Ferromagnetic-like behavior of Co doped TiO2 flexible thin films fabricated via co-sputtering for spintronic applications.

TiO2:Co thin films on ITO (Indium-tin-oxide)/PET (Poly Ethylene Terephthalate) flexible and glass substrates were fabricated via DC magnetron co-sputtering at room temperature. The samples deposited on glass substrates were subjected to annealing processes at 473 K for 2 h to improve the crystallization of the material. Both TiO2:Co/ITO/PET and TiO2:Co/glass thin films exhibited excellent optical properties with more than 80% transmission in the visible region. An increase on ITO/PET surface temperature was detected during the synthesis of the samples; this variation in the ITO substrate ( ∼ 2 K) was associated to impact energetic of ion or atoms bombarded during the deposition process. X-ray diffraction measurements evidenced local phases to growth on the flexible substrate; the random distributions of Cobalt crystals into the rutile and anatase phases were associated to a crystalline lattice embedded with magnetic ions. A configuration of small grains and absence of cluster formation on the surface of thin films was observed through SEM and AFM measurements. From this topographic study and MFM measurements evidenced that surface grains were not constituted magnetic domains formation in the thin films. The ferromagnetic-like behavior was observed in a magnetization as function of field measurement by PPMS. M vs H curves at room temperature for TiO2:Co/ITO/PET thin films, showed the hysteresis loop. The dipolar interaction between Cobalt ions without formation of domains were correlated to the magnetic behavior in the material, as the doping concentration is lower than 12%.

Female reproductive traits of a commercially exploited skate: Atlantoraja platana (Günther, 1880) (Chondrichthyes, Rajidae). Ovarian morphology, gametogenesis and microscopic verification of maturity criteria.

Atlantoraja platana is an endemic species of the Southwest Atlantic Ocean, and is one of the most captured by the local bottom trawl industrial fisheries. In this work, the microscopic architecture of mature female's gonads and the dynamics of follicle development are studied as a contribution to raise awareness of reproductive biology of the species. Folliculogenesis depicts the same histologic pattern as in other Elasmobranchs. Follicles in different degrees of maturation coexist in mature animals. The oogonia were only found in immature individuals. Likewise, atretic follicles were recorded in ovaries of all sexual maturity stages. The microscopic size recorded from the beginning of yolk input is smallest than the detected with the necked eye. This study provides valuable information about female's gametogenesis that could be taken into account in the development of fisheries management.

A comparative morphological study of the epiandrous apparatus in mygalomorph spiders (Araneae, Mygalomorphae).

Many adult male spiders have silk glands which are not associated with the spinnerets. They occur on the anterior margin of the genital furrow and are used during the building of the sperm web and sperm droplet induction. These epiandrous glands exit the body through ducts which lead to specialized spigots. In the taxon Mygalomorphae, the presence of epiandrous spigots is just reported for a couple of species but their morphology has not been investigated. In this paper we provide a detailed study of the ultrastructure morphology of eighteen species belonging to eight families using light and scanning electron microscopy (SEM). We describe and present images of the epiandrous spigots above the genital opening. Also, we compare the morphology of spigots between families and describe the epiandrous glands through histology.

Morphological indicators of initial reproductive commitment in Mustelus schmitti (Springer 1939) (Chondrichthyes, Triakidae): folliculogenesis and ovarian structure over the life cycle.

This work provides information about the sexual commitment and the folliculogenesis of the gatuzo, Mustelus schmitti. A total of 112 females of all maturity stages were fished in the Bahía Blanca estuary, between 2009 and 2010. The oogonia were present throughout the life cycle of the animals. The folliculogenesis follows a pattern similar to other elasmobranchs. The granulosa layer keeps monolayered throughout the folliculogenesis, but with two cell types in the vitellogenic follicle. The zona pellucida forms in the primordial follicles. The thecal system shows a connective inner layer and a glandular outer sheath. The microscopic beginning of the sexual commitment, indicated by the vitello hoarding, takes place in follicles from 500 micrometres, while the macroscopic evidence appears in follicles of 2500-3000 micrometres. The results presented in this study suggest that the fishery pressure may affect a susceptible range of sizes of the species, not previously considered and provides a biological framework for the development of fisheries policy.

Dilatación unilateral completa de conducto deferente como causa de masa inguinal / Complete unilateral dilation of the vas deferens as a cause of an inguinal mass

La dilatación completa unilateral del conducto deferente es un hallazgo radiológico extremadamente raro. La mayoría de las estructuras quísticas adyacentes a la próstata se pueden agrupar en quistes y divertículos. El hallazgo de masas inguinales obliga a descartar la existencia, entre otras entidades, de hernia intestinal. Presentamos el caso de un paciente con masa inguinal secundaria a conducto deferente dilatado de manera unilateral (AU)

The complete unilateral dilation of the vas deferens is an extremely rare radiologic finding. Most cystic structures adjacent to the prostate can be grouped into cysts and diverticula. The finding of an inguinal mass makes it necessary to rule out intestinal hernias and other entities. We present the case of a patient who developed an inguinal mass secondary to unilateral dilation of the vas deferens (AU)

[Complete unilateral dilation of the vas deferens as a cause of an inguinal mass]. / Dilatación unilateral completa de conducto deferente como causa de masa inguinal.

The complete unilateral dilation of the vas deferens is an extremely rare radiologic finding. Most cystic structures adjacent to the prostate can be grouped into cysts and diverticula. The finding of an inguinal mass makes it necessary to rule out intestinal hernias and other entities. We present the case of a patient who developed an inguinal mass secondary to unilateral dilation of the vas deferens.

Ovarian folliculogenesis in the smallnose fanskate sympterygia bonapartii (Müller & Henle, 1841) (Chondrichthyes, Rajidae) / Foliculogénesis ovárica en la raya marmorada, sympterygia bonapartii (Müller & Henle, 1841) (Chondrichthyes, Rajidae)

Chondrichthyes have become an important economic resource in the last decades, with Argentina as one of the countries that exploits more sharks and skates, even at levels that exceed de limits of many species. However, there is a scarce knowledge of the reproductive biology of this group, particularly from species inhabiting the Southern hemisphere. This work shows the most relevant facts during folliculogenesis in Sympterygia bonapartii. Results show that germinal cells are present in immature and maturing females. The most important facts that vary along de follicular development are the number of types and layers of follicular cells, the establishment of thin projections from the follicular cells and the degree of development of the thecae. Follicular cells are, at least, of two different types and both of them emit projections that break through the zona pellucida. The outer theca shows signs of synthetic activity. Atretic follicles of different sizes are present in exemplars of all the reproductive stages. These results are discussed in a physiological and adaptive context.

Los Condrictios se han convertido en un recurso económico importante en las últimas décadas, siendo Argentina uno de los países que más explota tiburones y rayas, incluso a niveles que exceden los límites de varias especies. A pesar de esto, es poco lo que se conoce sobre la biología reproductiva de este grupo, particularmente en especies del Hemisferio Sur. En este trabajo se estudian los estadios más relevantes de la foliculogénesis en Sympterygia bonapartii. Los resultados muestran que las ovogonias están presentes tanto en ejemplares inmaduros como subadultos. Las características más importantes que varían a lo largo del desarrollo folicular son el número de capas y tipos celulares que constituyen el epitelio folicular, el desarrollo de proyecciones de las células de la granulosa y el grado de desarrollo de las tecas. Las células foliculares son, al menos, de dos tipos y ambos emiten proyecciones que atraviesan la zona pelúcida. La teca externa presenta características compatibles con la actividad sintética. Folículos atrésicos de distintos tamaños están presentes en ejemplares de todos los estadios de madurez sexual. Estos resultados se discuten en un marco fisiológico y adaptativo.

Morphological changes in the pregnant uterus of the smooth hound dogfish mustelus schmitti Springer, 1939 (Gatuzo) (Condrichthyes, Triakidae): microscopic study and phylogenetic reproductive implications / Cambios morfológicos en el útero gestante del gatuzo mustelus schmitti Springer, 1939 (Chondrichthyes, Triakidae): estudio histológico e implicaciones filogenéticas y reproductivas

Elasmobranchs are important, not only as an economic resource but also for their phylogenetic position. Their slow growth and long reproductive cycles, together with the high fisheries pressure exerted, attempt to the knowledge of all biological parameters of species. In this work we analyze the histology of the pregnant uterus of the gatuzo, a viviparous aplacental shark. The mucosa depicts modifications similar to those of placental species and others proper of histotrophism. Likewise, a network-like muscular layer in the mucosa was described for first time in the genus. The presence of vitelline plates in the gut of at term embryos suggests that the maternal supplies are not enough for the big fetus development. The relationships between maternal support and embryo growth are discussed into the matrotrophic and phylogenetic contexts.

Los peces cartilaginosos son importantes tanto desde el punto de vista evolutivo como desde el comercial, ya que integran una parte importante de las pesquerías argentinas. El gatuzo es explotado intensamente, pero se desconocen todos los parámetros biológicos necesarios para establecer medidas adecuadas de manejo sustentable. En este trabajo se estudia la arquitectura del útero gestante de esta especie vivípara aplacentaria. Se observaron cambios estructurales en el epitelio uterino así como secreción mucosa y descamación del mismo. Asimismo se describe una túnica muscular reticuliforme en la mucosa, que no había sido referida para ningún otro elasmobranquio. Se aportan evidencias de un histotrofismo moderado. Se observó la presencia de vitelo en la válvula espiral de los embriones gestantes, lo que junto con la secreción uterina, contribuye al desarrollo de los fetos. Se discute la importancia del aporte materno en el crecimiento embrionario, desde el punto de vista de los diferentes tipos de matrotrofismo.

Morphological study of the oviductal gland in the smallnose fanskate Sympterygia bonapartii (Müller and Henle, 1841) (Chondrichthyes, Rajidae).

The oviductal gland is an exclusive structure of cartilaginous fishes that produces the egg jelly, forms the tertiary egg envelopes and stores sperm. The biological importance of this structure is related to the special features of the reproductive strategy of the group and to its phylogeny, considering that egg-laying is the ancestral condition in this fish (Dulvy and Reynolds, 1997). This gland of the smallnose fanskate shows four morphofunctional zones. The lining epithelium along the gland is columnar with secretory and ciliated cells. Secretions are mucous and/or proteic according to the zone, and to their specific functions. This is the first report about the microanatomy of the female reproductive tract of S. bonapartii with evidence of sperm storage in the genus.

The ovary of the bignose fanskate Sympterygia acuta Garman, 1877 (Chondrichthyes, Rajidae) in the Bahía Blanca estuary, Argentina: morphology and reproductive features.

Chondrichthyes have become an important economic resource in recent years. In spite of this importance, there is little knowledge about their reproductive biology, especially of species from the south-western Atlantic. In this work, we study the morphology and histology of the ovary of Sympterygia acuta. The results show that oogonia were present only in the immature females (stage I of maturity scale), whereas specimens in stages II and III of maturity displayed oocytes in all developmental steps. The most important histological features that vary throughout oocyte development are the presence or absence of yolk, the number of types and layers of follicular cells and the degree of development of the thecae. Follicular cells are, at least, of two different types. Finally, a new point of view for the determination of maturity stages in the field based on the size of follicles is discussed.

The ovary of the bignose fanskate Sympterygia acuta Garman, 1877 (Chondrichthyes, Rajidae) in the Bahía Blanca estuary, Argentina: morphology and reproductive features / O ovário da bignose fanskate Sympterygia acuta Garman, 1877 (Chondrichthyes, Rajidae) no estuário de Bahía Blanca, Argentina: morfologia e características reprodutivas

Chondrichthyes have become an important economic resource in recent years. In spite of this importance, there is little knowledge about their reproductive biology, especially of species from the south-western Atlantic. In this work, we study the morphology and histology of the ovary of Sympterygia acuta. The results show that oogonia were present only in the immature females (stage I of maturity scale), whereas specimens in stages II and III of maturity displayed oocytes in all developmental steps. The most important histological features that vary throughout oocyte development are the presence or absence of yolk, the number of types and layers of follicular cells and the degree of development of the thecae. Follicular cells are, at least, of two different types. Finally, a new point of view for the determination of maturity stages in the field based on the size of follicles is discussed.

Os Chondrichthyes tornaram-se num importante recurso econômico nos últimos anos. Apesar da sua importância, é muito pouco o que se conhece da sua biologia reprodutiva, especialmente das espécies do Atlântico sul-ocidental. Neste trabalho, foi estudada a morfologia e histologia do ovário de Sympterygia acuta. Os resultados mostraram que as oogonias somente encontraram-se presentes nas fêmeas imaturas (fase I de escala de maturidade) e que os animais em estágio II e III de maturidade exibiram oocitos em todas as etapas de desenvolvimento. As características histológicas mais importantes que variam ao longo de desenvolvimento dos oocitos são a presença da suarda, o tipo e quantidade de celas foliculares e o grau do desenvolvimento do thecae. Existem pelo menos dois tipos de células foliculares. Finalmente se discute um novo ponto de vista para a determinação dos estágios de maturidade no campo, baseado no tamanho dos folículos.

Histological remarks of the oviduct and the oviducal gland of Sympterygia acuta Garman, 1877.

The elasmobranchs constitute an important resource in Argentinian fishing and they show reproductive characteristics that make them susceptible to the pressure of fishing. In spite of the importance of the resource our knowledge about the species of the Southwestern Atlantic is scarce. In this work we study the microanatomy of the oviduct and the oviducal gland of Sympterygia acuta. The results show a very folded oviduct with mucous and ciliated cells. The oviducal gland depicts the same zonation as other batoids, showing the typical four regions (club, papillary, baffle and terminal). The epithelium lining the glandular lumen is simple columnar with ciliated and glandular cells. The four zones show simple or ramified tubular gland the secretions of which constitute the egg's envelopes. The club and baffle zones are similar to those present in other species. The papillary one depicts a different pattern of secretions that other Rajiformes and the last zone of the gland is characterized by mixed adenomers. The information here exposed constitutes the first report on the microanatomy of the genital tract of S. acuta.

Histological remarks of the oviduct and the oviducal gland of Sympterygia acuta Garman, 1877 / Observações histológicas do oviduto e da glândula oviducal de Sympterygia acuta Garman, 1877

The elasmobranchs constitute an important resource in Argentinian fishing and they show reproductive characteristics that make them susceptible to the pressure of fishing. In spite of the importance of the resource our knowledge about the species of the Southwestern Atlantic is scarce. In this work we study the microanatomy of the oviduct and the oviducal gland of Sympterygia acuta. The results show a very folded oviduct with mucous and ciliated cells. The oviducal gland depicts the same zonation as other batoids, showing the typical four regions (club, papillary, baffle and terminal). The epithelium lining the glandular lumen is simple columnar with ciliated and glandular cells. The four zones show simple or ramified tubular gland the secretions of which constitute the egg's envelopes. The club and baffle zones are similar to those present in other species. The papillary one depicts a different pattern of secretions that other Rajiformes and the last zone of the gland is characterized by mixed adenomers. The information here exposed constitutes the first report on the microanatomy of the genital tract of S. acuta.

Os elasmobranchii constituem um importante recurso pesqueiro na Argentina e mostram características reprodutivas que os fazem suscetíveis à pressão de pesca. Apesar da importância do recurso, nosso conhecimento sobre as espécies do Atlântico do Sudoeste é escasso. Neste trabalho, estudamos a microanatomia do oviduto e da glândula oviducal de Sympterygia acuta. Os resultados mostram um oviduto muito dobrado, com células mucosas e células ciliadas. A glândula oviducal apresenta as mesmas zonas que outros batóideos, mostrando as quatro regiões típicas (club, papillary, baffle e terminal). O epitélio que reveste o lúmen glandular é simples, com células ciliadas e células glandulares. As quatro zonas mostram glândulas tubulares simples ou ramificadas e suas secreções constituem os envelopes do ovo. As zonas do club e baffle são semelhantes aquelas presentes em outras espécies. A papilar tem um padrão de secreção diferente de outros Rajiformes e a última zona da glândula é caracterizada por adenômeros mistos. A informação aqui exposta constitui o primeiro relato sobre a microanatomia do trato genital de S. acuta.

The spleen of Zaedyus pichiy, (Mammalia, Dasypodidae): a light and electron microscopic study.

Armadillos are ancient mammals important as models for biomedical, evolutive and ecological studies, because they have adaptive and primitive morpho-physiological characteristics. In this work we study the splenic microarchitecture of the 'Patagonian Piche', Zaedyus pichiy, as an attempt to understand the relationship between the organizational plan of the organ and the particular features of this species. The organ samples were classically processed for light and electron microscopic study. The microanatomy of the organ as well as its different cell types are studied. The spleen is non-sinusoidal, with the typical arrangement for storage functions. White pulp is well defined. Red pulp is a meshwork of circulating, immunocompetent and haemopoietic cells. The general structure of the organ agrees with the semi-fossorial habit and the adaptability of the species. Comparative aspects with other armadillos or other less specialized groups are discussed. Persistence of haemopoietic spleen activity in the adult suggests the existence of specific inductive functions of the stroma. Better knowledge of spleen structure and function in ancient mammals may give important information about their phylogeny.

Ultrastructure and morphometry of ovarian follicles in the armadillo Chaetophractus villosus (Mammalia, Dasypodidae).

Ultrastructural and morphometric changes in oocyte and surrounding granulosa cells during armadillo follicular growth are described. Primordial, intermediary, early and late primary, secondary, tertiary and preovulatory Graafian follicles were found. From primordial to Graafian follicle, granulosa cells increase in height, become multilayered while fluid-filled spaces arise among them in the tertiary follicle stage. As the follicle expands the oocyte is located eccentrically and grows in size. From primordial to secondary stage organelles develop in number and complexity and many vesicles start arising in the cytoplasm at the secondary stage. From tertiary follicle stage the number of mitochondria decreases. In follicular cells, secreting-related organelles expand, but mitochondrial number decreases according to the follicle expansion. Intercellular junctions between both cell types increase. The morphometrical study shows a biphasic pattern of oocyte and follicle growth. The morphological baseline here provided will be essential for further comprehension of the reproductive biology in armadillos.

Ultrastructure and morphometry of ovarian follicles in the armadillo Chaetophractus villosus (Mammalia, Dasypodidae)

Ultrastructural and morphometric changes in oocyte and surrounding granulosa cells during armadillo follicular growth are described. Primordial, intermediary, early and late primary, secondary, tertiary and preovulatory Graafian follicles were found. From primordial to Graafian follicle, granulosa cells increase in height, become multilayered while fluid-filled spaces arise among them in the tertiary follicle stage. As the follicle expands the oocyte is located eccentrically and grows in size. From primordial to secondary stage organelles develop in number and complexity and many vesicles start arising in the cytoplasm at the secondary stage. From tertiary follicle stage the number of mitochondria decreases. In follicular cells, secreting-related organelles expand, but mitochondrial number decreases according to the follicle expansion. Intercellular junctions between both cell types increase. The morphometrical study shows a biphasic pattern of oocyte and follicle growth. The morphological baseline here provided will be essential for further comprehension of the reproductive biology in armadillos.

São descritas a ultra-estrutura e as mudanças morfométricas nos ovócitos e nas células da granulosa durante o crescimento folicular do tatu. Foram encontrados folículos primordiais, intermediários, primários (iniciais e finais), secundários, terciários e folículos de Graff pré-ovulatórios. Desde os folículos primordiais até os folículos de Graff, as células da granulosa aumentam de tamanho, dispõem-se em múltiplas camadas e, no folículo terciário, apresentam espaços cheios de líquido entre elas. Quando o folículo se expande, o ovócito localiza-se exentricamente e aumenta de tamanho. Nos folículos primários e secundários, as organelas desenvolvem-se aumentando em número e complexidade e, no folículo secundário, várias vesículas desenvolvem-se no citoplasma. A partir do folículo terciário, o número de mitocôndrias diminui. Nas células foliculares, as organelas relacionadas à secreção se expandem, mas o número de mitocôndrias diminui com a expansão do folículo. As uniões intercelulares entre ambos os tipos de células aumentam. Os estudos morfométricos mostram um padrão bifásico de crescimento do ovócito e do folículo. A informação morfológica deste trabalho pode ser de grande importância no entendimento da biologia reprodutiva dos tatus.

Ultrastructure and morphometry of ovarian follicles in the armadillo Chaetophractus villosus (Mammalia, Dasypodidae)

Ultrastructural and morphometric changes in oocyte and surrounding granulosa cells during armadillo follicular growth are described. Primordial, intermediary, early and late primary, secondary, tertiary and preovulatory Graafian follicles were found. From primordial to Graafian follicle, granulosa cells increase in height, become multilayered while fluid-filled spaces arise among them in the tertiary follicle stage. As the follicle expands the oocyte is located eccentrically and grows in size. From primordial to secondary stage organelles develop in number and complexity and many vesicles start arising in the cytoplasm at the secondary stage. From tertiary follicle stage the number of mitochondria decreases. In follicular cells, secreting-related organelles expand, but mitochondrial number decreases according to the follicle expansion. Intercellular junctions between both cell types increase. The morphometrical study shows a biphasic pattern of oocyte and follicle growth. The morphological baseline here provided will be essential for further comprehension of the reproductive biology in armadillos

Evaluación de la inmunogenicidad de la vacuna recombinante contra la hepatitis viral B (HVB) en una población infantil en alto riesgo / Evaluation of the inmunogenecidad of the recombinant vaccine against the B hepatitis (HBV) in a poblation infantile in high risk

La hepatitis viral B continúa siendo una de las patologías hepáticas más frecuentes; en ese sentido, sólo en el continente americano se estima que hay cerca de 7 millones de personas infectadas. Esta situación se ve agravada por las condiciones socio-sanitarias de los países en desarrollo, así como por las deficiencias en los programas de prevención, tales como la vacunación universal en niños menores de un año y en poblaciones en alto riesgo. El propósito del presente trabajo fue el de inmunizar contra la hepatitis viral B a una población de niños en alto riesgo, en edades entre un 1 mes a 10 años, con una vacuna recombinante ya aprobada en Venezuela. Para tal fin, se elaboró un protocolo abierto, multicéntrico, donde se incluyeron 244 niños (138 varones y 106 hembras), aparentemente sanos, seronegativos para la hepatitis viral B (HBsAg y Core Total), no vacunados previamente contra la hepatitis viral B, provenientes de "hogares" del Instituto Nacional del Menor. A los niños se les tomó una muestra de sangre (3,0 ml) de la vena

Spleen of Dasypus hybridus (Mammalia, dasypodidae): a light and electron microscopic study.

Armadillos are relictual mammals important as models for biomedical studies. They contain adaptative and primitive characteristics in both anatomical and physiological aspects. In this study we describe the splenic histology and cytology of the "mulita," Dasypus hybridus. Organ samples were processed for light and electron microscopy study. The microanatomy of the organ samples as well as their different cell types are described. The spleen is non-sinusoidal, with the typical arrangement for storage functions. White pulp is lightly diffuse. Red pulp is a meshwork of circulating, immunocompetent and hemopoietic cells. Differences with other studied members of the group are discussed. The general structure of the organ agrees with the semi-fossorial habit of the species. Persistence of myeloid activity in the adult suggests the existence of specific inductive functions of the stroma. Better knowledge of this fact may give further insight on the phylogeny of hemopoiesis.

The hemopoietic system: a phylogenetic approach.

Nomadism is a true hemopoietic characteristic during vertebrate phylogeny and ontogeny. This work reviews the mechanism and developmental steps of hemopoiesis, from a phylogenetic point of view. A summary of the principal hemopoietic "foci" along the evolutionary line is also presented.