ABSTRACT
AIM: In this study, the suitability of a mixture containing riboflavin (vitamin B2) and triethanolamine (TEOHA) as a novel biocompatible photoinitiator for two-photon polymerization (2PP) processing was investigated. MATERIALS & METHODS: Polyethylene glycol diacrylate was crosslinked using Irgacure(®) 369, Irgacure 2959 or a riboflavin-TEOHA mixture; biocompatibility of the photopolymer extract solutions was subsequently assessed via endothelial cell proliferation assay, endothelial cell viability assay and single-cell gel electrophoresis (comet) assay. Use of a riboflavin-TEOHA mixture as a photoinitiator for 2PP processing of a tissue engineering scaffold and subsequent seeding of this scaffold with GM-7373 bovine aortic endothelial cells was also demonstrated. RESULTS: The riboflavin-TEOHA mixture was found to produce much more biocompatible scaffolds than those produced with Irgacure 369 or Irgacure 2959. CONCLUSION: The results suggest that riboflavin is a promising component of photoinitiators for 2PP fabrication of tissue engineering scaffolds and other medically relevant structures (e.g., biomicroelectromechanical systems).
Subject(s)
Ethanolamines/chemistry , Photoinitiators, Dental/chemistry , Photons , Polyethylene Glycols/chemistry , Polymerization , Riboflavin/chemistry , Water/chemistry , Animals , Aorta/cytology , Biocompatible Materials/pharmacology , Cattle , Cell Proliferation/drug effects , Cell Survival/drug effects , Comet Assay , Endothelial Cells/cytology , Endothelial Cells/drug effects , Ethanolamines/pharmacology , Fluoresceins/metabolism , Materials Testing , Microscopy, Electron, Scanning , Polyethylene Glycols/pharmacology , Riboflavin/pharmacology , Solubility , Spectrophotometry, Ultraviolet , Tissue Scaffolds/chemistryABSTRACT
Two-photon polymerization has developed as a powerful tool for making micro- and nanoscale structures for regenerative medicine applications. This review discusses micro- and nanoscale aspects of tissue engineering, which are followed by a brief description of the two-photon polymerization process and how it has been used thus far in tissue engineering and other regenerative medicine applications. Lastly, potential future applications of two-photon polymerization in regenerative medicine are presented. This review provides a comprehensive summary of the uses of two-photon polymerization thus far in regenerative medicine and a look into how this technique will be used in the future.
Subject(s)
Nanostructures , Photons/therapeutic use , Polymerization , Regenerative Medicine/methods , Tissue Engineering/methods , Tissue Scaffolds/chemistry , Humans , Regenerative Medicine/trendsABSTRACT
Two-photon polymerization technology has been used to fabricate submicrometer three-dimensional (3D) structures using a new polyfunctional perfluoropolyether-based resist, which is a polymer intrinsically hydrophobic and chemically resistant. The fluorinated resist was designed and synthesized in this work and successfully employed to fabricate woodpile structures in various experimental conditions. This is the first demonstration of the capability to fabricate hydrophobic and chemically resistant 3D structures with submicrometer resolution and arbitrary geometry.
ABSTRACT
Application of the two-photon polymerization (2PP) technique for the fabrication of binary radial diffractive optical elements (DOEs) to form a bottle-like intensity distribution, or "light bottle," is studied. Computer modeling and fabrication of a binary DOE for the formation of the desired light distributions are realized. The results of scanning electron microscopy analysis of the diffractive relief produced by the 2PP technique and an investigation of the optical properties of the fabricated elements are presented.
ABSTRACT
Two-photon polymerization has been employed to fabricate three-dimensional structures using the biodegradable triblock copolymer poly(epsilon-caprolactone-co-trimethylenecarbonate)-b-poly(ethylene glycol)-b-poly(epsilon-caprolactone-co-trimethylenecarbonate) with 4,4'-bis(diethylamino)benzophenone as the photoinitiator. The fabricated structures were of good quality and had four micron resolution. Initial cytotoxicity tests show that the material does not affect cell proliferation. These studies demonstrate the potential of two-photon polymerization as a technology for the fabrication of biodegradable scaffolds for tissue engineering.
