ABSTRACT
The transcriptomes from different organs and tissues of western poplar, eucalyptus, soybean and common bean were studied. The expression level of cellulose synthase genes was notably different in different types of tissues and organs in studied plants. For common bean and eucalyptus transcriptome the domination of certain cellulose synthase genes was typical. These prevailing genes made up more than 50 % of the total expression pull of cellulose synthases. On the contrary, cellulose synthase expression pulls of wes-tern poplar and soybean were distributed between multiple genes. The different expression strategies of CesA-genes may reflect a phylogenetic processes that occurred in genomes studied.
Subject(s)
Eucalyptus/genetics , Gene Expression Regulation, Plant , Genome, Plant , Glucosyltransferases/genetics , Glycine max/genetics , Phaseolus/genetics , Plant Proteins/genetics , Populus/genetics , Arabidopsis/classification , Arabidopsis/genetics , Arabidopsis/metabolism , Eucalyptus/classification , Eucalyptus/metabolism , Fruit/genetics , Fruit/metabolism , Glucosyltransferases/metabolism , High-Throughput Nucleotide Sequencing , Isoenzymes/genetics , Isoenzymes/metabolism , Multigene Family , Organ Specificity , Phaseolus/classification , Phaseolus/metabolism , Phylogeny , Plant Leaves/genetics , Plant Leaves/metabolism , Plant Proteins/metabolism , Plant Roots/genetics , Plant Roots/metabolism , Plant Stems/genetics , Plant Stems/metabolism , Populus/classification , Populus/metabolism , Glycine max/classification , Glycine max/metabolism , Species Specificity , TranscriptomeABSTRACT
A bioinformatic search of sequences encoding cellulose synthase genes in the flax genome, and their comparison to dicots orthologs was carried out. The analysis revealed 32 cellulose synthase gene candidates, 16 of which are highly likely to encode cellulose synthases, and the remaining 16--cellulose synthase-like proteins (Csl). Phylogenetic analysis of gene products of cellulose synthase genes allowed distinguishing 6 groups of cellulose synthase genes of different classes: CesA1/10, CesA3, CesA4, CesA5/6/2/9, CesA7 and CesA8. Paralogous sequences within classes CesA1/10 and CesA5/6/2/9 which are associated with the primary cell wall formation are characterized by a greater similarity within these classes than orthologous sequences. Whereas the genes controlling the biosynthesis of secondary cell wall cellulose form distinct clades: CesA4, CesA7, and CesA8. The analysis of 16 identified flax cellulose synthase gene candidates shows the presence of at least 12 different cellulose synthase gene variants in flax genome which are represented in all six clades of cellulose synthase genes. Thus, at this point genes of all ten known cellulose synthase classes are identify in flax genome, but their correct classification requires additional research.
