ABSTRACT
Mycobacterium vaccae exposed to compounds which are known to disorganise the cell wall composition and architecture (protamine, glycine) showed increased specific activity in beta-sitosterol biotransformation to androstene derivatives, intennediates in the production of most medical steroids. GC/MS analysis of free lipid fatty acids revealed higher content of unsaturated compounds, mainly C16:1 and C18:1 in protamine- and glycine-treated cells than that in control cells, which seems to change the permeability features of the cell wall barrier, facilitating hydrophobic beta-sitosterol diffusion.
Subject(s)
Mycobacterium/metabolism , Sitosterols/metabolism , Androstadienes/metabolism , Androstenedione/biosynthesis , Biotransformation , Cell Membrane Permeability/drug effects , Cell Wall/metabolism , Fatty Acids/metabolism , Glycine/pharmacology , Mycobacterium/drug effects , Protamines/pharmacologyABSTRACT
The aim of the study was to investigate the effect of a new selective alpha 1-adrenoreceptor blocker doxazosin on blood pressure, serum lipids and lipoproteins in patients with essential hypertension. The study was done in 32 out-patients with mild-to-moderate hypertension (22 men and 10 women, mean age 45.6 +/- 10.1). After 2-week placebo period the patients were given doxazosin in increasing doses from 1 to 8 mg daily for 6 to 14 weeks (mean daily dose 2.24 +/- 1.6 mg). Twenty-nine patients completed the study. The active treatment caused highly significant drop in systolic and diastolic blood pressure both in supine and standing positions. No orthostatic hypertension was noted. There was also a statistically significant decrease in serum total cholesterol, VLDL-cholesterol, and triglyceride levels and increase in the positive prognostic lipid indicators. HDL3-cholesterol and apolipoprotein AI levels as well as HDL/total cholesterol ratio. Accordingly, the statistically significant decrease of the so called atherogenic index was noted. The drug was well tolerated and only one patient dropped from the study because of side effects. The authors conclude that doxazosin appears to be an effective and well tolerated antihypertensive drug with a favorable effect on lipid metabolism. It may be particularly useful in hypertensive patients with coexisting lipid abnormalities.
Subject(s)
Doxazosin/therapeutic use , Hypertension/drug therapy , Lipids/blood , Adult , Aged , Female , Humans , Hyperlipidemias/complications , Hyperlipidemias/prevention & control , Hypertension/complications , Hypertension/metabolism , Male , Middle AgedSubject(s)
Glycosaminoglycans/urine , Mucopolysaccharidosis I/urine , Child , Child, Preschool , Female , Humans , MaleABSTRACT
The purpose of this work was to analyse amino-acids in urine of mentally handicapped children and concentration of amino-acids in urine. Undesalted, night urine was investigated by two dimensional ascending chromatography method. Preliminary results show a great variety of composition from 6 to 23 amino-acids and pathological amino-acids excretion in 14% cases. Besides normal presented amino-acids in urine it was till now detected 3.5% cases of phenylketonuria, 1.5% cases of prolinuria and 24% cases of amino-butyric-acids excretion.
