[Biological age and the pain syndrome at diabetic polyneuropathy].

Patients with diabetic polyneuropathy were examined to study their biological age, rate of aging and pain syndrome. More rapid rate of aging was revealed in patients with diabetic polyneuropathy and pain syndrome. Using Duloxetin and Gabapentin is reliable to decrease the display of pain syndrome in diabetic polyneuropathy patients. Against the background of pain syndrome therapy the rate of aging is noticed to decrease along with the regression of pain syndrome. The medicines of Duloxetin and Gabapentin which are used during depression and epilepsy protect against aging.

[Efficacy and safety of cefixim and ciprofloxacin in acute cystitis (a multicenter randomized trial)].

A prospective multicenter randomized trial enrolled 104 females aged 18-55 years with acute un-complicated cystitis. The patients were randomized into two groups: 49 patients of group 1 received cefixim in a single dose 400 mg/day for 5 days; 55 patients of group 2 were given ciprofloxacin in a dose 250-500 mg twice a day for 5 days. Clinical and microbiological assessment of efficacy and safety was made before treatment and on treatment day 8 and 28. Significant differences were found between groups 1 and 2 in parameters of bacteriological and clinical efficacy. Eradication of the agent and persistent bacteriological response was seen in 95.9 and 100% patients of group 1, 66 and 100% patients of group 2, respectively. Complete and partial response was observed in 55.1 and 75.5% patients of group 1, 37.3 and 58.1% patients of group 2, respectively. Thus, cefixim in a single dose 400 mg/day for 5 days has a higher microbiological efficacy than ciprofloxacin in a dose 250-500 mg twice a day. Side effects occurred less frequently in the treatment with cefixim.

[Anthrax cattle burial grounds in Russia and their biosafety].

AIM: To develop standard operating procedure for assessment level of biohazard for population living near existing cattle burial grounds. MATERIALS AND METHODS: Development of standard operating procedure for assessment of biosafety of cattle burial grounds was performed on the basis of regulatory documents and regional field practical experience. Assessment of biohazard level of anthrax cattle burial grounds was performed in 5 regions choosen on the basis of certain criteria. Total number of samples taken was 762. RESULTS: Rating of federal districts according to number of permanently problematic on anthrax places was composed. It was determined that maximal number of permanently problematic on anthrax places was active during time period until 1950. Agent of anthrax was not detected in studied samples either as spores or vegetative forms and soil was categorized as "clean" on all indicators. CONCLUSION: It was determined that conditions of burial of cattle fallen due to anthrax and their maintenance rules are followed in 5 places. All cattle burial grounds are located inside towns' borders or adjoin to areas of existing or planned apartment blocks.

[Norfloxacine efficacy in acute cystitis in the region with 10% resistance of E. coli to fluoroquinolones: a comparative randomized study].

We have conducted a clinicomicrobiological study the first stage of which consisted in collection of information on uropathogens resistance to fluoroquinolones in 89 females with uncomplicated urinary infections (UI). Sensitivity to antibacterial drugs was determined by the agar dilution test (CLSI, 2007). At stage two of the study we made a prospective multicenter randomized trial including 108 females aged 18-55 years with acute uncomplicated cystitis. The patients were randomized into two groups: group 1 (n = 55) received norfloxacin (400 mg twice a day for 3 days); group 2 (n = 53) received phosphomycin (a single 3.0 g dose). Clinical and microbiological assessment of efficacy and safety was performed before the treatment, on treatment day 5, 10 and 28. Isolation of E. coli strains in the region highly and moderately resistant to cyprofloxacine was rather high--10 and 1.1%, respectively. Before the treatment E. coli was isolated from the urine in 82.4% cases, E. faecalis--5.9%, S. saprophyticus--3.9%, Staphylococcus spp--2.0%, others--< 1%. No significant differences by bacteriological and clinical efficacy were found between the groups. Eradication of the agent and persistent bacteriological response were seen in 100 and 95.2%, 95.8 and 100% patients of groups 1 and 2, respectively. Complete and partial responses were registered in 68.5 and 76%, 76 and 98% patients of groups 1 and 2, respectively. Thus, treatment with norfloxacin in a dose 400 mg twice a day for 3 days and with phosphomycin in a 3.0 g single dose have high clinical and microbiological efficacy in uncomplicated cystitis. Norfloxacin retains clinical and microbiological efficacy in acute uncomplicated cystitis even in conditions of 10% prevalence of fluoroquinolone-resistant strains. We think that there are reasons for revision of criteria of interpretation of uropathogens sensitivity to fluoroquinolones in the treatment of uncomplicated UI.

Some properties of alkaline DNases of tentacles of actinia Radianthus macrodactylus and their hemolytic activity.

Two alkaline DNases of tentacles of actinia Radianthus macrodactylus, referred to as alk DNase I and alk DNase II, respectively, have been purified up to apparent homogeneity with consecutive column ion exchange chromatography and gel filtration. Both enzymes have a lot of common properties, such as the ability to hydrolyze very effectively p-nitrophenyl-5'-TMP and heat-denatured DNA. They both have no preferential specificity to the sugar component of the nucleic acids and effectively digest ribopolymers. Their ability to hydrolyze supercoiled DNA of the pBR322 plasmid and linear DNA of the lambda phage by "miscellaneous" exo- and endonucleolytic types of attack and to produce nucleosides, nucleotides and short oligonucleotides suggests their similarity with phosphodiesterase I (5'-exonuclease, oligonucleate 5'-nucleotidohydrolase; E.C. 3.1.4.1), isolated from rattle snake Crotalus adamenteus venom. Alk DNase II has been revealed to have some uncommon properties, such as phosphomonoesterase and hemolytic activities. The protein causes a very potent lysis of human and rabbit erythrocytes. The ability of alk DNase II to precipitate some components of normal human and rabbit blood serum as well as the inhibition of this reaction by fucose but not by another monosaccharides suggest the enzyme to have a lectin-like activity. The appearance of only one protein band during electrophoresis of alk DNase II in denaturation conditions suggests that all activities are inherent to the same molecule of protein. The possible role of alkaline DNases in the toxic effect of burning by actinia tentacles is discussed.

[Comparative study of the quality of domestic albumin solution and foreign analogs]. / Sravnitel'noe izuchenie kachestva otechestvennogo rastvora al'bumina i zarubezhnykh analogov.

The quality and standards of albumin solutions (5, 10 and 20%) made in this country meet the requirements of national specification documentation. This, however, lacks some standards included into European Pharmacopoeia and mandatory for foreign manufacturers (Na and K ions, hemipigments, polymers, thermostability). The comparative tests of the albumin solutions made in Russia and abroad by conventional European standards showed that Russian solutions by some parameters are inferior to foreign samples. This urges improvement of the solution production technology as well as updating technical documents regulating the product quality.

[Systematic approach to transfusiology]. / Sistemnyi podkhod v transfuziologii.

Possible methods of systemic approach to transfusiology as an independent science have been considered. It has been noted that, despite a great interrelation with other branches of science, transfusiology has its theoretical basis, and therefore the methods of other sciences, when used for transfusiologic purposes, acquire a definite tendency and specificity. As an example, the authors describe the main features of the systemic approach to controlling blood and circulation with the aim of selecting optimal tactics and strategy.

[Method of determination and classification of surgical risk]. / Metod opredeleniia i klassifikatsiia khirurgicheskogo riska

The authors have elaborated their own five-grade classification of a surgical risk, based on evaluation of 2000 case reports. All patients and surgical interventions are subdivided into 5 typical groups in terms of increasing hazards inherent in a surgical intervention. The authors differentiated minimum (I), moderate (II), marked (III), considerable (IV) and maximum (V) indices of surgical risk. The suggested classification is simple and objection enough. By means of the latter it is possible to choose beforehand the extent and character of the intervention, to delineate its provision, to obtain some comparative data on the advantages and shortcomings of different methods of anesthesia, reanimation, rehabilitation of patients and final results of treatment in surgical patients.