ABSTRACT
The investigation was performed on 75 of Golden Retriever puppies. Faecal samples were collected on the 42 day of the puppies life (con-trol). Probiotic preparation was administered on 43 day of the puppies life and 10 days after the application of the probiotic, faecal samples were collected again (on 53 day of puppies life). All isolates of Campylobacter coli isolated prior to the administration of the probiotic were found to contain the cadF gene responsible for adhesion, as well as, the flaA gene influencing motility of the examined bacteria. Significant differences (P < 0.05) were recorded only in the case of enrofloxacin.
ABSTRACT
The investigation was performed on 75 of Golden Retriever puppies. Faecal samples were collected on the 42 day of the puppies life (control). Probiotic preparation was administered on 43 day of the puppies life and 10 days after the application of the probiotic, faecal samples were collected again (on 53 day of puppies life). All isolates of Campylobacter coli isolated prior to the administration of the probiotic were found to contain the cadF gene responsible for adhesion, as well as, the flaA gene influencing motility of the examined bacteria. Significant differences (P < 0.05) were recorded only in the case of enrofloxacin.
Subject(s)
Bacterial Proteins/metabolism , Campylobacter coli/metabolism , Dog Diseases/microbiology , Animals , Campylobacter coli/drug effects , Campylobacter coli/genetics , Campylobacter coli/pathogenicity , Dogs , Drug Resistance, Bacterial , Feces/microbiology , Gene Expression Regulation, Bacterial/physiology , VirulenceABSTRACT
This study was conducted to determine the prevalence of Campylobacter spp. isolated from dogs' faecal samples. From June 2012 to June 2013, a total of 210 faecal samples from pet dogs living in different kennels (n = 210) were collected by the owners in Greater Poland Voivodeship, Poznan District, Poland. The study revealed that 105 out of 210 faecal samples (50%) contained Campylobacter. The highest prevalence of Campylobacter spp. occurred in spring (81%), followed by winter (64%). The cadF gene was found in 100% of the isolates tested. The occurrence of the other genes was variable. The isolates from young dogs were characterised by higher occurrence of virulence genes.
Subject(s)
Bacterial Proteins/metabolism , Campylobacter Infections/veterinary , Campylobacter/isolation & purification , Dog Diseases/microbiology , Gene Expression Regulation, Bacterial/physiology , Animals , Bacterial Proteins/genetics , Campylobacter/classification , Campylobacter/metabolism , Campylobacter/pathogenicity , Campylobacter Infections/microbiology , Dogs , Feces/microbiology , Poland , Prevalence , Seasons , VirulenceABSTRACT
All mineral elements have their place in nature as macroelements, microelements and submicroelements. They should be delivered to the organism in the proper composition and amount and in the form easy assimilated by the organism. Creating the food composition we should take into account not only energy metabolism and digestible protein content, but also the content of minerals components, among them of selenium. Selenium is assimilated by the organism in the form of selenomethionine and selenocysteine as well as certain inorganic compounds. This element in the excess is a poison, but on the other hand it is essential food component, because of its roles in the metabolism, as well as anticancer factor and antioxidant.
Subject(s)
Deficiency Diseases/diet therapy , Deficiency Diseases/veterinary , Selenium/deficiency , Selenium/metabolism , Animal Feed/analysis , Animals , Antineoplastic Agents/therapeutic use , Antioxidants/administration & dosage , Cattle , Cattle Diseases/diet therapy , Cattle Diseases/metabolism , Dietary Supplements , Neoplasms/drug therapy , Selenium/analysis , Selenium Compounds/therapeutic useABSTRACT
Numerous studies have shown that consumption of soybean products decrease the risk of cancers in humans. Experiments at the molecular level have demonstrated that in most cases proteins and peptides are responsible for the anticancer properties of soybeen. Special attention should be paid to lunasin - a peptide described for the first time 16 years ago. Due to its structure it causes i.a., inhibition of cancer cell proliferation. A novel procedure for the isolation and purification of low-molecular-mass 2S soybean albumin protein is described in the present paper. A fraction of four peptides one of them corresponding to molecular mass and isoelectric point characteristic for lunasin. The obtained peptides decreased on the rate of HeLa cell proliferation.
Subject(s)
Albumins/isolation & purification , Antineoplastic Agents/isolation & purification , Soybean Proteins/isolation & purification , Albumins/pharmacology , Antineoplastic Agents/pharmacology , Cell Proliferation/drug effects , Electrophoresis, Polyacrylamide Gel , HeLa Cells , Humans , Soybean Proteins/chemistryABSTRACT
The aim of this study was to compare Toxoplasma gondii infection in three canid species: red fox Vulpes vulpes, arctic fox Vulpes lagopus and raccoon dog Nyctereutesprocyonoides kept at the same farm. Anal swabs were taken from 24 adult and 10 juvenile red foxes, 12 adult arctic foxes, three adult and seven juvenile raccoon dogs. Additionally, muscle samples were taken from 10 juvenile red foxes. PCR was used to detect T. gondii DNA. T. gondii infection was not detected in any of the arctic foxes; 60% ofraccoon dogs were infected; the prevalence of the parasite in material from red fox swabs was intermediate between the prevalence observed in arctic foxes and raccoon dogs. It is possible that susceptibility and immune response to the parasite differ between the three investigated canid species. T. gondii DNA was detected in muscle tissue of five young foxes. The results of this study suggest that T. gondii infection is not rare in farmed canids.
Subject(s)
Toxoplasmosis, Animal/diagnosis , Animals , Base Sequence , DNA Primers , Foxes , Polymerase Chain Reaction , Prevalence , Raccoon Dogs , Toxoplasmosis, Animal/epidemiologyABSTRACT
Transcription is the main step in the regulation of gene expression. To study this process in vitro, it is necessary to obtain highly purified RNA polymerases. Here, we describe a method of RNA polymerase purification using a Mono Q FPLC column. Using Mono Q column chromatography accelerates the purification process and separates RNA polymerase II from RNA polymerase III with good yield.
