ABSTRACT
Due to the negative autopsy and without cardiac structural abnormalities, unexpected sudden cardiac death ï¼USCDï¼ is always a tough issue for forensic pathological expertise. USCD may be associated with parts of fatal arrhythmic diseases. These arrhythmic diseases may be caused by disorders of cardiac ion channels or channel-related proteins. Caveolin can combine with multiple myocardial ion channel proteins through its scaffolding regions and plays an important role in maintaining the depolarization and repolarization of cardiac action potential. When the structure and function of caveolin are affected by gene mutations or abnormal protein expression, the functions of the regulated ion channels are correspondingly impaired, which leads to the occurrence of multiple channelopathies, arrhythmia or even sudden cardiac death. It is important to study the effects of caveolin on the functions of ion channels for exploring the mechanisms of malignant arrhythmia and sudden cardiac death.
Subject(s)
Arrhythmias, Cardiac/physiopathology , Caveolins/metabolism , Channelopathies/genetics , Ion Channels/metabolism , Arrhythmias, Cardiac/genetics , Arrhythmias, Cardiac/metabolism , Autopsy , Channelopathies/complications , Death, Sudden, Cardiac/etiology , Death, Sudden, Cardiac/pathology , Forensic Pathology , Humans , Ion Channels/genetics , Mutation , MyocardiumABSTRACT
OBJECTIVES: To explore the genetic variation sites of caveolin ï¼CAVï¼ and their correlation with sudden unexplained death ï¼SUDï¼. METHODS: The blood samples were collected from SUD group ï¼71 casesï¼, coronary artery disease ï¼CADï¼ group ï¼62 casesï¼ and control group ï¼60 casesï¼, respectively. The genome DNA were extracted and sequencing was performed directly by amplifying gene coding region and exon-intron splicing region of CAV1 and CAV3 using PCR. The type of heritable variation of CVA was confirmed and statistical analysis was performed. RESULTS: A total of 4 variation sites that maybe significative were identified in SUD group, and two were newfound which were CAV1ï¼ c.45C>T ï¼T15Tï¼ and CAV1ï¼c.512G>A ï¼R171Hï¼, and two were SNP loci which were CAV1ï¼c.246C>T ï¼rs35242077ï¼ and CAV3ï¼c.99C>T ï¼rs1008642ï¼ and had significant difference ï¼P<0.05ï¼ in allele and genotype frequencies between SUD and control groups. Forementioned variation sites were not found in CAD group. CONCLUSIONS: The variants of CAV1 and CAV3 may be correlated with a part of SUD group.