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Bing Du Xue Bao ; 24(4): 312-6, 2008 Jul.
Article in Chinese | MEDLINE | ID: mdl-18780636

ABSTRACT

A coxsackievirus B strain was successfully isolated by cells culture from cardiac muscle tissues of a dead Sichuan golden monkey with myocarditis from a zoo of Changchun in China. The isolate was consistent with CVB by morphology, physicochemistry test, animal regression test and RT-PCR. Analysis of VP1 partial gene sequence and detection of mice specific serum IgG showed that the strain isolated was a coxsackievirus B3. It was the first CVB case report in Sichuan golden monkey and the strain isolated was named CVB/SGM-05.


Subject(s)
Enterovirus B, Human/isolation & purification , Haplorhini/virology , Animals , Chlorocebus aethiops , Heart/virology , Mice , Reverse Transcriptase Polymerase Chain Reaction , Vero Cells , Viral Structural Proteins/genetics
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