Sustained nitric oxide production via l-arginine administration ameliorates effects of intestinal ischemia-reperfusion.

BACKGROUND: The role of nitric oxide in intestinal ischemia-reperfusion is unclear-some studies link it to the harmful effects of ischemia-reperfusion, while others report it to be protective. We propose that nitric oxide levels diminish in the reperfusion period in conjunction with the onset of increased capillary permeability. The aim of this study is to determine the effect of supplementing nitric oxide synthase with its substrate, l-arginine, on development of local mucosal injury and systemic capillary leak. MATERIALS AND METHODS: Rats underwent 30 min of superior mesenteric artery occlusion followed by 4 h of reperfusion. The vehicle groups received l-arginine either intravenously (4 mg/kg/min) or into the intestinal lumen. The intravenous groups received l-arginine either before the ischemic event or after 30 min of reperfusion. Capillary leak in the gut and lung were measured, as were degree of mucosal injury and number of infiltrating neutrophils. Appropriate controls were performed. RESULTS: Thirty minutes of mesenteric ischemia followed by 4 h of reperfusion significantly increased gut and lung leak, neutrophil infiltration, and the severity of mucosal injury. l-Arginine given iv prior to ischemia inhibited lung leak, mucosal injury, and neutrophil infiltration. When arginine was given during the reperfusion period, lung leak and neutrophil infiltration but not mucosal injury were reduced. Intraluminal l-arginine reduced mucosa injury, but had no effect on capillary leak. CONCLUSIONS: Supplementation with l-arginine enhances NO production, resulting in reduced systemic endothelial dysfunction. This may act as a useful clinical adjunct in the management of trauma patients in preventing the development of ARDS and multiple organ failure.

Neutrophil priming state predicts capillary leak after gut ischemia in rats.

BACKGROUND: Multiple organ failure after serious injury or illness is a major determinant of mortality. An initial insult is believed to "prime" circulating neutrophils and induce systemic inflammation. Thereafter, a second insult will precipitate distant organ injury. The aim of these studies was to evaluate circulating neutrophil function after mesenteric ischemia-reperfusion to determine the neutrophil "priming state," a quantitative and clinically useful predictor of multiple organ failure. MATERIALS AND METHODS: Anesthetized Sprague-Dawley rats underwent superior mesenteric artery occlusion for 30 min or sham operation and were euthanized after 2, 6, or 24 h of reperfusion. Control animals had blood taken without any intervention. To determine changes in lung capillary permeability, another group of rats received Evan's blue, a dye that binds albumin, 1 h before sacrifice. Flow cytometric analysis was performed on 5 million white blood cells after removal of red cells by lysis and centrifugation. Neutrophil number, oxidative burst, and CD18 expression were measured. RESULTS: The number of circulating neutrophils was elevated similarly in rats subjected to sham operation or ischemia-reperfusion. Oxidative burst potential was increased at 2 h, maximum at 6 h, and normal at 24 h after reperfusion, but not in sham rats. CD18 expression was similar in all groups. There was a significant temporal correlation between the "priming state" of the circulating neutrophil, defined as the product of the neutrophil number times oxidative burst, and lung leak. CONCLUSIONS: The neutrophil "priming state" may allow the clinician to better predict those patients at greatest risk for multiple organ failure.

"Hear a just cause, O Lord, attend to my cry": organ donation and pastoral care.

Reports the findings of a survey of hospital chaplains, congregational clergy, and seminary students on the subject of organ donation. Points out the need for additional education and theological discussion focused on the donation of organs.

Self-reported hearing difficulty and audiometric thresholds in nursing home residents.

BACKGROUND: Nursing home practitioners usually assess the general health and functional abilities of each resident at the time of admission. If audiometric screening is not routinely available, assessment of the resident's hearing status will probably consist only of asking questions about hearing difficulty. In this study we explored which questions, when answered positively, were most strongly associated with moderate or severe hearing impairment. METHODS: A total of 198 newly admitted nursing home residents answered questions regarding their hearing in common listening situations, and then underwent audiometric assessment. RESULTS: Fifty-four percent of the residents had a pure tone average hearing level of > 25 dB at 500, 1000, and 2000 Hz and were therefore considered impaired. A single general question regarding hearing had a sensitivity of 69% in determining the presence of such impairment. Three specific questions which assessed hearing--in a group, while watching television, and while on the telephone--had a collective sensitivity of 83%. Asking the three questions was significantly (P = .003) more effective than asking only the general question. CONCLUSIONS: A set of specific questions significantly improved the identification of residents whose hearing loss affected their daily living activities compared with the use of a single hearing loss question.

The young adult with recent vision loss: a pilot case study.

When in the full course of a sighted life an individual becomes blind, it is an end of that sighted life. However it comes, it is the end to a way of life that had become part of that person. It is the end of familiar methods of doing things. Carroll (1961) believes that it is naive, if not superficial, to think of blindness as a blow to the eyes only, to sight only. He believes that it is a destructive blow to the self-image which one has carefully, though unconsciously, constructed throughout the lifetime.

Hearing loss in the nursing home. An institutional issue.

One hundred ninety-eight nursing home admissions underwent audiometric assessment and answered questions regarding hearing difficulty in common listening situations. Twenty-four percent demonstrated normal thresholds (0-25 dB) in the speech frequencies with only mild losses at 4000 Hz. Fifty-four percent had normal thresholds through the speech frequencies with moderate to profound losses at higher frequencies, or mild losses (26-40 dB) in the speech frequencies. Such persons would be at risk in daily listening situations. Twenty-two percent had moderate or greater losses (greater than 40 dB) through the entire frequency range. Sixteen percent of this significantly impaired group were not identified as having a hearing loss by either the admitting RN or physician. Sixty percent of residents reported trouble in a group if they could not see the speaker's face, when watching television, and/or when using the telephone. The data indicate that a systematic hearing screening program is the most reliable means of identifying hearing loss and functional hearing handicap on an individual basis. The high prevalence of hearing problems suggests that this handicap also needs to be addressed from the perspective of institutional policy. Staff training and environmental modification should be undertaken to give hearing support to the entire nursing home population.