Lipid mediated plant immunity in susceptible and tolerant soybean cultivars in response to Phytophthora sojae colonization and infection.

BACKGROUND: Soybean is one of the most cultivated crops globally and a staple food for much of the world's population. The annual global crop losses due to infection by Phytophthora sojae is currently estimated at $20B USD, yet we have limited understanding of the role of lipid mediators in the adaptative strategies used by the host plant to limit infection. Since root is the initial site of this infection, we examined the infection process in soybean root infected with Phytophthora sojae using scanning electron microscopy to observe the changes in root morphology and a multi-modal lipidomics approach to investigate how soybean cultivars remodel their lipid mediators to successfully limit infection by Phytophthora sojae. RESULTS: The results reveal the presence of elevated biogenic crystals and more severe damaged cells in the root morphology of the infected susceptible cultivar compared to the infected tolerant cultivars. Furthermore, induced accumulation of stigmasterol was observed in the susceptible cultivar whereas, induced accumulation of phospholipids and glycerolipids occurred in tolerant cultivar. CONCLUSION: The altered lipidome reported in this study suggest diacylglycerol and phosphatidic acid mediated lipid signalling impacting phytosterol anabolism appears to be a strategy used by tolerant soybean cultivars to successfully limit infection and colonization by Phytophthora sojae.

Dynamic interplay of metal and metal oxide nanoparticles with plants: Influencing factors, action mechanisms, and assessment of stimulatory and inhibitory effects.

Nanoparticles (NPs) of metals and metal oxides have received increasing attention regarding their characteristic behavior in plant systems. The fate and transport of metal NPs and metal oxide NPs in plants is of emerging concern for researchers because they ultimately become part of the food chain. The widespread use of metal-based NPs (MBNPs) in plants has revealed their beneficial and harmful effects. This review addresses the main factors affecting the uptake, translocation, absorption, bioavailability, toxicity, and accumulation of MBNPs in different plant species. It appraises the mechanism of nanoparticle-plant interaction in detail and provides understanding of the estimation strategies for the associated pros and cons with this interplay. Critical parameters of NPs include, but are not limited to, particle size and shape, surface chemistry, surface charge, concentration, solubility, and exposure route. On exposure to MBNPs, the molecular, physiological, and biochemical reactions of plants have been assessed. We have filled knowledge gaps and answered research questions regarding the positive and negative effects of metal and metal oxide NPs on seed germination, callus induction, growth and yield of plant, nutritional content, antioxidants, and enzymes. Besides, the phytotoxicity, cytotoxicity, genotoxicity, and detoxification studies of MBNPs in plants have been outlined. Furthermore, the recent developments and future perspectives of the two-way traffic of interplay of MBNPs and plants have been provided in this comprehensive review.

Chaga mushroom: a super-fungus with countless facets and untapped potential.

Inonotus obliquus (Chaga mushroom) is an inexpensive fungus with a broad range of traditional and medicinal applications. These applications include therapy for breast, cervix, and skin cancers, as well as treating diabetes. However, its benefits are virtually untapped due to a limited understanding of its mycochemical composition and bioactivities. In this article, we explore the ethnobotany, mycochemistry, pharmacology, traditional therapeutic, cosmetic, and prospective agricultural uses. The review establishes that several secondary metabolites, such as steroids, terpenoids, and other compounds exist in chaga. Findings on its bioactivity have demonstrated its ability as an antioxidant, anti-inflammatory, antiviral, and antitumor agent. The study also demonstrates that Chaga powder has a long history of traditional use for medicinal purposes, pipe smoking rituals, and mystical future forecasts. The study further reveals that the applications of Chaga powder can be extended to industries such as pharmaceuticals, food, cosmetics, and agriculture. However numerous publications focused on the pharmaceutical benefits of Chaga with few publications on other applications. Overall, chaga is a promising natural resource with a wide range of potential applications and therefore the diverse array of therapeutic compounds makes it an attractive candidate for various applications such as plant biofertilizers and active ingredients in cosmetics and pharmaceutical products. Thus, further exploration of Chaga's potential benefits in agriculture and other industries could lead to exciting new developments and innovations.

Phyto-oxylipin mediated plant immune response to colonization and infection in the soybean-Phytophthora sojae pathosystem.

Introduction: Food security is a major challenge to sustainably supply food to meet the demands of the ever-growing global population. Crop loss due to pathogens is a major concern to overcoming this global food security challenge. Soybean root and stem rot caused by Phytophthora sojae results in approximately 20B $US crop loss annually. Phyto-oxylipins are metabolites biosynthesized in the plants by oxidative transformation of polyunsaturated fatty acids through an array of diverging metabolic pathways and play an important role in plant development and defense against pathogen colonization and infection. Lipid mediated plant immunity is a very attractive target for developing long term resistance in many plants' disease pathosystem. However, little is known about the phyto-oxylipin's role in the successful strategies used by tolerant soybean cultivar to mitigate Phytophthora sojae infection. Methods: We used scanning electron microscopy to observe the alterations in root morphology and a targeted lipidomics approach using high resolution accurate mass tandem mass spectrometry to assess phyto-oxylipin anabolism at 48 h, 72 h and 96 h post infection. Results and discussion: We observed the presence of biogenic crystals and reinforced epidermal walls in the tolerant cultivar suggesting a mechanism for disease tolerance when compared with susceptible cultivar. Similarly, the unequivocally unique biomarkers implicated in oxylipin mediated plant immunity [10(E),12(Z)-13S-hydroxy-9(Z),11(E),15(Z)-octadecatrienoic acid, (Z)-12,13-dihydroxyoctadec-9-enoic acid, (9Z,11E)-13-Oxo-9,11-octadecadienoic acid, 15(Z)-9-oxo-octadecatrienoic acid, 10(E),12(E)-9-hydroperoxyoctadeca-10,12-dienoic acid, 12-oxophytodienoic acid and (12Z,15Z)-9, 10-dihydroxyoctadeca-12,15-dienoic acid] generated from intact oxidized lipid precursors were upregulated in tolerant soybean cultivar while downregulated in infected susceptible cultivar relative to non-inoculated controls at 48 h, 72 h and 96 h post infection by Phytophthora sojae, suggesting that these molecules may be a critical component of the defense strategies used in tolerant cultivar against Phytophthora sojae infection. Interestingly, microbial originated oxylipins, 12S-hydroperoxy-5(Z),8(Z),10(E),14(Z)-eicosatetraenoic acid and (4Z,7Z,10Z,13Z)-15-[3-[(Z)-pent-2-enyl]oxiran-2-yl]pentadeca-4,7,10,13-tetraenoic acid were upregulated only in infected susceptible cultivar but downregulated in infected tolerant cultivar. These microbial originated oxylipins are capable of modulating plant immune response to enhance virulence. This study demonstrated novel evidence for phyto-oxylipin metabolism in soybean cultivars during pathogen colonization and infection using the Phytophthora sojae-soybean pathosystem. This evidence may have potential applications in further elucidation and resolution of the role of phyto-oxylipin anabolism in soybean tolerance to Phytophthora sojae colonization and infection.

Effects of Chilling Stress on Morphological, Physiological, and Biochemical Attributes of Silage Corn Genotypes during Seedling Establishment.

Chilling stress is one of the major abiotic stresses which hinder seedling emergence and growth. Herein, we investigated the effects of chilling/low temperature stress on the morphological, physiological, and biochemical attributes of two silage corn genotypes during the seedling establishment phase. The experiment was conducted in a growth chamber, and silage corn seedlings of Yukon-R and A4177G-RIB were grown at optimum temperature up to V3 stage and then subjected to five temperature regimes (25 °C as control, 20 °C, 15 °C, 10 °C, and 5 °C) for 5 days. After the temperature treatment, the morphological, physiological, and biochemical parameters were recorded. Results indicated that temperatures of 15 °C and lower significantly affected seedling growth, photosynthesis system, reactive oxygen species (ROS) accumulation, and antioxidant enzyme activities. Changes in seedlings' growth parameters were in the order of 25 °C > 20 °C > 15 °C > 10 °C > 5 °C, irrespective of genotypes. The chlorophyll content, photosynthetic rate, and maximal photochemical efficiency of PS-II (Fv/Fm) were drastically decreased under chilling conditions. Moreover, chilling stress induced accumulation of hydrogen peroxide (H2O2)and malonaldehyde (MDA) contents. Increased proline content and enzymatic antioxidants, including superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxide (APX), were found to alleviate oxidative damage under chilling stress. However, the genotype of Yukon-R exhibited better adaption to chilling stress than A4177G3-RIB. Yukon-R showed significantly higher proline content and enzymatic antioxidant activities than A4177G3-RIB under severe chilling conditions (temperature ≤ 10 °C). Similarly, Yukon-R expressed low temperature-induced ROS accumulation. Furthermore, the interaction effects were found between temperature treatment and genotype on the ROS accumulation, proline content and antioxidant enzyme activities. In summary, the present study indicated that Yukon-R has shown better adaptation and resilience against chilling temperature stress, and therefore could be considered a potential candidate genotype to be grown in the boreal climate.

Potential of developing podzolic soil-based potting media from wood ash, paper sludge and biochar.

BACKGROUND AND OBJECTIVES: Organic waste management in environmentally sustainable way is important not only to reduce the negative impacts on ecosystems but also valorizing the waste resources. Herein we evaluated the potential of wood ash (WA) and paper sludge (PS) wastes from a pulp and paper mill as potting media and their effects on the physicochemical properties of podzolic soil. METHODS: WA, PS and biochar (BC) was mixed in different combinations with a sandy loam podzolic soil. Potting media treatments included: T1-soil (negative control); T2-PromixTM (positive control); T3-50%soil+50%WA; T4-75%soil+25%WA; T5-50%soil+50%PS; T6-75%soil+25%PS; T7-75%soil+25%BC; T8-25%soil+50%WA+25%BC; T9-50%soil+25%WA+25%BC; T10-25%soil+50%PS+25%BC; T11-50%soil+25%PS+25%BC, T12- 25%soil+25%WA+25%PS+25%BC and replicated three times. RESULTS: Potting media treatments expressed significant (p < 0.00) effects on pH, bulk density, total porosity, field capacity, plant available water (PAW) and water retention curves. Potting media amended with WA showed high pH range (8-12) while PS amendments exhibited pH in range where most plant nutrients are available (6.5-7.5). Results depicted significantly lower bulk density, and increased total porosity and water holding capacity of potting media amended with WA and PS. BC addition further enhanced the water retention properties compared to combinations without BC. T6, T10 and T11 produced higher amounts of PAW with desired pH compared to T1 and T2. CONCLUSION: WA, PS and BC showed high potential for developing podzolic soil-based potting media, but their effects on plant growth and elemental uptake need to be investigated.

Data set showing the development of a hyperspectral imaging technique using LA-ICP-MS to determine the spatial distribution of nutrients in soil cores.

This data in brief article represents the data set associated with a research article published in Geoderma [1]. The data set represents figures showing the spatial distribution of selected macro and micronutrients, and their quantification in different crop or nutrient management systems practiced in the boreal ecosystem. Spatial distribution of nutrients was measured by laser ablation inductively coupled plasma mass spectrometry (LA‒ICP‒MS), using the new techniques we developed to visualize nutrient distribution in intact soil cores representative of the root rhizosphere. This data article supports the findings published in the main article [1]. This work also demonstrates that LA-ICP-MS is a valuable technique to image the spatial distribution of macro and micronutrients in intact soil cores as affected by different crop management practices.

Corn-Soybean Intercropping Improved the Nutritional Quality of Forage Cultivated on Podzols in Boreal Climate.

Intercropping systems could be a potential source of nutrient-rich forage production in cool climates on podzolic soils common in boreal ecosystems. In this study, we evaluated the effects of corn-soybean intercropping (IC) on the nutritional quality of forage. Two silage corn varieties were cultivated as monocropping (MC) or were intercropped with three forage soybean varieties using a randomized complete block design. IC significantly increased the crude protein (22%) and decreased the acid detergent (14%) and neutral detergent (6%) fibers. Forage net energy, total digestible nutrients, ash, dry matter intake, digestible dry matter and relative feed value were also significantly increased (p ≤ 0.05) in the IC treatments compared to corn MC. The macro and micro nutrients were higher in IC than corn MC. Intercropping increased the omega 3 fatty acid (FA) contents (67%) compared to corn MC. IC also increased the active microbial community in the plant root zone, which may contribute to the improvement in forage nutritional quality because the active soil microbial community composition showed significant correlations with soluble sugars, soluble proteins and potassium contents of the forage. These results demonstrate that corn-soybean IC could be a suitable cropping system to increase the nutritional quality of forage cultivated on podzols in boreal climates. The resultant forage has the potential to be a source of high-value animal feed for livestock production in cool climate regions of the world.

Carbon Nanotubes Improved the Germination and Vigor of Plant Species from Peatland Ecosystem Via Remodeling the Membrane Lipidome.

Application of the nanopriming technique to alleviate seed dormancy has shown promising results in various agricultural crop species. However, there is a dearth of knowledge regarding its potential use in native peatland boreal forest species to alleviate seed dormancy and improve their propagation or vigor for forest reclamation activities. Herein, we demonstrate the use of nanopriming with carbon nanotubes (CNT) to alleviate seed dormancy, improved seed germination, and seedling vigor in two boreal peatland species. Bog birch (Betula pumila L.) and Labrador tea (Rhododendron groenlandicum L.) seeds with embryo or seed coat dormancy were nanoprimed with either 20 or 40 µg/mL CNT, cold stratified at 2-4 °C for 15 days, and allowed to germinate at room temperature. The emerged seedlings' lipidome was assessed to decipher the role of lipid metabolism in alleviating seed dormancy. We observed significant (p < 0.05) improvement in seedling germination and seedling vigor in seeds primed with multiwalled carbon nanotubes functionalized with carboxylic acids. Phosphatidylcholine (PC 18:1/18:3), phosphatidylglycerol (PG 16:1/18:3), and lysophosphatidylcholine (LPC 18:3) molecular species (C18:3 enriched) were observed to be highly correlated with the increased seed germination percentages and the enhanced seedling vigor. Mechanistically, it appears that carbon nanoprimed seeds following stratification are effective in mediating seed dormancy by remodeling the seed membrane lipids (C18:3 enriched PC, PG, and LPC) in both peatland boreal forest species. The study results demonstrate that nanopriming may provide a solution to resolve seed dormancy issues by enhancing seed germination, propagation, and seedling vigor in non-resource boreal forest species ideally suited for forest reclamation following anthropogenic disturbances.

Root membrane lipids as potential biomarkers to discriminate silage-corn genotypes cultivated on podzolic soils in boreal climate.

Root membrane lipids are important biomolecules determining plant's ability to adapt to different growing environmental or climatic conditions. Herein, we demonstrate the potential use of root membrane lipids as biomarkers to discriminate silage-corn genotypes based on herbicide and insect/pest resistance genetic traits when cultivated on podzolic soils under short growing and moderately warm summer season in boreal climate. Lipids in root membranes of field grown silage-corn genotypes were previously quantified at crop maturity by ultra-high-performance liquid chromatography-hydrophilic interaction chromatography-heated electrospray ionization mass spectrometry. The lipid identified and quantified in silage-corn roots were phospholipids, glycolipids and sphingolipids. Following hierarchical cluster analysis, three groups of membrane lipids were observed to be very effective in segregating the five silage-corn genotypes. The first group consisted of hexosylceramide (HexCer), phosphatidylcholine (PC) and phosphatidylinositol (PI). The second group consisted of lysophosphatidic acid (LPA16:0) and lysophosphatidylcholine (LPC16:0), while the third group consisted of 37 molecular species from observed lipids (phospholipids, glycolipids, sphingolipids). Partial least squares-discriminant analysis (PLS-DA) based on 37 membrane lipid species, as well as principal component analysis using the variables important in projection derived from the PLS-DA segregated the five silage-corn genotypes into three groups according to their pesticide/herbicide resistant traits. This study is second to none using root lipidomics in discriminating different silage-corn genotypes based on their herbicide and insect/pest resistance genetic traits for cultivation in boreal climates. The segregated genotypes possess three different genetic traits for herbicide and insect/pest resistance including VT Double Pro (VT2P), VT Triple Pro Roundup Ready (VT3P/RR) and Roundup Ready-2 corn (RR2). These findings demonstrate that root membrane lipids could serve as appropriate chemical biosignatures to identify silage-corn genotypes based on herbicide and insect/pest resistance genetic traits suitable for cultivation in boreal climates.

Canola produced under boreal climatic conditions in Newfoundland and Labrador have a unique lipid composition and expeller press extraction retained the composition for commercial use.

The average fatty acid (FA) composition of canola oil is made up of 62% oleic acid (C18:1n9), 19% linoleic acid (C18:2n6), 9% linolenic acid (C18:3n3) and 7% saturated FA (SFA). We investigated whether boreal climate (7.5-17.2 °C) favorably altered the FA composition of canola. Results indicate that canola cultivated in boreal climatic conditions had approximately twice the levels of omega-3 FA (17-20%) compared to canola from other growing areas (9%). The presence of monoacetyldiacylglycerol (MAcDG), a unique class of triglyceride, is reported for the first time in canola cultivated in a boreal climate, and has the potential to reduce the risk of obesity and other health related diseases. We further demonstrated that a non-solvent based extraction method retained the novel lipid composition without reducing the quality of oil being produced. Our results contribute significantly to the understanding of lipid accumulation in the world's second most important oil crop when cultivated in a boreal or northern climate.

Biochar amendment mitigates greenhouse gases emission and global warming potential in dairy manure based silage corn in boreal climate.

About 11% of the global anthropogenic greenhouse gases (GHGs) emissions result from agricultural practices. Dairy manure (DM) application to soil is regarded as a best management practice due to C sequestration and improvement of soil physiochemical properties. However, GHGs emissions from the soil following the DM application could offset its advantages. Biochar (BC) is known to affect N transformation and GHGs emissions from soil. There had been considerably less focus on the BC amendment and its effects on GHGs emissions following DM application under field conditions. The objectives of this study were; i) to determine the temporal patterns and cumulative GHGs fluxes following DM and inorganic nitrogen (IN) application and, ii) to investigate BC amendment impact on DMY, GWP, direct N2O emission factor (EFd) and the response of CH4 emissions (RC) in DM based silage corn. To achieve these objectives a two-year field experiment was conducted with these treatments: 1) DM with high N conc. (DM1: 0.37% N); 2) DM with low N conc. (DM2: 0.13% N); 3) IN; 4) DM1+BC; 5) DM2+BC; 6) IN + BC; and 7) Control (N0); and were laid out in randomized complete block design with four replications. BC amendment to DM1, DM2 and IN significantly reduced cumulative CO2 emission by 16, 25.5 and 26.5%, CH4 emission by 184, 200 and 293% and N2O emission by 95, 86 and 93% respectively. It also reduced area-scaled and yield-scaled GWP, EFd, RC and enhanced DMY. Thus, BC application showed great potential to offset the negative effects of DM application i.e GHGs emissions from the silage corn cropping system. Further research is needed to evaluate soil organic carbon and nitrogen dynamics (substrates for GHG emissions) after DM and BC application on various soil types and cropping systems under field conditions.

Comparison of Multi-Frequency and Multi-Coil Electromagnetic Induction (EMI) for Mapping Properties in Shallow Podsolic Soils.

Electromagnetic induction (EMI) technique is an established method to measure the apparent electrical conductivity (ECa) of soil as a proxy for its physicochemical properties. Multi-frequency (MF) and multi-coil (MC) are the two types of commercially available EMI sensors. Although the working principles are similar, their theoretical and effective depth of investigation and their resolution capacity can vary. Given the recent emphasis on non-invasive mapping of soil properties, the selection of the most appropriate instrument is critical to support robust relationships between ECa and the targeted properties. In this study, we compared the performance of MC and MF sensors by their ability to define relationships between ECa (i.e., MF-ECa and MC-ECa) and shallow soil properties. Field experiments were conducted under wet and dry conditions on a silage-corn field in western Newfoundland, Canada. Relationships between temporally stable properties, such as texture and bulk density, and temporally variable properties, such as soil water content (SWC), cation exchange capacity (CEC) and pore water electrical conductivity (ECw) were investigated. Results revealed significant (p < 0.05) positive correlations of ECa to silt content, SWC and CEC for both sensors under dry conditions, higher correlated for MC-ECa. Under wet conditions, correlation of MF-ECa to temporally variable properties decreased, particularly to SWC, while the correlations to sand and silt increased. We concluded that the MF sensor is more sensitive to changes in SWC which influenced its ability to map temporally variable properties. The performance of the MC sensor was less affected by variable weather conditions, providing overall stronger correlations to both, temporally stable or variable soil properties for the tested Podzol and hence the more suitable sensor toward various precision agricultural practices.

Carbon Nanoparticles Functionalized with Carboxylic Acid Improved the Germination and Seedling Vigor in Upland Boreal Forest Species.

Nanopriming has been shown to significantly improve seed germination and seedling vigor in several agricultural crops. However, this approach has not been applied to native upland boreal forest species with complex seed dormancy to improve propagation. Herein, we demonstrate the effectiveness of carbon nanoparticles functionalized with carboxylic acids in resolving seed dormancy and improved the propagation of two native upland boreal forest species. Seed priming with carbon nanoparticles functionalized with carboxylic acids followed by stratification were observed to be the most effective in improving germination to 90% in green alder (Alnus viridis L.) compared to 60% in the control. Conversely, a combination of carbon nanoparticles (CNPs), especially multiwall carbon nanoparticles functionalized with carboxylic acid (MWCNT-COOH), cold stratification, mechanical scarification and hormonal priming (gibberellic acid) was effective for buffaloberry seeds (Shepherdia canadensis L.). Both concentrations (20 µg and 40 µg) of MWCNT-COOH had a higher percent germination (90%) compared to all other treatments. Furthermore, we observed the improvement in germination, seedling vigor and resolution of both embryo and seed coat dormancy in upland boreal forest species appears to be associated with the remodeling of C18:3 enriched fatty acids in the following seed membrane lipid molecular species: PC18:1/18:3, PG16:1/18:3, PE18:3/18:2, and digalactosyldiacylglycerol (DGDG18:3/18:3). These findings suggest that nanopriming may be a useful approach to resolve seed dormancy issues and improve the seed germination in non-resource upland boreal forest species ideally suited for forest reclamation following resource mining.

The potential of corn-soybean intercropping to improve the soil health status and biomass production in cool climate boreal ecosystems.

Intercropping (IC) is a promising approach used to improve soil health and sustainable crop production. However, it is unknown whether IC improve the soil health status and biomass productivity of crops cultivated in podzols under cool climate in boreal ecosystems. Two silage corn and three forage soybean genotypes were cultivated either as inter or monocrop (MC) treatments in a randomized complete block design. IC resulted in 28% increase in total forage production (FP). A reduction in rhizosphere soil pH (RS-pH) was observed in the IC treatments. Conversely, the rhizosphere soil acid phosphatase (RS-APase) activity was significantly higher (26-46%) in the IC treatments and occurred concomitant with a significant increase in available phosphorus (RS-Pavailable) (26-74%) in the rhizosphere. Furthermore, IC enhanced the active microbial composition and strong positive correlations were observed between RS-Pavailable, RS-APase, microbial biomass and FP; while RS-pH was negatively correlated with FP, RS-APase and RS-Pavailable. These findings suggested silage corn intercropped with forage soybean could be a viable approach to enhance FP through improved active microbial community structure, RS-APase activity and RS-Pavailable when cultivated on podzols in cool climate boreal ecosystem.

The soybean root membrane lipids and forage quality data in response to field cultivation on agricultural podzols in boreal climates.

The objective of this data in brief article is to represent the associated data set regarding our published paper in Plant Science Nadeem et al., 2019. Data set represent soil acid phosphatase activity, association of individual molecular species of four major lipid classes with soybean forage quality indices when cultivated in boreal podzolic soils under cool climatic conditions. Phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) and acylated glucosyl betasitosterol ester (AGIcSiE) molecular species grouped the soybean forage quality indices in different quadrants on principal component analyses. Furthermore, the total lipid profile and correlation of major lipid species with forage quality indices are included in this data in brief article. This data set support the main findings described in Nadeem et al., 2019.

Adaptation strategies of forage soybeans cultivated on acidic soils under cool climate to produce high quality forage.

Boreal soils tend to be podzols characterized by acidic pH, which can further limit forage crop growth and production. It is unclear, how forage soybeans adopt to produce forage with high nutritional quality when cultivated on podzols in boreal climate. To answer this question, we cultivated forage soybeans on agricultural podzols at 3 farm sites with varied soil pH (6.8, 6.0 or 5.1), and assessed the root membrane lipidome remodeling response to such climatic conditions. Contrary to our expectations, significantly lower biomass was observed at pH 6.8 compared to 6.0 and 5.1. However, surprisingly the plants produced similar forage quality at 6.8 and 5.1 pH. Three major lipid classes including phospholipids, glycolipids and phytosterols were observed in roots irrespective of soil pH. Phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA), and acylated glucosyl betasitosterol ester (AGlcSiE) accounted for 95% of the root lipidome, and expressed significant changes in response to cultivation across the three soil pH levels. These lipids were also observed to have strong correlations with forage production, and forage quality. Therefore, soybean genotypes with higher abilities to remodel PC, PE, PA, and AGlcSiE could be better suited for producing higher quality forage in acid podzolic soils characteristics of boreal ecosystems.

Potential role of root membrane phosphatidic acid in superior agronomic performance of silage-corn cultivated in cool climate cropping systems.

The literature is replete with information describing the composition of the root lipidome in several plant species grown under various environmental conditions. However, it is unknown to what extent the root membrane lipidome vary between silage-corn genotypes, and how such variation could influence agronomic performances during field cultivation in cool climate. To address this issue, the root membrane lipidome and agronomic performance were assessed for five silage-corn genotypes (Fusion-RR, Yukon-R, A4177G3-RIB, DKC23-17RIB, DKC26-28RIB) cultivated under cool climatic conditions. Leaf area, plant height and biomass production were used as agronomic performance indicators. Varieties DKC26-28RIB and Yukon-R expressed significantly higher leaf area, plant height and biomass production compared to the other genotypes. A strong positive Spearman rank-order correlation (P = 0.001) was observed between biomass production and root phosphatidic acid (PA). The high correlation observed between PA and agronomic performance indicates PA could potentially be used as biomarker to assist in the selection of silage-corn genotypes with superior agronomic performance ideally suited for field cultivations in cool climatic conditions.

The use of natural media amendments to produce kale enhanced with functional lipids in controlled environment production system.

Diets high in vegetable consumption is highly correlated with reduced risk of developing common lifestyle related diseases. We investigated the effects of three natural growth media amendments [potassium humate, dry vermicast, volcanic minerals or Promix alone (Control)] in enhancing the accumulation of functional lipids in greenhouse grown kale. Functional lipids (n9, n6, n3 fatty acids, diglycerides, galactolipids and phytosterols) were assessed using either gas chromatography/mass spectrometry (GC/MS) or ultra-high performance liquid chromatography-high resolution tandem mass spectrometry (UHPLC-HRMS/MS). The results showed volcanic minerals and dry vermicast were the most successful in enhancing the accumulation of functional lipids in kale. For example, dry vermicast enhanced the accumulation of total C18:1n9 and C16:3n3 fatty acids, while total C18:2n6 fatty acid accumulation was enhanced by volcanic minerals. In conclusion, natural growing medium amendments are remarkably effective in modulating the accumulation of functional lipids in kale grown under controlled-environment conditions. This could be a useful strategy for functional foods production in control environment production systems. Increase access to kale with enhanced functional lipids could aid in increase consumption of these health promotive compounds in the diet with potential implications in population health.

Northward shift of the agricultural climate zone under 21st-century global climate change.

As agricultural regions are threatened by climate change, warming of high latitude regions and increasing food demands may lead to northward expansion of global agriculture. While socio-economic demands and edaphic conditions may govern the expansion, climate is a key limiting factor. Extant literature on future crop projections considers established agricultural regions and is mainly temperature based. We employed growing degree days (GDD), as the physiological link between temperature and crop growth, to assess the global northward shift of agricultural climate zones under 21st-century climate change. Using ClimGen scenarios for seven global climate models (GCMs), based on greenhouse gas (GHG) emissions and transient GHGs, we delineated the future extent of GDD areas, feasible for small cereals, and assessed the projected changes in rainfall and potential evapotranspiration. By 2099, roughly 76% (55% to 89%) of the boreal region might reach crop feasible GDD conditions, compared to the current 32%. The leading edge of the feasible GDD will shift northwards up to 1200 km by 2099 while the altitudinal shift remains marginal. However, most of the newly gained areas are associated with highly seasonal and monthly variations in climatic water balances, a critical component of any future land-use and management decisions.