ABSTRACT
OBJETIVO: Avaliar o efeito da Endopleura uchi na esteatose hepática não alcoólica em ratos obesos. MÉTODOS: Foi realizada a indução da dislipidemia por meio do tratamento com ração à base de banha de porco. Foram utilizados 12 ratos machos pesando, em média, 250g. Esses animais foram divididos em dois grupos, com seis animais cada, formando o Grupo Controle e o Grupo Uxi, tratado com Endopleura uchi. Foram analisados ganho de massa corporal, consumo de ração, concentração plasmática de colesterol, triglicerídeos, HDL-c, LDL-c, VLDL, glicose, TGO e TGP. RESULTADOS: Os grupos que consumiram o chá de uxi tiveram redução significativa no ganho de massa corporal (75,9%), bem como na esteatose macro e microvesicular, sendo observada diminuição de 50% do grau da esteatose do grupo que ingeriu o chá de uxi. Além disso, nossos resultados também apresentaram redução da balonização hepatocelular e do infiltrado inflamatório, além do grau de fibrose presente em alguns casos. CONCLUSÃO: O chá de Endopleura uchi não alterou significativamente as concentrações das aminotransferases, mas reduziu significativamente os parâmetros histológicos relacionados à esteatose não alcoólica, além de redução no colesterol total, LDL.(AU)
OBJECTIVE: To evaluate the effect of "Endopleura uchi" in nonalcoholic hepatic steatosis in obese mice. METHODS: The induction of dyslipidemia was performed through treatment with a pork lard-based diet. Twelve male rats weighing 250 g on average were used. These animals were divided into 2 groups of 6 animals each, forming the Control Group and the Uxi group, treated with Endopleura uchi. The body mass gain, feed intake, plasma concentration of cholesterol, triglycerides, HDL-C, LDL-C, VLDL, glucose, AST, and GTP were analyzed. RESULTS: The groups using Uxi tea had a significant reduction in body mass gain (75.9 %), and a reduction in macro- and microvesicular steatosis, with a 50% reduction in the degree of steatosis being observed in the group that ingested Uxi tea. Furthermore, our results also showed a reduction in hepatocellular ballooning and inflammatory infiltrate, and in the degree of fibrosis present in some cases. CONCLUSION: The tea "Endopleura uchi" did not significantly alter the concentrations of aminotransferases; but it reduced the histological parameters related to nonalcoholic steatosis significantly, and reduced total cholesterol, LDL.(AU)
Subject(s)
Animals , Rats , Fatty Liver , Phytotherapy/methods , Obesity/complications , Rats, WistarABSTRACT
Cubiu is a vegetable of Solanaceae family, native to the Amazon, which is widely distributed through Brazil, Peru, and Colombia. It is used in food, medicine, and cosmetics by native populations. Research has shown that cubiu extracts have antioxidant activities with great biological relevance. We performed a phytochemical screening to identify the main chemical groups that could confer antioxidant activity to this extract. Several tests and qualitative precipitation specific staining for major classes of secondary metabolites were used. Antioxidant capacity in vitro tests (DPPH and ABTS) were also used to assess the extract's ability to sequester free radicals of 70% hydroethanolic and aqueous extracts of cubiu flour. Alkaloids, organic acids, phenols, flavonoid glycosides, and coumarins were found in the hydroethanolic extract while the aqueous extract presented anthocyanins, gums, tannins and mucilage, amino groups, and volatile and fixed acids. For in vitro tests, the IC50 value obtained in the DPPH assay was 606.3 ± 3.5 µg/mL while that for the ABTS assay was 290.3 ± 10.7 µg/mL. Although cubiu extracts present chemical compounds directly related to antioxidant activity, our results show that it has a low antioxidant activity. Additional studies will be needed to isolate and characterize specific compounds to further assess antioxidant activity.
