[The development of complex technique of evaluation of virulence of parahemolytic vibrio].

The article deals with results of studying parahemolytic vibrio separatedfrom different sources according their phenotype and genotype attributes associated with virulence. In certain cases the mismatch of results of Kanagava tests and polymerase chain reaction test of gene tdh was established. The need in virulence complex evaluation is substantiated. This complex has to include detection of hemolytic activity in Kanagava test and urease activity on the Kristensen medium and polymerase chain reaction detection of genes tdh and trh. The developed complex technique is described. The formula of pathogenic strains is established Three alternatives of virulent parahemolytic vibrio are given. The test-strains Vibrio parahaemolyticus are proposed as control in testing phenotype and genotype strains according virulence signs.

[A case of Crimean-Congo hemorrhagic fever in the Anapa District, Krasnodar Territory].

The paper gives information on the first case of Crimean-Congo hemorrhagic fever detected in a female resident of the Anapa District, Krasnodar Territory, in 2005 in the past 57 years.

[Biological and genetic characterization of strains of the pathogen of legionellosis, isolated from water on Russian territory]. / Biologicheskie i geneticheskie kharakteristiki shtammov vozvuditelia legionelleza, vydelennykh iz vody na territorii Rossii.

As the result of the study of the spread of Legionella in different regions of Russia, 69 cultures were isolated from different water systems. After serotyping most of these strains (85%) were identified as L. pneumophila, serogroups 1 and 6. 57% of the isolated Legionella strains were shown to be capable of causing fatal infection in guinea pigs, but only 50% of virulent cultures had a high level of virulence. More than a half of the isolated strains (67%) contained plasmid DNA with mol. weights ranging from 2.5 to 80 mD. In some strains of serogroup 1 the identity of plasmids was established by restriction analysis (endonucleases Hind III and Bam HI). The data thus obtained are indicative of the potential epidemic danger of L.pneumophila strains circulating in the environment.

[The design of bacteriological preparations for the diagnosis of legionellosis]. / Konstruirovanie bakteriologicheskikh preparatov dlia diagnostiki legionelleza.

The technique of making legionellosis diagnostic preparations is described, making use of traditional methods and new modified procedures for isolation and purification of the components of serological biosystems. Results of using bacteriological preparations in studies of legionellosis are presented.

[Phages of halophilic vibrios]. / Fagi galofil'nykh vibrionov.

The morphology and antigenic properties of 34 phages of Vibrio parahaemolyticus and V. alginolyticus strains isolated from Black Sea water, as well as their range of lytic activity and specificity of action, have been studied. Most of these phages have been found to lyze both V. parahaemolyticus and V. alginolyticus strains. In addition, highly specific phages capable of lyzing only V. parahaemolyticus serotype O5:K15, have been detected. The latter fact is a precedent indicating the possibility of search for other typing phages and the development of the scheme for the phage typing of V. parahaemolyticus in future.

[The fatty acid composition of the total lipids in Vibrio cholerae]. / Zhirnokislotnyi sostav summarnykh lipidov Vibrio cholerae.

The fatty acid composition of total cell lipids of V. albensis typing strains, serovars 01-012, isolated from patients and environmental objects, as well as V. eltor and NAG vibrios, has been studied. Fatty acids contain 14-18 carbon atoms and are mainly represented by unsaturated monoacids. Palmitoleic and oleic acids constitute the greater part of unsaturated acids (their total content is 55.2-71.0%). The level of saturated fatty acids in considerably lower (27.0-43.1%), and these acids are mainly represented by palmitic acid, its maximum level being 28.4%. The similarity of the fatty acid profiles of the lipids common to the strains under study may indicate that these strains are phylogenetically related.

[The lipids of phosphorescent Vibrio albensis bacteria]. / Lipidy svetiashchikhsia bakterii Vibrio albensis.

The lipid composition of fluorescent vibrios, V. eltor and nonagglutinating vibrios has been studied. In the fraction of polar lipids phosphatidylethanolamine, phosphatidylinositol and cardiolipin and in the fraction of neutral lipids monoglycerides, free fatty acids, diglycerides, triglycerides, sterol esters have been identified. The fatty acid composition of some classes of neutral lipids have been determined. Both similarity and differences between the strains under study in their lipid and fatty acid composition have been established.

[The isolation of Vibrio fluvialis on the territory of the USSR]. / O vydelenii Vibrio fluvialis na territorii SSSR.

For the first time V. fluvialis strains were detected on the territory of the USSR. The taxonomic position of these vibrios was determined by their nucleotide DNA composition (the content of guanine + cytosine was 49.3-51.0 mole%) and the characteristic features of their phenotype. The individual features of the strains consisted in their capacity for agglutination with cholera antisera, groups 01 and Inaba, in diagnostic dilutions in the presence of differences in genomes and phenotypes with cholera vibrios. Molecular hybridization DNA-DNA also gave no confirmation of their relationship to cholera vibrios (23-26% homology). The comparative study of V. fluvialis strains from the USSR and other countries by a broader set of their phenotypical signs confirmed their identity.

Serological diagnosis of diarrhoea caused by some representatives of the genera Vibrio and Aeromonas.

Various serological methods were used to study 630 blood sera of patients with diarrhoea on whom microorganisms of the genera Vibrio (vibrios of non-01 group, phosphorescent, parahaemolytic and alginolytic vibrios) and Aeromonas were isolated, and also of patients with unconfirmed bacteriological diagnosis, carriers and healthy persons. It has been established that specific antibodies are isolated in 30-50% of cases, most frequently in the agglutination reaction, in PHAR with flagellar erythrocyte diagnostic agent, in the reaction of vibriocidal antibodies using the Takachi apparatus. Serological diagnosis is a subsidiary method promoting the performance of clinico-epidemiological analysis of diseases with diarrhoea.

[Taxonomic position of representatives of the species Vibrio cholerae]. / O taksonomicheskom polozhenii nekotorykh predstavitelei vida Vibrio cholerae.

The data contained in the works of some foreign authors who have reconsidered the taxonomic position of V. metschnikovii are analyzed. In contrast to the views expressed by Gamaleya whi believed this microorganism to be the modified cholera vibrio, the foreign authors identify it with the "Kommabacillus der Cholera nostras" described by Finkler and Prior (1884) and classify it with saprophytes widely spread in the environment. These points of view were refuted by the data resulting from the study of the strains of V. metschnikovii and the Finkler - Prior vibrio from Soviet collections. The variant from the Odessa Institute of Epidemiology and Microbiology has been found to be most similar to the original characteristic given by Gamaleya and should be considered the lectotype of V. metschnikovii.

[Serological typing of Vibrios, nonagglutinating with O-cholera serum, isolated in USSR]. / Serologicheskoe tipirovanie ne aggliutiniruiuschikhsia O-kholernoi syvorotkoi vibriunov, vedelennykh na territorii SSSR.

The authors carried out serological typing of 2008 strains of NAG vibrios isolated in 1968--1975 in 18 territories of the Soviet Union from humans and from the objects of environment. Serological type was established in 40.5% of the srains isolated from humans and in 16% of the strains obtained from the environmental objects. Among persons with acute gastrointestinal diseases serotype 5 was the one which dominated, and among strains from the environment--serotype 8 Sakazaki. Serological type 15 was revealed for the first time in the USSR at the territory of Siberia and the Far East among the NAG-vibrio strains isolated from the environment.

[Role of neuraminidase from cholera vibrions in the pathogenesis of experimental cholera]. / O roli neiraminidazy kholernykh vibrionov v patogeneze éksperimental'noi kholery

A study was made of a possible effect of neuraminidase of cholera vibrios on cholera pathogenesis. It was shown that in intraintestinal injection of cholera vibrios of the El Tor biotype to nursling rabbits neuraminidase could be revealed in their intestine 5 to 8 hours after the infection. Addition of neuraminidase to the weakly cholerogenic strain cholera vibrios intensified its cholerogenic action in infection of the animals. The antineuraminidase serum administered to the infected rabbits prevented clinical manifestations of experimental cholera, although it failed to always eliminate the cholerogenic syndrome (revealed during autopsy). At the same time neuraminidase did not influence the capacity of cholerogen to produce the cholerogenic syndrome. The authors consider that the action of the enzyme should occur at the early stages of the pathogenic process, and could be associated with creation of conditions for the attachement of cholera causative agent to the intestinal wall or for the action of their exotoxin.