ABSTRACT
Accepted methods of the ESR methodology (the Westergren mode and ZSR mode) and its alternative the plasma viscosity were tested for diagnostic utility in pregnancy induced hypertension and pre-eclampsia. The receiver-operating characteristic curve (ROC) analysis approved moderate diagnostic accuracy for the ESR methodology and supplied support for its preliminary estimated cutoff values but failed to indicate cogent discernment of pathology by values of plasma viscosity. Likely pathological whole blood alterations boost the erythrocyte aggregation while the concomitant depletion of macromolecules degrades plasma viscosity values.
Subject(s)
Blood Sedimentation , Blood Viscosity , Hemorheology/methods , Hypertension/blood , Area Under Curve , Arteries/pathology , Erythrocyte Aggregation , Female , Humans , Pre-Eclampsia , Pregnancy , Pregnancy Complications, Cardiovascular , ROC Curve , Regression AnalysisABSTRACT
A probable conjunction of hemodynamic- and rheological variables was tested in hypertensive patients. Most pronounced correlation was fixed for some indices (sizable in value for plasma viscosity and small but distinct for blood pressure and ZSR) vs. total peripheral resistance. These results of a small scale elaboration are encouraging to get up a study extension.
Subject(s)
Blood Viscosity/physiology , Erythrocyte Aggregation/physiology , Hypertension/blood , Hypertension/physiopathology , Vascular Resistance/physiology , Blood Pressure/physiology , Erythrocyte Count , Female , Humans , Leukocyte Count , Middle Aged , Reference ValuesABSTRACT
We have studied the level of lipid peroxidation in the liver of rats exposed to nitrous oxide plus oxygen or injected with droperidol of fentanyl. The effect of nitrous oxide used in combination with droperidol and fentanyl was also investigated. All the tested anaesthetics caused lipid peroxidation in the rat liver. It seems likely, however, that the mechanism by which droperidol and fentanyl initiate lipid peroxidation differs from that which nitrous oxide uses. Free radical products and/or activated oxygen species are produced during fentanyl and droperidol metabolism in the liver. However, nitrous oxide is not metabolized in the liver and probably undergoes one electron reduction outside the liver thus producing free radical products and/or activated oxygen species which are able to diffuse and initiate lipid peroxidation in the liver. It was also found that the level of lipid peroxidation in the liver of rats injected with droperidol and fentanyl and then exposed to nitrous oxide was low and close to that of the control animals. We suggest that, when used in combination, the products generated outside the liver as a result of nitrous oxide metabolism are transported to the liver and take part in reactions with the products of the metabolism of droperidol and fentanyl, thus decreasing the concentration of the species able to initiate lipid peroxidation.
Subject(s)
Droperidol/pharmacology , Fentanyl/pharmacology , Lipid Peroxidation/drug effects , Liver/metabolism , Nitrous Oxide/pharmacology , Animals , Droperidol/administration & dosage , Fentanyl/administration & dosage , Luminescent Measurements , Male , Malondialdehyde/metabolism , Nitrous Oxide/administration & dosage , Rats , Rats, Wistar , Vitamin E/metabolismABSTRACT
The polyamines spermine and spermidine and the diamine putrescine inhibit lipid peroxidation in phospholipid liposome suspensions and rat liver homogenates. Using the chemiluminescence technique the antioxidant activity of polyamines was found to be due to reactions with the free radical intermediates of lipid peroxidation and/or superoxide radicals. Also, the antioxidant action of polyamines correlated with the amount of their amino groups: the antioxidant activity increases from putrescine to spermine.
Subject(s)
Lipid Peroxidation/drug effects , Liver/metabolism , Polyamines/pharmacology , Animals , Iron , Liposomes , Liver/drug effects , Luminescent Measurements , Male , Malondialdehyde/analysis , Phospholipids/metabolism , Polyamines/chemistry , Putrescine/pharmacology , Rats , Rats, Wistar , Spermidine/pharmacology , Spermine/pharmacologyABSTRACT
The dynamics of lipid peroxidation in isoprenaline-induced myocardiopathy was studied in rats. Spontaneous and Fe(II)- catalyzed generation of malondialdehyde (MDA)-like products and chemiluminescence in the heart, liver and brain homogenates were measured. The increase in the interval between treatment of rats with isoprenaline and their killing up to 48 hours led to an increase in MDA content in the heart. Both spontaneous and Fe(II)-induced chemiluminescence also reached their maxima after 48 hours. These data show that well expressed lipid peroxidation in the rat heart occurs approximately 48 hours after isoprenaline application. Isoprenaline metabolization in the liver leads to generation of activated oxygen species which are able to induce lipid peroxidation in the presence of Fe(II). The treatment of rats with isoprenaline caused well-expressed lipid peroxidation in the brain. The maximum of this process occurred approximately 36 hours after isoprenaline application. The results show that both spontaneous and Fe(II)-induced chemiluminescence might be used for the estimation of lipid peroxidation in rat heart homogenates.
