In vitro digestion and microbial fermentation of dried food residues, a potential "new" component for pet food, and different non-digestible carbohydrate sources.

Food residues are often fed to dogs in private households and might also be a potential "new" ingredient for pet food in the future. As food residues might contain not only digestible, but also fermentable substrates, an effect on the intestinal microbiota can be assumed. In the present study, two batches of dried food residues (DFR) collected from hotels in Crete were microbially fermented in an in vitro batch culture system with canine fecal inoculum: non-sterile DFR including meat (DFRm), sterile DFR including meat (DFRms) and sterile DFR without meat (DFRwms). Different non-digestible carbohydrate sources (beet pulp, wheat bran, inulin, carrot pomace, brewer´s spent grains, cellulose and lignocellulose) were included for comparison. Inulin, cellulose and lignocellulose were only used as raw materials, while the other test substrates were incubated as raw and enzymatically pre-digested substrates. After incubation for 24 hours, the raw food residues markedly increased the concentrations of bacterial metabolites in the fermenters, although smaller effects were observed for the DFRwms. When the enzymatically pre-digested food residues were incubated, the effects were more pronounced for the DFRms and DFRwms. In general, when compared with the other test substrates, the food residues were microbially fermented to a comparable or partly higher extent. Interestingly, high n-butyrate concentrations were measured in the inocula, both after incubation of the raw and pre-digested food residues. In conclusion, the food residues contained enzymatically digestible and microbially fermentable substrates. If considered as a potential future ingredient for pet food, a standardization of the collection and processing of food residues might be necessary in order to reduce compositional variability and varying effects on the intestinal microbiota.

Impact of the dietary inclusion of dried food residues on the apparent nutrient digestibility and the intestinal microbiota of dogs.

The use of food residues for animal nutrition might imply ecological and economic advantages; however, their effects as a potential ingredient have not yet been evaluated in dogs. In the present study, four diets with 0, 5, 10 and 15% dried food residues (DFR), derived from hotel catering, were fed to 10 healthy adult dogs. At the end of each three-week feeding period, faeces and blood were collected. The apparent nutrient digestibility was calculated by the dietary inclusion of titanium dioxide as an inert marker. The results demonstrated that the apparent crude protein digestibility and ether extract digestibility decreased with increasing amounts of DFR in the diets (p < 0.05). In addition, an increase of the faecal concentrations of acetic acid, propionic acid, n-butyric acid and total short-chain fatty acids (SCFA) was observed (p < 0.05). Faecal ammonium and lactate concentrations, as well as plasma phenol and indole concentrations, were not linearly affected by the dietary inclusion of DFR. The relative abundance of Fusobacteria in the faeces of the dogs decreased, and the relative abundance of Actinobacteria and Bacteroidetes increased with increasing amounts of DFR in the diets (p < 0.05). In conclusion, the DFR seemed to be intensively fermented by the intestinal microbiota of the dogs, as indicated by the increased faecal SCFA concentrations and the shifts in the composition of the faecal microbiota. Dietary inclusion levels of up to 5% can be recommended based on our results, as the observed lower apparent crude protein and ether extract digestibility might limit the use of food residues for dogs at higher amounts.

Bioactive Compounds in Food Waste: A Review on the Transformation of Food Waste to Animal Feed.

Bioactive compounds are substances which are present in foods in small amounts and have the ability to provide health benefits. Bioactive compounds include but are not limited to long-chain polyunsaturated fatty acids, vitamins, carotenoids, peptides, and polyphenols. The aim of the present study is to review literature for potential bioactive compounds present in food waste material and discuss the transformation of food waste to animal feed under the perspective that usage of food waste, rather than disposal, may tackle food insecurity and provide health benefits. Finally, applications in poultry and swine nutrition, with emphasis on the presence of fatty acids on food waste material, are discussed.