Importance of the Ultrasound Probe Angle on the Rotation Fill-in Signature in Ultrasound Axial-Shear Strain Imaging.

The rotation fill-in is a signature of tumor benignity in rotation elastograms and has been used for breast tumor classification. It is a consequence of the bonding condition at the tumor-tissue interface. In vivo studies have revealed the presence of fluctuations when inclined uniaxial external compression is applied. However, the physical meaning of these fluctuations is not yet fully understood. In this article we present an experimental and numerical study of the rotation fill-in signature as a function of the probe's tilt angle. This angle introduces asymmetries in the stress field, modifying the bonding condition. We numerically consider this asymmetry by using a model of friction with a simple angular dependence, which allows us to capture the experimental trends. We argue that the formulation of a tumor model with a bonding condition dependence may have potential implications in correct tumor classification.

Optimization of a Pixel-to-Pixel Curve-Fitting Method for Poroelastography Imaging.

Ultrasound poroelastography is an imaging modality used to characterize the temporal behavior of soft tissue that can be modeled as a solid permeated by interconnected pores filled with liquid (poroelastic medium). It could be useful in the stage classification of lymphedema. Generally, time-constant models are applied to strain images, and precision of the fitting process, computational cost and versatility in response to changes in tissues properties are crucial aspects of clinical applications. In the work described here, we performed creep experiments on poroelastic phantoms and used rheologic models to visualize the changes in viscoelastic response associated with fluid mobility. We used the Levenberg-Marquardt algorithm as a fitting tool and performed parametric studies to improve its performance. On the basis of these studies, we proposed an optimization schema for the pixel-to-pixel curve-fitting process. We determined that the bimodal Kelvin-Voigt model describes efficiently the temporal evolution of the strain images in heterogeneous phantoms.

Creep of sound paths in consolidated granular material detected through coda wave interferometry.

The time evolution of the contact force structure of a consolidated granular material subjected to a constant stress is monitored using the coda wave interferometry method. In addition, the nature of the aging and rejuvenation processes are investigated. These processes are interpreted in terms of affine and nonaffine structural path deformations. During the later stages of creep, the rearrangements of subgrains are so small that they only produce affine deformations in the contact paths, without any significant changes in the structural configuration. As a result, the strain path distribution follows the macroscopic strain. Conversely, in the presence of ultrasonic perturbations, the nonaffine grain buckling mechanism dominates, producing relatively drastic changes in the structural configuration accompanied by path deformations of the order of the grain size. This plastic mechanism induces material rejuvenation that is observed macroscopically as an ultrasonically accelerated creep.

Ultrasound induces aging in granular materials.

Aging and rejuvenation have been identified as the general mechanisms that rule the time evolution of granular materials subjected to some external confinement pressure. In creep experiments performed in a triaxial configuration, we obtained evidence that relatively high intensity ultrasound waves propagating through the material induce both weakening and significant plasticity. In the framework of glassy materials, it is shown that the effect of ultrasound can be simply accounted for by a general variable, the fluidity, whose dynamics are described by an effective aging parameter that strongly decreases with sound amplitude and vanishes at the yield stress limit. The response from step perturbations in ultrasound intensity provided a method to assess the effective-viscosity jumps which are direct evidence of acoustic fluidization.

On the advantages of imaging the axial-shear strain component of the total shear strain in breast tumors.

Axial-shear strain elastography was described recently as a method to visualize the state of bonding at an inclusion boundary. Although total shear strain elastography was initially proposed for this purpose, it did not evolve beyond the initial reported finite element model (FEM) and simulation studies. One of the major reasons for this was the practical limitation in estimating the tissue motion perpendicular (lateral) to the ultrasound (US) beam as accurately as the motion along the US beam (axial). Nevertheless, there has been a sustained effort in developing methods to improve the lateral motion tracking accuracy and thereby obtain better quality total shear strain elastogram (TSSE). We hypothesize that in some cases, even if good quality TSSE becomes possible, it may still be advantageous to utilize only the axial-shear strain (one of the components of the total shear strain) elastogram (ASSE). Specifically, we show through FEM and corroborating tissue-mimicking gelatin phantom experiments that the unique "fill-in" discriminant feature that was introduced recently for asymmetric breast lesion classification is depicted only in the ASSE and not in the TSSE. Note that the presence or conspicuous absence of this feature in ASSE was shown to characterize asymmetric inclusions' boundaries as either loosely-bonded or firmly-bonded to the surrounding, respectively. This might be an important observation because the literature suggests that benign breast lesions tend to be loosely-bonded, while malignant tumors are usually firmly-bonded. The results from the current study demonstrate that the use of shear strain lesion "fill-in" as a discriminant feature in the differentiation between asymmetric malignant and benign breast lesions is only possible when using the ASSEs and not the TSSEs.

Two-dimensional simulation of linear wave propagation in a suspension of polymeric microcapsules used as ultrasound contrast agents.

A generation of tissue-specific stable ultrasound contrast agent (UCA) composed of a polymeric capsule with a perfluorocarbone liquid core has become available. Despite promising uses in clinical practice, the acoustical behavior of such UCA suspensions remains unclear. A simulation code (2-D finite-difference time domain, FDTD) already validated for homogeneous particles [Galaz Haiat, Berti, Taulier, Amman and Urbach, (2010). J. Acoust. Soc. Am. 127, 148-154] is used to model the ultrasound propagation in such UCA suspensions at 50 MHz to investigate the sensitivity of the ultrasonic parameters to physical parameters of UCA. The FDTD simulation code is validated by comparison with results obtained using a shell scatterer model. The attenuation coefficient (respectively, the sound velocity) increases (respectively, decreases) from 4.1 to 58.4 dB/cm (respectively, 1495 to 1428 m/s) when the concentration varies between 1.37 and 79.4 mg/ml, while the backscattered intensity increases non-linearly, showing that a concentration of around 30 mg/ml is sufficient to obtain optimal backscattering intensity. The acoustical parameters vary significantly as a function of the membrane thickness, longitudinal and transverse velocity, indicating that mode conversions in the membrane play an important role in the ultrasonic propagation. The results may be used to help manufacturers to conceive optimal liquid-filled UCA suspensions.

Visualization of HIFU-induced lesion boundaries by axial-shear strain elastography: a feasibility study.

In this paper, we report on a study that investigated the feasibility of reliably visualizing high-intensity focused ultrasound (HIFU) lesion boundaries using axial-shear strain elastograms (ASSE). The HIFU-induced lesion cases used in the present work were selected from data acquired in a previous study. The samples consisted of excised canine livers with thermal lesions produced by a magnetic resonance-compatible HIFU system (GE Medical System, Milwaukee, WI, USA) and were cast in a gelatin block for the elastographic experiment. Both single and multiple HIFU-lesion samples were investigated. For each of the single-lesion samples, the lesion boundaries were determined independently from the axial strain elastogram (ASE) and ASSE at various iso-intensity contour thresholds (from -2 dB to -6 dB), and the area of the enclosed lesion was computed. For samples with multiple lesions, the corresponding ASSE was analyzed for identifying any unique axial-shear strain zones of interest. We further performed finite element modeling (FEM) of simple two-inclusion cases to verify whether the in vitro ASSE obtained were reasonable. The results show that the estimation of the lesion area using ASSE is less sensitive to iso-intensity threshold selection, making this method more robust compared with the ASE-based method. For multiple lesion cases, it was shown that ASSE enables high-contrast visualization of a "thin" untreated region in between multiple fully-treated HIFU-lesions. This contrast visualization was also noticed in the FEM predictions. In summary, the results demonstrate that it is feasible to reliably visualize HIFU lesion boundaries using ASSE.

Importance of axial compression verification to correct interpretation of axial-shear strain elastograms in breast lesions.

We have recently shown that the appearance of Axial-Shear Strain Elastograms (ASSEs) for the case of loosely-bonded, elliptical inclusions (like fibroadenomas in the breast) is unique and therefore has the potential to distinguish benign fibroadenomas from malignant tumors in the breast. The ASSEs were obtained using quasi-static axial compressions, in a like manner as in normal axial-strain elastography. However, strict axial compression is achieved most often only by computer-controlled acquisitions and not by more practical freehand acquisitions. In a freehand acquisition, the frame sequence may contain several frames that do not experience strict axial compression but may also experience rotation or shear deformations. In this paper, we demonstrate the importance of accounting for the type of deformation applied to a target tissue for the correct interpretation of the resulting ASSEs. Using freehand acquired in vivo examples, we show that such a frame experiencing rotation or shear deformations results in ASSEs that may potentially be misinterpreted. This may be far more detrimental compared to the corresponding axial elastogram frames that may only suffer from inferior image quality in terms of contrast-to-noise ratio (CNR). Further, we show that we may be able to eliminate these frames from a sequence of freehand acquired in vivo breast lesion data by implementing a special filtering scheme, thus significantly improving the reliability of the remaining ASSE frames. This work further suggests that under freehand conditions, frames have to be checked for the presence of undesirable deformations.

Axial-shear strain distributions in an elliptical inclusion model: experimental validation and in vivo examples with implications to breast tumor classification.

Recently, we reported on the axial-shear strain fill-in of the interior of loosely bonded stiff elliptical inclusions in a soft background at non-normal orientations, and the lack of fill-in in firmly bonded inclusions at any orientation. In this paper, we report on the experimental validation of the simulation studies using tissue-mimicking gelatin-based phantoms. We also show a few confirmatory examples of the existence of these phenomena in benign vs. malignant breast lesions in vivo. Phantom experiments showed that axial-shear strain zones caused by firmly bonded elliptical inclusions occurred only outside of the inclusion, as predicted by the simulation. By contrast, the axial-shear strain zones filled in the interior of loosely bonded elliptical inclusions at non-normal orientations. The axial-shear strain elastograms obtained from the in vivo cases appeared to be in general agreement with our experimental results. The results reported in this paper may have important clinical implications. Specifically, axial-shear strain fill-in inside an inclusion may be a unique signature of stiff, loosely bonded, ellipsoidal or elongated inclusions at non-normal orientations. Thus, it may be useful as a marker of benignity of benign breast lesions (e.g., fibroadenomas) that are generally stiff, elongated and loosely bonded to the host tissues.

Experimental validation of a time domain simulation of high frequency ultrasonic propagation in a suspension of rigid particles.

Ultrasonic propagation in suspensions of particles is a difficult problem due to the random spatial distribution of the particles. Two-dimensional finite-difference time domain simulations of ultrasonic propagation in suspensions of polystyrene 5.3 mum diameter microdisks are performed at about 50 MHz. The numerical results are compared with the Faran model, considering an isolated microdisk, leading to a maximum difference of 15% between the scattering cross-section values obtained analytically and numerically. Experiments are performed with suspensions in through transmission and backscattering modes. The attenuation coefficient at 50 MHz (alpha), the ultrasonic velocity (V), and the relative backscattered intensity (I(B)) are measured for concentrations from 2 to 25 mg/ml, obtained by modifying the number of particles. Each experimental ultrasonic parameter is compared to numerical results obtained by averaging the results derived from 15 spatial distributions of microdisks. alpha increases with the concentration from 1 to 17 dB/cm. I(B) increases with concentration from 2 to 16 dB. The variation of V versus concentration is compared with the numerical results, as well as with an effective medium model. A good agreement is found between experimental and numerical results (the larger discrepancy is found for alpha with a difference lower than 2.1 dB/cm).

Ultrasonic sound velocity measurement in samples of soft materials through under-resonance excitation.

Ultrasound (US) velocity determination is a valuable characterization technique, providing important information on elastic properties of materials. Sound velocity can be obtained accurately in the pulsed method if the thickness of the specimen is precisely known. This is clearly not easily achievable for soft materials, such as biologic soft tissues or tissue-mimicking phantoms. From this consideration, previous works have established that sound velocity can be determined in through-transmission configuration without thickness measurement through the time-of-flight determination of specimen-reflected echoes in plane parallel-surfaced specimens. It is shown here that the amplitude and shape of these specimen echoes can be significantly improved by working in the tone-burst mode at an excitation frequency below the transducer resonance. This is particularly valuable for materials presenting a low acoustic contrast with the surrounding medium, usually water, such as tissue-mimicking materials and water-based phantoms, making the specimen echo time-of-flights and, consequently, the sound velocity determination, more reliable.

Sound velocity determination in gel-based emulsions.

Sound velocity is a main parameter in non destructive characterization, closely related to the elastic properties and to the microstructure of heterogeneous materials. The accurate determination of the sound velocity using pulse-echo technique relies on the ability to reduce pulse distortion and to measure specimen dimensions with a high precision. In the field of bio-mimetic materials and biological tissues, the nature of the specimen makes this last requirement highly difficult or inappropriate. The present work, using a through-transmission configuration, allows, in a stress free environment, to access the sound velocity in soft, low acoustic contrast materials without requiring the specimen dimensions. The specimen sound velocity is obtained from the echo time-of-flights through a Z-scan process providing the absolute medium sound velocity as reference. The technique uses an excitation burst at a frequency below the transducer resonance to ensure a significantly reduction in pulse distortions and improve signal-to-noise ratio. The accurate determination of the echo time-of-flight relies on a highly efficient cross-correlation/Hilbert transform signal processing. The method has been applied to gel-based emulsions of different microstructures considered as biomimetic phantoms, as well as to their constituents: pure gelatin and vegetable oil.