ABSTRACT
The cultural, physiologo-biochemical adhesive and antagonistic properties of B. subtilis strains with good prospects for use as biotherapeutic preparations were studied. For further studies B. subtilis strain No. 1719 was chosen. In experiments on non-inbred white mice the animals were treated by the preparation Cifran used for their selective decontamination from opportunistic microflora and for the creation of the state of dysbiosis. The influence of the spore-forming microbe on the parietal microflora of the large intestine of the animals was shown. Reliable data on the changes in the number of microorganisms (CFU/ml) per 1 cm of the surface of the large intestine were established. As markers making it possible to evaluate the action of biotherapeutic and other medicinal remedies, easily determinable ratios of lac+/lac- of bacteria and Staphylococcus aureus/Staphylococcus spp. was proposed.
Subject(s)
Bacillus subtilis/physiology , Fluoroquinolones , Intestinal Diseases/therapy , Intestine, Large/microbiology , Probiotics/therapeutic use , Animals , Antibiosis , Bacillus subtilis/isolation & purification , Bacteria/genetics , Bacteria/isolation & purification , Bacterial Adhesion , Ciprofloxacin/analogs & derivatives , Colony Count, Microbial , Disease Models, Animal , Drug Evaluation, Preclinical , Humans , Intestinal Diseases/chemically induced , Intestinal Diseases/microbiology , Lac Operon , Mice , Staphylococcaceae/isolation & purificationABSTRACT
Data on probiotic preparations prepared from spore-forming bacteria is reviewed. Information on indications for use, effectiveness and the mechanism of action is given. Preparations being produced in Russia (Biosporin, Sporobacterin and Bactosporin) are evaluated in detail as well as the prospects of the application of probiotics from spore-forming bacteria.
