento nell'ultimo triennio.

72 strains of Salmonella, belonging to 23 different serum types have been isolated during the last three years. These strains come from adult patients admitted to the Infective department of St. Chiara hospital in Trento and from three asymptomatic carriers found among the hospital staff, who have been regularly submitted to microbiological checks. Among the most frequent strains the AA. have found: S. enteritidis and S. typhi murium. Salmonellas, never isolated before, have spread, but they have shown poor adaptation to the environment: among these S. panama, S. infantis, S infantis, S. münchen. S. wien has not found, in the Province of Trento, environing conditions fit for its colonization. S. agona has shown a certain tendency to create a situation of asymptomatic carrier.