[Clinical significance of erythrocyte phosphoenolpyruvate in elderly and senior patients with iron deficiency anemia].

The article presents a part of the study dealing with erythrocyte glycolysis observed in 29 elderly and senior patients with iron deficiency anemia. The accumulation of phosphoenolpyruvate which is high-energy product of glycolysis testifies that erythrocyte glycolytic reactions are further enhanced. The change in phosphoenolpyruvate content may reflect the restoration and normalization of erythropoiesis and testify the appearance of new forms of erythrocytes, in particular, reticulocytes with natural type of glycolysis and energy metabolism. Clinical significance of obtained data is discussed in the article.

[Clinical and hematological aspects of follicular lymphoma]. / Kliniko-gematologicheskie osobennosti follikuliarnoi limfomy.

A comprehensive clinical-and-laboratory examination of patients with non-Hodgkin's malignant lymphomas revealed a lack of correspondence between results of histological and immunophenotypical investigations. The most characteristic feature appeared to be the presence of CD10, CD11a, and CD35 antigens. Absence of CD95 antigen on tumour cells is regarded as an unfavourable prognostic sign in patients with follicular lymphomas as is presence of CD10 antigen on the above cells.

[The histamine and serotonin levels in the blood plasma of diabetic patients]. / Soderzhanie gistamina i serotonina v plazme krovi bol'nykh sakharnym diabetom.

Fluorometry was done in 52 patients with diabetes mellitus to determine the blood plasma content of free histamine and free serotonin. The control group was 29 essentially healthy subjects. Significant increase was found out in free histamine during the stage of decompensation of diabetes mellitus (P < 0.01). With compensation of diabetes mellitus setting in, the content of histamine returns to normal (P < 0.01). The blood plasma level of free histamine can be used as an additional criterion in the assessment of compensation of diabetes mellitus. Free histamine is supposed to play an active part in the pathogenesis of diabetic angiopathies.

[The efficacy of using thymalin and plasmapheresis in the combined treatment of patients with chronic lympholeukemia]. / Effektivnost' primeneniia timalina i plazmafereza v kompleksnom lechenii bol'nykh khronicheskim limfoleikozom.

Results are submitted of study of the effectiveness of inclusion into the treatment program for patients with chronic lymphoid leukemia of specific (thymalin) and unspecific (plasmapheresis) means and modes of immune correction, which made for more rapid attaining clinical and hematological compensation by comparison with chemotherapeutic agents only. Furthermore, the method of combined use of drugs, attempted for the first time, permitted the functional activity of the lymphoid system to be improved, with the clinical and hematological compensation being achieved in a shorter space of time.

[The level of free histamine and free serotonin in the blood plasma in polycythemia vera]. / Soderzhanie svobodnogo gistamina i svobodnogo serotonina v plazme krovi pri istinnoi politsitemii.

Fluorimetric analysis was performed of blood plasma content of free histamine and free serotonin in 26 patients with polycythemia vera (PV) and 29 essentially healthy individuals. There has been established an elevation of blood plasma free histamine and free serotonin concentration in PV patients (p) as compared with those in healthy subjects. The ratio of free histamine: free serotonin in PV patients' blood plasma has gotten significantly decreased (p) as compared with the healthy individuals. The above findings might be regarded as reflecting metabolic disorders due to retardation of bloodflow and increase in viscosity of blood caused by augmentation of the mass of circulating erythrocytes in PV.

[Immunological deficiency in patients with the myeloid forms of acute leukemia and the methods for its correction]. / Immunologicheskaia nedostatochnost' u bol'nykh s mieloidnymi formami ostrogo leikoza i metody ee korrektsii.

Immunologic and cytochemical studies were done in 162 patients with myeloid forms of acute leukosis, among whom 130 had acute myeloblastic leukemia (AML), and thirty two--and acute myelomonoblastic leukemia (AMML), before and after the therapy instituted. Immunocorrection was attempted with immunomodulating agents prodigiosane and tactivin. Striking inhibition of T-cellular immunity (T-total, T-active, TPhres, TPhsen, as well as blast transformation of lymphocytes with PHA) was revealed. All patients experienced significant rise in circulating immune complexes before treatment. Investigation designed to study intracellular metabolism of neutrophils demonstrated significant decline in the activities of G-6-P-ase, NAD- and NADP-oxidases, cationic proteins and NBT-test. Immunocorrective agents improve the state of immunologic reactivity, make for arresting infections and inflammatory processes and prolongation of remission in patients with acute myeloid forms of leukoses.

[Free histamine and free serotonin in the blood plasma in symptomatic respiratory erythrocytosis]. / Svobodnyi gistamin i svobodnyi serotonin v plazme krovi pri simptomaticheskom dykhatel'nom éritrotsitoze.

Fluorometric analysis was carried out of the blood plasma content of free histamin (FH) and free serotonin (FS) in 27 patients with chronic non-obstructive bronchitis (ChNB), 52 patients with chronic obstructive bronchitis (ChOB) presenting with symptomatic erythrocytosis (SE) and in 29 normal subjects. Comparative analysis of the results obtained showed that in SE caused by ChOB the FH and FS content is significantly higher (p < 0.001) than in ChNB and in normal subjects. The FH content increases in SE with the augmentation in erythrocytosis. Positive dynamics was noted of FH and FS during the course of the disease treatment (p < 0.001).

[Biogenic amines in the differential diagnosis of erythrocytes]. / Biogennye aminy v differentsial'noi diagnostike éritrotsitov.

Free histamine (FH) and free serotonin (FS) were studied fluorometrically in blood serum of 26 patients with genuine polycythemia (GP), 52 patients with chronic obstructive bronchitis (COB) associated with secondary erythrocytosis (SE) and 29 healthy subjects. On the grounds of the results of the study criteria of GP and COB-related SE were suggested. Diagnosis of GP can be considered valid if content of FH is no more than 3.1 times, FS content no less than 3 times and FH/FS ratio is no more than 2.65 times as much as normal. Levels of FH and FS and FH/FS ratio in SE related to COB are characterized by 4-fold and more, 2.9 and less and 4.64 and more increase respectively.

[Disordered trace element content of the erythrocytes in diabetes mellitus]. / Narushenie soderzhaniia mikroélementov v éritrotsitakh pri sakharnom diabete.

Content of chemical elements (CE) in washed out erythrocytes was studied by nuclear-absorptive spectroscopy in 26 patients with insulin-dependent diabetes mellitus (IDDM), 26 patients with non-insulin-dependent diabetes mellitus (NIDDM) and 29 healthy subjects. It was established that erythrocytes in IDDM contain significantly less amounts of cuprum, manganese and cobalt than in healthy subjects, content of zinc being increased. Contents of iron, cuprum, manganese and zinc were found to be decreased in patients with NIDDM as compared to the control. During the conventional treatment erythrocytes of patients with IDDM revealed increase of cuprum content while in NIDDM rise in amounts of iron, manganese, cobalt and zinc was observed. Changes of erythrocytes CE content in diabetes mellitus are supposed to be of compensatory-adaptive nature, for decompensation of IDDM and NIDDM is associated with more pronounced CE dysbalance. Increased erythrocyte content of zinc in IDDM may be explained by uptake of prolonged insulin used for substituting treatment.

[The integral tetrazolium activity of the neutrophils in patients with chronic lympholeukemia]. / Integral'naia tetrazolievaia aktivnost' neitrofilov u bol'nykh khronicheskim limfoleikozom.

A study is presented of the functional activity of peripheral blood neutrophils in 58 patients with chronic lympholeucosis, 27 patients with purulent-inflammatory diseases of the nasopharynx. Patients with chronic lympholeucosis were subdivided in two groups: with infectious complications and without these complications. The reaction of reduction nitroblue tetrazolium was used (Park method in modification by Shubich and Mednikova). The integral tetrazolium activity of neutrophils was determined according to a formula proposed by Drannik and Kovalev. Results indicate that determination of the functional activity of neutrophils has a high information value, allows to evaluate the potentialities of the immune system of the body and plan ways of correction.

[A decrease in the activity of granulocyte cationic protein in patients with chronic lympholeukemia]. / Snizhenie aktivnosti kationnogo belka granulotsitov u bol'nykh khronicheskim limfoleikozom.

A study of 60 patients suffering chronic lympholeukemia indicates disorders of the functional adequacy of neutrophils that shows further deterioration with association of infection (pneumonia, angina, abscesses and other infections). By the content of cationic protein of the neutrophils it becomes possible to evaluate the state of unspecific defense of the body and severity of the pathological process. The cationic lysosomal test may be recommended for the diagnosis of infectious complications in hemoblastoses.

[Differential diagnostic criteria of blast cells in the peripheral blood in acute myeloid leukemias]. / Differentsial'no-diagnosticheskie kriterii blastnykh kletok perifericheskoi krovi pri ostrykh mieloidnykh leikozakh.

The authors present their data on results of an investigation of blast cells including morphocytochemical examination, determination of the presence of receptors to Fc-fragments of Ig, to C3 component of the complement. Criteria were obtained permitting to differentiate myeloid forms of acute leukosis.

[Myeloperoxidase activity of neutrophils and the frequency of emergence of infectious complications in myeloproliferative disorders]. / Aktivnost' mieloperoksidazy neitrofilov i chastota vozniknoveniia infektsionnykh oslozhnenii pri mieloproliferativnykh zavolevaniiakh.

Myeloperoxidase activity in segmented neutrophils has been assessed in peripheral blood of 122 patients with myeloproliferative disorders (MPD). For each MPD entity there appeared a definite relationship between hypoactivity of the enzyme and enhanced incidence of infectious complications.