Convergent and environmentally associated chromatic polymorphism in Bryconops Kner, 1858 (Ostariophysi: Characiformes: Iguanodectidae).

Bryconops Kner, 1858, includes two well defined subgenera based on morphological evidence, with each containing at least one species (B. (Bryconops) caudomaculatus and B. (Creatochanes) melanurus) with a very wide distribution, within which regional populations present color variations. To test if phenotypic variation is related to cladogenetic events, we performed tests for phylogenetic independence and determined the strength of convergence for color characters in relation to water type, as the variation between clear, black and white waters is considered to be one of the major driving forces in the evolution of Amazonian fishes. Color characters for fins above the median line of the body were generally found to be independent from phylogeny and the Wheatsheaf test strongly supports convergence of the dorsal fin color between populations of species in the same type of water, with a similar trend suggested for the color of the dorsal lobe of the caudal fin. This means that simple color characters cannot necessarily be relied upon for taxonomic revisions of the genus as local phenotypic variants may represent environmentally determined plasticity or convergent evolution. Further studies are required to determine the validity of these characters.

Revisiting the phylogeny of the genus Lolliguncula Steenstrup 1881 improves understanding of their biogeography and proves the validity of Lolliguncula argus Brakoniecki & Roper, 1985.

The biogeography of American loliginid squids has been improved in recent years, but certain key taxa have been missing. Given that the most accurate phylogenies and estimates of divergence times of common ancestors depend heavily on good taxonomic coverage we have reanalyzed the genus Lolliguncula in light of new samples that increase the geographic and taxonomic coverage. New sequences were produced using standard methods to update an existing dataset for COI, 16S and Rhodopsin markers. Data was analyzed using various species delimitation methods, rigorous phylogenetic analyses and estimates of divergence times between clades. Within Lolliguncula we recover five monophyletic lineages that relate to the known species L. argus, L. diomedeae, L. panamensis, L. brevis North Atlantic and L. brevis South Atlantic. Except when using low divergence thresholds in ABGD, species delimitation methods only identify four of these lineages as distinct species, grouping L. argus and L. diomedeae as a single species. However, considering the reciprocal monophyly, recent divergence time estimate and morphological diagnoses we refrain from synonymizing L. argus within L. diomedeae, considering them very recently diverged species. The biogeography of the American loliginids is discussed, wherein basal cladogenesis in both Lolliguncula and Doryteuthis occur between the Atlantic and Pacific about 45 mya, with subsequent speciation around 20 mya associated with seafloor changes during the formation of the Caribbean. The recent speciation between L. argus and L. diomedeae is associated to oceanic environmental changes associated with glaciation, deep sea cooling and tropical upwelling.