Resistance of rat bone marrow mesenchymal stromal precursor cells to anoxia in vitro.

We studied the effect of significantly reduced oxygen content (0% O(2)in gaseous phase) in culture medium on rat bone marrow mesenchymal stromal precursor cells. The cells retained their morphology and expression of characteristic markers during several days under these conditions; they were viable, proliferated, and some cultured cells were even capable of realizing the initial stages of osteogenic and adipogenic differentiation. Further culturing of precursor cells under conditions of anoxia led to activation of apoptosis in cultures and to progressive necrosis.

[Effect of argon and nitrogen on the peritoneal macrophages in mice and their resistance to the UV damaging effect in vitro]. / Vliianie argona i azota na peritoneal'nye makrofagi myshei i ikh ustoichivost' k povrezhdaiushchemu vosdeistviiu UF-oblucheniia in vitro.

Explored were effects of argon and nitrogen on intracellular pH in peritoneal macrophages in mice and resistance of cellular membranes to the UV damaging effect in vitro. Blasting argon or nitrogen along the surface of cell cultures in airtight chamber for 20 minutes was shown to decrease 5-folds the oxygen content of solution as compared with initial level with culture pH unchanged. Ten-minute blasting argon or nitrogen through the incubation chamber slightly elevates intracellular pH in macrophages. The standard cell incubation conditions recovered following approximately 60 minutes in hypoxic atmosphere, the ability of macrophages to build up fluorescein was degraded and they increased intracellular pH no matter the indifferent gas yet more marked in case of nitrogen in use. It was demonstrated that the normobaric gas environment with oxygen partly replaced by nitrogen or argon protects plasmatic membranes of cells from UV-induced damage.