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Histochemistry ; 90(3): 215-7, 1988.
Article in English | MEDLINE | ID: mdl-3215793

ABSTRACT

Two cytochemical methods for the localization of acid and alkaline invertases are given. The first is based upon the reduction of a silver complex at two different pH ranges, whilst the second is based upon the tetrazolium reaction and permits quantification of the rate of activity of alkaline invertase activity. The distribution of alkaline invertase activity throughout the root apex of Pisum sativum and the cell wall localization of acid invertase for material excised from tuber tissue of Helianthus tuberosus are both confirmed.


Subject(s)
Glycoside Hydrolases/analysis , Histocytochemistry/methods , Plants/enzymology , Nitroblue Tetrazolium , Silver Nitrate , beta-Fructofuranosidase
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