ABSTRACT
The structure of the actinic cytoskeleton in Vero cells (African green monkey kidney cells) during monolayer formation was studied. By immunofluorescence after phalloidin staining, actin was visualized in cells 15 min, 2, 4, 24 and 48 hours after cultivation. The evolution in time of cells revealed the progressive organization of actin molecules from diffuse granular forms to filamentous forms with characteristic patterns. The evolution of actinic cytoskeletal elements was closely correlated with the evolution of the cell shape and the extension of intercellular contact surfaces.
Subject(s)
Actin Cytoskeleton/ultrastructure , Actins/ultrastructure , Vero Cells/ultrastructure , Animals , Chlorocebus aethiops , Microscopy, Fluorescence , Time FactorsABSTRACT
The evolution of the actin cytoskeleton after trypsinization and recultivation as well as the effect of the PGE2 modulator and that of the secondary messenger, the cyclic AMP upon the same cytoskeletal proteins in human pulmonary fibroblasts (ICP-23) were studied. The substances were administered simultaneously and after one hour of viral adsorption. Using epifluorescence for pointing out filamentous actin the modifications occurring in this cytoskeletal protein when contacting trypsin and the virus and when PGE2 and cAMP are administered in the experimental variants are observed. Actin arrangement is obviously modified by the viral infection but the restrictive effect of PGE2 and cAMP upon virus replication is correlated with modifications occurring in the actin cytoskeleton.
