ABSTRACT
BACKGROUND Autologous blood-derived products can target specific inflammatory molecular pathways and have potentially beneficial therapeutic effects on inflammatory pathologies. The purpose of this study was to assess in vitro the anti-inflammatory and anti-catabolic potential of an autologous blood product as a possible treatment for COVID-19-induced cytokine storm. MATERIAL AND METHODS Blood samples from healthy donors and donors who had recovered from COVID-19 were incubated using different techniques and analyzed for the presence of anti-inflammatory, anti-catabolic, regenerative, pro-inflammatory, and procatabolic molecules. RESULTS The highest concentrations of therapeutic molecules for targeting inflammatory pathways were found in the blood that had been incubated for 24 h at 37°C, whereas a significant increase was observed after 6 h of incubation in blood from COVID-19-recovered donors. Beneficially, the 6-h incubation process did not downregulate anti-COVID-19 immunoglobulin G concentrations. Unfortunately, increases in matrix metalloproteinase 9, tumor necrosis factor alpha, and interleukin-1 were detected in the product after incubation; however, these increases could be blocked by adding citric acid, with no effect on the concentration of the target therapeutic molecules. Our data allow for safer and more effective future treatments. CONCLUSIONS An autologous blood-derived product containing anti-inflammatory and anti-catabolic molecules, which we term Cytorich, has a promising therapeutic role in the treatment of a virus-induced cytokine storm, including that associated with COVID-19.
Subject(s)
Anabolic Agents/blood , Anti-Inflammatory Agents/blood , COVID-19/complications , Cytokine Release Syndrome/drug therapy , Adult , Anabolic Agents/isolation & purification , Anabolic Agents/therapeutic use , Anti-Inflammatory Agents/isolation & purification , Anti-Inflammatory Agents/therapeutic use , COVID-19/blood , Cytokine Release Syndrome/etiology , Female , Humans , Interleukin-1beta/antagonists & inhibitors , Male , Matrix Metalloproteinase 9/metabolism , Metabolism/drug effects , Middle Aged , Young Adult , COVID-19 Drug TreatmentABSTRACT
OBJECTIVE: To present the clinical management of inguinal disruption in a professional hockey player and highlight the importance of a multidisciplinary approach to diagnosis and management. CLINICAL FEATURES: A professional hockey player with recurrent groin pain presented to the clinic after an acute exacerbation of pain while playing hockey. INTERVENTION: The patient received a clinical diagnosis of inguinal disruption. Imaging revealed a tear in the rectus abdominis. Management included two platelet-rich plasma (PRP) injections to the injured tissue, and subsequent manual therapy and exercise. The patient returned to his prior level of performance in 3.5 weeks. DISCUSSION: This case demonstrated the importance of a multidisciplinary team and the need for advanced imaging in athletes with groin pain. SUMMARY: Research quality concerning the non-surgical management of inguinal disruption remains low. This case adds evidence that PRP, with the addition of manual therapy and exercise may serve as a relatively quick and effective non-surgical management strategy.
OBJECTIF: Présenter la prise en charge clinique d'une perturbation inguinale chez un joueur de hockey et mettre en évidence l'importance que revêt une approche multidisciplinaire pour le diagnostic et la prise en charge. CARACTÉRISTIQUES CLINIQUES: Un joueur de hockey professionnel souffrant d'une douleur récurrente au niveau de l'aine s'est présenté à la clinique à la suite d'une exacerbation aiguë de la douleur survenue au cours de la pratique de son sport. INTERVENTION: Le patient a reçu un diagnostic clinique d'une perturbation inguinale. L'imagerie médicale a révélé la présence d'une déchirure au niveau du grand droit de l'abdomen. La prise en charge comprenait deux injections de plasma riche en plaquettes (PRP) dans le tissu lésé, ainsi qu'une thérapeutique manuelle et des exercices ultérieurs. Le patient a retrouvé son niveau de performance antérieur en 3,5 semaines. DISCUSSION: Ce cas prouve l'importance de recourir à une équipe pluridisciplinaire et la nécessité d'utiliser des technologies d'imagerie de pointe chez les athlètes souffrant de douleurs récurrentes au niveau de l'aine. RÉSUMÉ: La qualité des recherches relatives à la prise en charge non-chirurgicale des perturbations inguinales demeure faible. Ce cas est une preuve supplémentaire que le PRP, associé à une thérapeutique manuelle et à des exercices, peut constituer une stratégie de prise en charge non-chirurgicale relativement rapide et efficace.
