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1.
Endocr Regul ; 47(2): 93-9, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23641790

ABSTRACT

OBJECTIVE: The ependymal cells, considered today as an active participant in neuroendocrine functions, were investigated by electron microscopy in the central canal of the lowest spinal cord, the filum terminale (FT), in adult rats. In this area of the spinal cord, the central canal is covered by a heterogeneous population of ependymal cells. The aim of the present work was to compare the regional features of the ependymal cells in two different parts of the FT with a special regard to their ultrastructure. METHODS: Two parts of the FT were selected for the ultrastructural observations: the rostral (rFT) and the caudal (cFT) ones. The rTF was removed at the level of the immediate continuation of the conus medullaris, while the cFT 30 mm further caudally. After formaldehyde fixation, the spinal cord was removed and cut into small blocks for electron microscopic processing. The material was embedded into durcupan, contrasted with uranyl acetate, lead citrate as well as osmium tetroxide, and investigated under JEOL 1200 EX electron microscope. RESULTS: In the rFT, the ependymal lining is pseudostratified and one-layered in the cFT, whereas the shape of the ependymal cells may vary from cuboidal to flatten in the rostro-caudal direction. The basal membrane of many ependymal cells possesses deep invaginations, so called "filum terminale labyrinths". Many neuronal processes occur in the pericanalicular neuropil. In contrast to the rFT, the cFT is less rich in the neuropil particles. Some of the ependymal cells concurrently reach both the intracanalicular and extracanalicular cerebrospinal fluid (CSF), thus they may represent a new variant of the ependymal cells designated as "bridge cells of the FT". CONCLUSIONS: The present data indicate that the FT ependymal cells exhibit clear differences in anatomy as well as ultrastructure that may reflect their distinct functional activity. Therefore, observations presented here may serve for the better understanding of the physiological role of the individual ependymal areas in this special portion of the rat spinal cord.


Subject(s)
Cauda Equina/cytology , Ependyma/cytology , Neurosecretory Systems/cytology , Spinal Cord/cytology , Animals , Axons/ultrastructure , Cauda Equina/ultrastructure , Dendrites/ultrastructure , Ependyma/ultrastructure , Male , Microscopy, Electron , Mitochondria/ultrastructure , Neuroglia/ultrastructure , Neuropil/cytology , Neuropil/ultrastructure , Neurosecretory Systems/ultrastructure , Rats , Rats, Sprague-Dawley , Spinal Cord/ultrastructure
2.
Neuroscience ; 159(2): 862-82, 2009 Mar 17.
Article in English | MEDLINE | ID: mdl-19174183

ABSTRACT

The effect of experimentally induced acute renal failure (ARF) on neuronal cell activation was investigated by immunohistochemistry for Fos and Fra-2 in the rat brain. ARF in rats was induced by bilateral nephrectomy (BNX), bilateral ureter ligature (BUL) and uranyl acetate injection with proper controls (sham-operation or saline injections, respectively). To follow the effect of the development of ARF, rats were killed 30 and 60 min, and 3, 12, 24 and 72 h after surgery, or 3 h to 12 days after uranyl acetate injections. In the BUL and BNX rats, urea and creatinine rose markedly in the plasma within 72 h, while in the uranyl acetate-injected rats the highest levels were observed on the 7th day, followed by a marked decline. At each time-point of the three different, experimentally induced ARF, the presence of Fos- and/or Fra-2-immunoreactive neurons was determined in 120 different brain areas and nuclei. In general, the 73 of 120 brain areas that showed time and intensity dependent activation in response to ARF can be classified into four groups: 1) biogenic amine (noradrenaline, adrenaline, histamine and 5-HT) expressing cell groups in the lower brainstem, 2) "stress-sensitive" forebrain areas, with regard to certain hypothalamic, limbic and cortical areas, 3) neuronal cell groups that participate in the central regulation of body and brain water and electrolyte homeostasis, including the circumventricular organs, and 4) central autonomic cell groups, especially visceral sensory cell groups in the brain, which are in primary, secondary or tertiary connections with renal afferents. Data presented here indicate that a wide variety of neurons in several regulatory mechanisms is affected by ARF-induced peripheral and central alterations.


Subject(s)
Acute Kidney Injury/classification , Acute Kidney Injury/pathology , Brain/metabolism , Brain/pathology , Neurons/metabolism , Acute Kidney Injury/etiology , Animals , Biogenic Amines/metabolism , Body Weight/drug effects , Body Weight/physiology , Creatine/blood , Disease Models, Animal , Fos-Related Antigen-2/metabolism , Ligation/adverse effects , Male , Nephrectomy/adverse effects , Oncogene Proteins v-fos/metabolism , Organometallic Compounds/toxicity , Rats , Rats, Wistar , Time Factors , Urea/blood
3.
Neuroscience ; 113(3): 653-62, 2002.
Article in English | MEDLINE | ID: mdl-12150785

ABSTRACT

The peptide apelin originating from a larger precursor preproapelin molecule has been recently isolated and identified as the endogenous ligand of the human orphan G protein-coupled receptor, APJ (putative receptor protein related to the angiotensin receptor AT(1)). We have shown recently that apelin and apelin receptor mRNA are expressed in brain and that the centrally injected apelin fragment K17F (Lys(1)-Phe-Arg-Arg-Gln-Arg-Pro-Arg-Leu-Ser-His-Lys-Gly-Pro-Met-Pro-Phe(17)) decreased vasopressin release and altered drinking behavior. Using a specific polyclonal antiserum against K17F for immunohistochemistry, the aim of the present study was to establish the precise topographical distribution of apelin immunoreactivity in colchicine-treated adult rat brain. Immunoreactivity was essentially detected in neuronal cell bodies and fibers throughout the entire neuroaxis in different densities. Cells bodies have been visualized in the preoptic region, the hypothalamic supraoptic and paraventricular nuclei and in the highest density, in the arcuate nucleus. Apelin immunoreactive cell bodies were also seen in the pons and the medulla oblongata. Apelin nerve fibers appear more widely distributed than neuronal apelin cell bodies. The hypothalamus represented, by far, the major site of apelin-positive nerve fibers which were found in the suprachiasmatic, periventricular, dorsomedial, ventromedial nuclei and in the retrochiasmatic area, with the highest density in the internal layer of the median eminence. Fibers were also found innervating other circumventricular organs such as the vascular organ of the lamina terminalis, the subfornical and the subcommissural organs and the area postrema. Apelin was also detected in the septum and the amygdala and in high density in the paraventricular thalamic nucleus, the periaqueductal central gray matter and dorsal raphe nucleus, the parabrachial and Barrington nuclei in the pons and in the nucleus of the solitary tract, lateral reticular, prepositus hypoglossal and spinal trigeminal nuclei. The topographical distribution of apelinergic neurons in the brain suggests multiple roles for apelin especially in the central control of ingestive behaviors, pituitary hormone release and circadian rhythms.


Subject(s)
Brain/cytology , Carrier Proteins/analysis , Neurons/chemistry , Animals , Apelin , Brain Chemistry , Carrier Proteins/biosynthesis , Carrier Proteins/immunology , Immunohistochemistry , Intercellular Signaling Peptides and Proteins , Male , Neurons/metabolism , Rats , Rats, Inbred WKY
4.
Neuroscience ; 108(1): 27-49, 2001.
Article in English | MEDLINE | ID: mdl-11738129

ABSTRACT

In brain, signaling pathways initiated by atrial natriuretic peptide, or transmitters which stimulate nitric oxide synthesis, increase cGMP as their second messenger. One important class of target molecules for cGMP is cGMP-dependent protein kinases, and in the present study, biochemical and immunocytochemical analyses demonstrate the widespread distribution of type II cGMP-dependent protein kinase in rat brain, from the cerebral cortex to the brainstem and cerebellum. Also, colocalization of cGMP-dependent protein kinase type II with its activator, cGMP, was found in several brain regions examined after in vitro stimulation of brain slices with sodium nitroprusside. In western blots, cGMP-dependent protein kinase type II was observed in all brain regions examined, although cerebellar cortex and pituitary contained comparatively less of the kinase. Immunocytochemistry revealed cGMP-dependent protein kinase type II in certain neurons, and occasionally in putative oligodendrocytes and astrocytes, however, its most striking and predominant localization was in neuropil. Electron microscopy examination of neuropil in the medial habenula showed localization of the kinase in both axon terminals and dendrites. As a membrane-associated protein, cGMP-dependent protein kinase type II often appeared to be transported to cell processes to a greater extent than being retained in the cell body. Thus, immunocytochemical labeling of cGMP-dependent protein kinase type II often did not coincide with the localization of kinase mRNA previously observed by others using in situ hybridization. We conclude that in contrast to cGMP-dependent protein kinase type I, which has a very restricted localization to cerebellar Purkinje cells and a few other sites, cGMP-dependent protein kinase type II is a very ubiquitous brain protein kinase and thus a more likely candidate for relaying myriad cGMP effects in brain requiring protein phosphorylation.


Subject(s)
Brain/enzymology , Isoenzymes/metabolism , Animals , Blotting, Western , Brain/metabolism , Cyclic GMP/metabolism , Cyclic GMP-Dependent Protein Kinases/metabolism , Immunohistochemistry , Male , Nitric Oxide/physiology , Rats , Rats, Inbred WKY , Sensitivity and Specificity , Staining and Labeling , Tissue Distribution
5.
J Neurochem ; 77(4): 1085-96, 2001 May.
Article in English | MEDLINE | ID: mdl-11359874

ABSTRACT

Apelin, a peptide recently isolated from bovine stomach tissue extracts, has been identified as the endogenous ligand of the human orphan APJ receptor. We established a stable Chinese hamster ovary (CHO) cell line expressing a gene encoding the rat apelin receptor fused to the enhanced green fluorescent protein, to investigate internalization and the pharmacological profile of the apelin receptor. Stimulation of this receptor by the apelin fragments K17F (Lys1-Phe-Arg-Arg-Gln-Arg-Pro-Arg-Leu-Ser-His-Lys-Gly-Pro-Met-Pro-Phe17) and pE13F (pGlu5-Arg-Pro-Arg-Leu-Ser-His-Lys-Gly-Pro-Met-Pro-Phe17) resulted in a dose-dependent inhibition of forskolin-induced cAMP production and promoted its internalization. In contrast, the apelin fragments R10F (Arg8-Leu-Ser-His-Lys-Gly-Pro-Met-Pro-Phe17) and G5F (Gly13-Pro-Met-Pro-Phe17) were inactive. The physiological role of apelin and its receptor was then investigated by showing for the first time in rodent brain: (i) detection of apelin neurons in the supraoptic and paraventricular nuclei by immunohistochemistry with a specific polyclonal anti-apelin K17F antibody; (ii) detection of apelin receptor mRNA in supraoptic vasopressinergic neurons by in situ hybridization and immunohistochemistry; and (iii) a decrease in vasopressin release following intracerebroventricular injection of K17F, or pE13F, but not R10F. Thus, apelin locally synthesized in the supraoptic nucleus could exert a direct inhibitory action on vasopressinergic neuron activity via the apelin receptors synthesized in these cells. Furthermore, central injection of pE13F significantly decreased water intake in dehydrated normotensive rats but did not affect blood pressure. Together, these results suggest that neuronal apelin plays an important role in the central control of body fluid homeostasis.


Subject(s)
Brain/physiology , Carrier Proteins/physiology , Receptors, Dopamine D2/physiology , Receptors, G-Protein-Coupled , Adipokines , Amino Acid Sequence , Animals , Apelin , Apelin Receptors , Blood Pressure/drug effects , CHO Cells , Carrier Proteins/chemistry , Carrier Proteins/pharmacology , Cerebral Ventricles/drug effects , Cerebral Ventricles/physiology , Colforsin/pharmacology , Cricetinae , Cyclic AMP/metabolism , Drinking Behavior/drug effects , In Situ Hybridization , Injections, Intravenous , Injections, Intraventricular , Intercellular Signaling Peptides and Proteins , Male , Mice , Molecular Sequence Data , Neurons/physiology , Peptide Fragments/administration & dosage , Peptide Fragments/pharmacology , RNA, Messenger/analysis , Rats , Rats, Inbred WKY , Receptors, Dopamine D2/drug effects , Receptors, Dopamine D2/genetics , Transfection , Vasopressins/blood , Water Deprivation
6.
J Comp Neurol ; 414(2): 255-66, 1999 Nov 15.
Article in English | MEDLINE | ID: mdl-10516595

ABSTRACT

Decussations of descending fibers of the hypothalamic paraventricular nucleus (PVN) were investigated by using Phaseolus vulgaris-leucoagglutinin (PHA-L) in intact and brainstem-operated rats. Fibers descend ipsilaterally along the brainstem and spinal cord and decussate at four levels: 1) Supramamillary decussations (SM). PVN fibers reach this area through the lateral hypothalamus and along the third ventricle in the dorsal hypothalamus. In the posterior hypothalamus some fibers crossover in the SM and terminate in the supramamillary region bilaterally. 2) Pontine tegmentum. PVN fibers run in the lateral part of the tegmentum arching to the basis of the pons. Some fibers crossover under the fourth ventricle. The locus ceruleus and the Barrington's nucleus receive bilateral innervation with ipsilateral dominance. 3) Commissural part of the nucleus of the solitary tract (NTS). The major crossover of PVN fibers is found here. The decussated fibers form a dense network here, and loop rostralward to innervate the entire NTS. A midsagittal knife-cut through the NTS eliminated paraventricular-fibers on the contralateral side. Synaptic contacts between PHA-L-labeled boutons and tyrozine hydroxilase-positive neurons were verified in the NTS. The caudal ventrolateral medulla also receives bilateral innervation. 4) Lamina X of the thoracic spinal cord. Paraventricular fibers enter the lateral funiculus ipsilaterally and innervate the intermediolateral cell column (IML). Some fibers cross the midline ventral and dorsal to the central canal running to the contralateral IML, at the level of the decussation. Our results demonstrated that paraventricular projections form a continuous descending pathway on their side of origin, and provide crossover fibers which may terminate segmentally without forming long tracts after crossover.


Subject(s)
Autonomic Nervous System/cytology , Paraventricular Hypothalamic Nucleus/cytology , Rats, Wistar/anatomy & histology , Solitary Nucleus/cytology , Spinal Cord/cytology , Animals , Catecholamines/analysis , Efferent Pathways , Hypothalamic Area, Lateral/cytology , Male , Microinjections , Microscopy, Electron , Nerve Fibers/chemistry , Nerve Fibers/ultrastructure , Phytohemagglutinins , Pons/cytology , Rats , Tegmentum Mesencephali/cytology
7.
Clin Exp Pharmacol Physiol Suppl ; 26: S14-22, 1999 Apr.
Article in English | MEDLINE | ID: mdl-10386249

ABSTRACT

1. Dopamine (DA) is a protective agent in the gastrointestinal (GI) tract in both rats and humans. Therefore, we have studied the site of DA production in rat and human GI tract using a variety of techniques, including immunocytochemistry (ICC), in situ hybridization histochemistry, reverse transcription-polymerase chain reaction, HPLC, western blotting and immunoelectron microscopy. 2. We found very high concentrations of DA that persisted after chemical sympathectomy (CS) in the gastric juice, the stomach mucosa and in the pancreas. Both the stomach mucosa and the pancreas also had tyrosine hydroxylase (TH) activity, most of which remained after CS. Double-labelling ICC showed that acid-producing parietal cells and the exocrine pancreas must also be capable of producing DA. 3. We isolated rat stomach parietal cells by cell fractionation and found that both DA and TH activity are present in isolated (denervated) parietal cells. These cells also have other features of aminergic cells: they are immuno- (and mRNA) positive for the DA plasma membrane transporter and vesicular monoamine transporter(s). In both gastric and duodenal mucosa, we demonstrated the presence of significant amounts of the D5 receptor that could serve as a target for locally produced DA. 4. Because DA, its biosynthetic enzymes and its transporters are also found in parietal cells in the human stomach, a mucosal protective system involving DA could be important clinically.


Subject(s)
Digestive System/metabolism , Dopamine/metabolism , Receptors, Dopamine D1/analysis , Animals , Digestive System/cytology , Digestive System/pathology , Dopamine/pharmacokinetics , Humans , Male , Perfusion , Rats , Rats, Sprague-Dawley , Receptors, Dopamine D5 , Tyrosine 3-Monooxygenase/metabolism
8.
Cell Tissue Res ; 293(2): 227-33, 1998 Aug.
Article in English | MEDLINE | ID: mdl-9662645

ABSTRACT

We mapped the distribution of calretinin-immunoreactive neuron populations in a circumventricular organ of the rat, the area postrema, and investigated their sensitivity to excitotoxic stimuli mediated by subcutaneously administered monosodium glutamate. We were specifically interested to ascertain whether the presence of calretinin can, per se, confer an in vivo intrinsic resistance for area postrema neurons to glutamate excitotoxicity. We found that dense populations of calretinin-positive neurons displayed a subregional compartmentation in coronal sections of the area postrema along its rostrocaudal axis. We demonstrated that calretinin-positive neurons differ in their sensitivities to monosodium glutamate depending on their position within the area postrema. Neurons in the caudal area postrema were the most sensitive ones, while those in the rostral area postrema were spared of degeneration. We conclude that calretinin-positive neurons in the area postrema are not uniformly protected against glutamate excitotoxicity. It is possible that differences in the local concentrations of monosodium glutamate due to regional heterogeneities in density and permeability of the capillary bed rather than neuronal expression of calretinin account for the observed effects.


Subject(s)
Neurons/drug effects , S100 Calcium Binding Protein G/metabolism , Sodium Glutamate/pharmacology , Animals , Calbindin 2 , Male , Nerve Degeneration , Neurons/metabolism , Rats , Rats, Wistar
9.
Neuroscience ; 85(1): 45-52, 1998 Jul.
Article in English | MEDLINE | ID: mdl-9607701

ABSTRACT

The distribution of vasoactive intestinal polypeptide-immunoreactive neuronal structures in the barrel cortex (posteromedial barrel subfield) of adult rats was analysed after unilateral removal of the vibrissal follicles of row C in neonatal rats. The hypothesis was tested whether the distribution of vasoactive intestinal polypeptide-immunoreactive structures depends on the normal anatomical organization of the specific sensory input. After three months survival the distribution of the vasoactive intestinal polypeptide-immunoreactive structures was morphometrically evaluated. This approach revealed alterations in the contralateral posteromedial barrel subfield, where the disappearance of barrel row C and a substantial increase in size mainly of barrel row D, but also of other rows could be detected. Increase in row D included both barrels and the interspace (septal segments between barrels in one row). As vasoactive intestinal polypeptide immunoreactivity of the barrel field was found previously to be localized in synaptic boutons involved in symmetric synapses, our present findings suggest that (i) the interspace is enriched in inhibitory vasoactive intestinal polypeptide-immunoreactive synapses as opposed to the excitatory thalamocortical input reaching the barrel hollow, (ii) the spatial distribution of the vasoactive intestinal polypeptide system in the barrel cortex is closely associated with the neuronal organization of the sensory input and reacts with a considerable plasticity to lesion-induced changes of the input, and (iii) the compensatory barrel hypertrophy in a row neighbouring the deafferented row involves an increasing number of vasoactive intestinal polypeptide-immunoreactive synapses per barrel.


Subject(s)
Animals, Newborn/physiology , Somatosensory Cortex/metabolism , Somatosensory Cortex/physiology , Vasoactive Intestinal Peptide/metabolism , Vibrissae/physiology , Afferent Pathways/physiology , Animals , Denervation , Immunohistochemistry , Neuronal Plasticity/physiology , Rats , Rats, Wistar , Somatosensory Cortex/pathology , Synapses/metabolism
11.
Neuroimmunomodulation ; 4(5-6): 250-7, 1997.
Article in English | MEDLINE | ID: mdl-9650818

ABSTRACT

Different neuropeptide-containing nerve fibers were investigated to clarify their role in the pathogenesis of Crohn's disease (CD) using immunohisto- and immunocytochemical techniques. Specimens were obtained from patients with CD from grossly affected colonic regions, from biopsies obtained from patients with CD treated with mesalazine and from control individuals. Quantitative analysis was made for the changes of the number of nerve terminals and their vesicle contents. The distribution pattern of all immunoreactive (IR) nerve fibers was similar both in the control and in the surgical specimens as well as in the biopsies obtained. The number of the synapses, the IR nerve fibers and their vesicle content were markedly decreased in the grossly affected colonic regions. Some degenerated axons were found in close proximity to the plasma cells. Immunocytochemistry demonstrated that substance P, vasoactive intestinal polypeptide and somatostatin IR nerve fibers were in direct contact with the plasma cells, lymphocytes and other immunocompetent cells. The gap between the membranes of immunoreactive nerve terminals and immunocompetent cells was 20-200 nm, in a few cases even less. In the mesalazine-treated group the number of the IR nerve terminals as well as their vesicle content was increased. These results suggest that changes in the number of different neuropeptide-containing nerve terminals and their content might alter the neuroimmunological processes, because these peptides are known to be immunoregulators.


Subject(s)
Autoimmune Diseases/pathology , Colon/innervation , Crohn Disease/pathology , Enteric Nervous System/physiopathology , Intestinal Mucosa/innervation , Neuroimmunomodulation , Adolescent , Adrenal Cortex Hormones/pharmacology , Adrenal Cortex Hormones/therapeutic use , Adult , Anti-Inflammatory Agents/pharmacology , Anti-Inflammatory Agents/therapeutic use , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Anti-Inflammatory Agents, Non-Steroidal/therapeutic use , Autoimmune Diseases/drug therapy , Autoimmune Diseases/immunology , Colon/drug effects , Colon/immunology , Crohn Disease/drug therapy , Crohn Disease/immunology , Enkephalin, Leucine/analysis , Enteric Nervous System/chemistry , Enteric Nervous System/drug effects , Enteric Nervous System/ultrastructure , Female , Humans , Immunosuppressive Agents/pharmacology , Immunosuppressive Agents/therapeutic use , Intestinal Mucosa/drug effects , Intestinal Mucosa/immunology , Male , Mesalamine/pharmacology , Mesalamine/therapeutic use , Middle Aged , Nerve Fibers/chemistry , Nerve Fibers/pathology , Neuroimmunomodulation/drug effects , Neuropeptide Y/analysis , Plasma Cells/ultrastructure , Somatostatin/analysis , Steroids , Substance P/analysis , Synapses/chemistry , Synapses/ultrastructure , Vasoactive Intestinal Peptide/analysis
12.
Brain Res ; 690(1): 141-4, 1995 Aug 28.
Article in English | MEDLINE | ID: mdl-7496801

ABSTRACT

By using double immunolabeling light and electron microscopic techniques, dense neuronal network of calcitonin-related peptide (CGRP) has been visualized in the nucleus of the solitary tract with complete overlapping of the tyrosine hydroxylase (TH)-containing cells. TH-immunoreactive perikarya and dendrites were seen in synaptic contact with CGRP-immunopositive fibers, indicating that CGRP, by carrying sensory signals may influence autonomic regulatory mechanisms in the NTS through local catecholaminergic neurons.


Subject(s)
Autonomic Nervous System/chemistry , Calcitonin Gene-Related Peptide/analysis , Norepinephrine/analysis , Solitary Nucleus/chemistry , Animals , Immunoenzyme Techniques , Male , Rats , Rats, Sprague-Dawley , Solitary Nucleus/cytology , Tyrosine 3-Monooxygenase/analysis
13.
Anat Embryol (Berl) ; 182(1): 69-78, 1990.
Article in English | MEDLINE | ID: mdl-2240594

ABSTRACT

Numerous cells were observed to show intense vasoactive intestinal polypeptide (VIP) immunoreactivity from birth to postnatal day 8 in the subventricular zone of the rat occipital hemisphere. This cell population was markedly reduced by postnatal day 8, but isolated clusters of VIP cells persisted into adulthood. In addition, long, L-shaped VIP fibers were seen in the hemispheric wall up to postnatal day 16, but not in the adult. Parallel to the reduction in number of the subventricular VIP cells an increasing number of VIP cells appeared in the neo- and allocortex, developing by postnatal day 12 all the features of the mature cortical bipolar fusiform neurons. As possible alternatives, the migration of subventricular VIP cells into the cortex, the transient character of the subventricular VIP population or the expression of VIP by radial glia are discussed.


Subject(s)
Occipital Lobe/cytology , Vasoactive Intestinal Peptide/biosynthesis , Aging , Animals , Female , Immunohistochemistry , Male , Neurons , Occipital Lobe/growth & development , Occipital Lobe/metabolism , Rats , Rats, Inbred Strains
14.
Anat Embryol (Berl) ; 178(3): 197-206, 1988.
Article in English | MEDLINE | ID: mdl-3414974

ABSTRACT

Vasoactive intestinal polypeptide (VIP)-immunoreactive cells in the primary visual cortex of the rat were classified on the basis of ramification pattern of cell processes. The distribution of cells over cortical layers, and proportions of cell classes relative to total cell numbers were evaluated by means of quantitative methods. Two main types of VIP-positive neurons, the bipolar and the multipolar were distinguished constituting 76% and 24% of the VIP populations, respectively. The axons of vertically oriented bipolars were observed to ramify within a column around the descending dendrite. By contrast, multipolar cells have a non-oriented ramification pattern. The two overlapping axonal systems form the VIP-innervation of the rat visual cortex.


Subject(s)
Vasoactive Intestinal Peptide/analysis , Visual Cortex/cytology , Animals , Dendrites/analysis , Dendrites/ultrastructure , Female , Immunohistochemistry , Male , Neurons/analysis , Neurons/ultrastructure , Rats , Visual Cortex/analysis
15.
Brain Res ; 433(1): 131-8, 1987 Nov.
Article in English | MEDLINE | ID: mdl-3676849

ABSTRACT

Olfactory bulbs of 8-, 12-, 16- and 30-day-old rats were studied by means of immunocytochemistry using antibodies to glial fibrillary acidic protein (GFAP) and with the Toluidine blue-staining of semithin sections. Until day 12 the GFAP-reaction revealed a radial glia system, the fibres of which extended from the axial ventricular cleft to the surface. From day 16 onwards radial fibres were gradually replaced by typical astrocytes. The lack of proliferative activity within the bulb during the early postnatal period suggests that its cells are generated at and migrating from an external site. An intensely proliferating area was detected in the frontal lobe subventricular layer from where a bundle of migratory cells extends into the bulb. Radial glia may thus be of importance in guiding the migration of cells from this axial bundle to more peripheral regions of the olfactory bulb.


Subject(s)
Glial Fibrillary Acidic Protein/analysis , Neuroglia/cytology , Olfactory Bulb/growth & development , Animals , Cell Division , Cell Movement , Female , Male , Neuroglia/analysis , Olfactory Bulb/anatomy & histology , Olfactory Bulb/immunology , Rats
16.
Acta Morphol Hung ; 34(1-2): 59-71, 1986.
Article in English | MEDLINE | ID: mdl-2882648

ABSTRACT

Somatostatin-containing nerve elements were identified by light and electron microscopic immunocytochemistry in the rat small intestine. Labelled nerve cell bodies were found in the submucous plexus. In the myenteric plexus and the mucosa, numerous somatostatin positive fibers were found. Labelled fibers were observed in the vicinity of blood vessels, close to the smooth muscle cell membrane and to the lamina basalis of epithelial cells. In both plexuses, there were many synapses between the labelled and unlabelled nerve elements. After transection of the nn. mesenteric some of the degenerated nerve fibers originating outside the wall of the small intestine contained also somatostatin immunoreactivity. These observations suggest that somatostatin nerves not only influence the blood flow, but participate in regulation of smooth muscle activity and, by the aid of their interneuronal synapses they have a regulatory or transmitter effect on the other intrinsic nerve elements.


Subject(s)
Intestine, Small/innervation , Nerve Fibers/analysis , Somatostatin/analysis , Animals , Female , Immunoenzyme Techniques , Intestinal Mucosa/innervation , Intestinal Mucosa/ultrastructure , Male , Microscopy, Electron , Nerve Fibers/ultrastructure , Rats , Rats, Inbred Strains
17.
Histochemistry ; 84(3): 275-8, 1986.
Article in English | MEDLINE | ID: mdl-3710833

ABSTRACT

Vibratome sections of the cerebral cortex, hippocampus and cerebellum were immunostained for alpha-tubulin using the TU-Ol monoclonal antibody. In all three regions, electron microscopy of the immunostained preparations revealed--in addition to the previously described reaction of pyramidal apical dendritic microtubules--consistent staining of the ribosomal apparatus of astrocytes.


Subject(s)
Astrocytes/analysis , Tubulin/analysis , Animals , Astrocytes/ultrastructure , Cerebellum/analysis , Cerebral Cortex/analysis , Hippocampus/analysis , Microscopy, Electron , Rats , Ribosomes/analysis , Tubulin/immunology
18.
Neuroendocrinology ; 42(6): 522-5, 1986.
Article in English | MEDLINE | ID: mdl-3703166

ABSTRACT

The source and location of serotonin in the intermediate lobe of the rat pituitary were determined by high performance liquid chromatography and immunohistochemistry. Serotonin is present in the intermediate lobe in nerve fibers and terminals, in mast cells and in elements in the blood circulating in vessels on the surface of the lobe.


Subject(s)
Pituitary Gland, Posterior/metabolism , Serotonin/metabolism , Animals , Chromatography, High Pressure Liquid , Dopamine/analysis , Female , Mast Cells/metabolism , Pituitary Gland, Posterior/analysis , Pituitary Gland, Posterior/blood supply , Rats , Rats, Inbred Strains , Serotonin/blood
19.
Histochemistry ; 82(5): 491-6, 1985.
Article in English | MEDLINE | ID: mdl-4030403

ABSTRACT

Rat cortical and hippocampal pyramidal cells were immunocytochemically investigated using the TU-01 monoclonal antibody recognizing alpha-tubulin. The isotypic specificity of this antibody is distinct from that of other available alpha-tubulin antibodies; therefore, an intracellular heterogeneity among neuronal microtubules could be revealed by observing intensely immunostained apical dendritic microtubules in the complete absence of staining of the microtubules in the basal dendrites and perikarya of the same pyramidal cells.


Subject(s)
Cerebral Cortex/metabolism , Hippocampus/metabolism , Tubulin/classification , Animals , Cerebral Cortex/cytology , Cerebral Cortex/ultrastructure , Female , Hippocampus/cytology , Hippocampus/ultrastructure , Histocytochemistry , Immunochemistry , Male , Microscopy, Electron , Neurons/metabolism , Neurons/ultrastructure , Rats , Tissue Distribution , Tubulin/metabolism
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