Phytoremediation: A green and low-cost technology to remediate herbicides in the environment.

Pesticide dependence is one of the main disadvantages of agriculture. Despite the advances in biological control and integrated management of plant pests and diseases in recent years, herbicides are still essential for weed control and constitute the main class of pesticides worldwide. Herbicide residues in water, soil, air, and non-target organisms are among the biggest agricultural and environmental sustainability obstacles. Therefore, we suggest an environmentally viable alternative to reduce the harmful effects of herbicide residues, a technology called phytoremediation. Remediating plants were grouped into herbaceous, arboreal, and aquatic macrophytes. Phytoremediation can reduce the loss of at least 50% of all herbicide residues to the environment. Among the herbaceous species reported as phytoremediators of herbicides, the Fabaceae family was mentioned in more than 50% of reports. This family is also among the main species of trees reported. Regarding the most reported groups of herbicides, it is observed that most of them, regardless of the group of plants, are triazines. Processes such as extraction or accumulation are the best known and reported for most herbicides. The phytoremediation may be effective against chronic or unknown herbicide toxicity. This tool can be included in proposals for management plans and specific legislation in countries, guaranteeing public policies to maintain environmental quality.

Neuroprotective potential of Cannabis sativa-based oils in Caenorhabditis elegans.

Substances from the Cannabis sativa species, especially cannabidiol (CBD) and Delta-9-tetrahydrocannabinol (Δ9-THC), have attracted medical attention in recent years. The actions of these two main cannabinoids modulate the cholinergic nervous system (CholNS) involving development, synaptic plasticity, and response to endogenous and environmental damage, as a characteristic of many neurodegenerative diseases. The dynamics of these diseases are mediated by specific neurotransmitters, such as the GABAergic nervous system (GNS) and the CholNS. The nematode Caenorhabditis elegans is an important experimental model, which has different neurotransmitter systems that coordinate its behavior and has a transgene strain that encodes the human ß-amyloid 1-42 peptide in body wall muscle, one of the main proteins involved in Alzheimer´s disease. Therefore, the objective of this study was to evaluate the protective potential of terpenoids found in C. sativa in the GNS and CholNS of C. elegans. The effect of two C. sativa oils with variations in CBD and THC concentrations on acetylcholinesterase (AChE) activity, lipid peroxidation, and behavior of C. elegans was evaluated. C. sativa oils were efficient in increasing pharyngeal pumping rate and reducing defecation cycle, AChE activity, and ROS levels in N2 strains. In the muscle:Abeta1-42 strain, mainly when using CBD oil, worm movement, body bends, and pharyngeal pumping were increased, with a reduced AChE activity. Consequently, greater investments in scientific research are needed, in addition to breaking the taboo on the use of the C. sativa plant as an alternative for medicinal use, especially in neurodegenerative diseases, which have already shown positive initial results.

Redox status upon herbicides application in the control of Lolium multiflorum (2n and 4n) as weed.

Lolium multiflorum Lam. is a winter weed of difficult control found as diploid (2n) and tetraploid plants (4n). Our study aimed to evaluate the responses of antioxidant enzymes and lipid peroxidation, in both diploid and tetraploid ryegrass varieties. Treatments consisted of control plants (without any herbicide application), and four herbicides with different mechanisms of action. Leaf material was collected 36 h after treatment imposition to determine the lipid peroxidation by ferrous oxidation-xylenol (FOX) content, and the activity of the enzymes superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), glutathione-S-transferase (GST), and δ-aminolevulinic acid dehydratase (ALAD). Both ryegrass varieties showed oxidative stress mainly due to a downregulated decreased (>31%) in SOD activity and an increase (>32%) in lipid peroxidation (FOX), mainly in ryegrass genotypes exposed to haloxyfop, glyphosate, and iodosulfuron. On the other hand, clethodim-treated plants had an increase in SOD and APX activities, associated with a reduced ALAD activity in both 2n (32%) and 4n (11%) genotypes. In general, the 2n genotype was more affected than the 4n genotype.

Does doses and time of 2, 4-D application interfere in the physiology and wheat grains yield components?

The identification of the application stage and correct dose of 2,4-dichlorophenoxyacetic acid (2,4-D) herbicide is important so that wheat is not harmed. In view of this, the objective of this study was to evaluate the effect of 2,4-D doses applied at different development stages of wheat crop. The experiment was conducted in a randomized block design, arranged in a 4 × 5 factorial scheme, with four replications. In factor A, the application stages (before tillering, tillering, first node and booting) were allocated and the doses of 2,4-D (0, 349, 698, 1047 and 1396 g.ha­1) were allocated in factor B. The variables evaluated were phytotoxicity at 7, 14, 21, 28 and 35 days after application of the treatments (DAT), photosynthetic activity, CO2 internal concentration, stomatal conductance, efficient water use and carboxylation efficiency. The number of spikes·m­2, spike length and number of full and sterile grains were determined in the preharvest. Thousand grain mass, grain yield and hectoliter weight were determined after harvest. The results demonstrate that the herbicide caused phytotoxicity to wheat, being greater in increasing doses and mainly before tillering, causing grain sterility and decreased productivity. The other yield components did not present difference when increasing the dose and application in different stages as well as the physiological variables. The increase of the 2,4-D doses applied before tillering and in the booting stage caused linear decrease in wheat grain yield.

Interaction between pesticides applied alone or in mixtures in corn.

The objective of this work was to evaluate the selectivity of herbicides applied alone or in tank mix, and also of combinations of different classes of pesticides (fungicides, herbicides and insecticides) on corn. An experiment was installed in the 2017/18 crop to assess herbicide selectivity, and repeated in 2018/19. The second experiment aimed to assess the selectivity of mixtures of different classes of pesticides, and was installed only in 2017/18. The experimental design adopted in the tests was randomized blocks, with four replicates. In each trial, a total of 16 treatments were evaluated, plus a control. At 7, 14 and 21 days after the application of treatments the phytotoxicity to corn was evaluated and the yield components and grain yield were determined. Herbicides caused low levels of injury to the corn plants, with small variations in yield components, and affected crop yield differently. The mixtures of pesticides promoted low phytotoxicity, affected yield components inconsistently and caused changes in corn yield. Although these herbicides and other pesticides are registered for use in corn crops, application alone or in tank mix of certain molecules can cause losses in grain yield.

Tank mixture of glyphosate and 2,4-D applied on RR® maize crop.

Herbicide tank-mixing is often carried out by farmers aiming to control weeds in crops. Thus, it is important to evaluate the effects of these mixtures regarding the selectivity of crops. The aim of this work is to assess the selectivity of increasing doses of herbicides (glyphosate and 2,4-D), applied alone or as a mixture, on maize crop. Two experiments were carried out in randomized blocks, with four replications, during two crop years. The evaluated variables were: phytotoxicity, plant height, stem diameter, ear length, number of rows per ear, number of grains per row, mass of 1,000 grains and grain yield. The herbicide, 2,4-D, when applied in mixture with glyphosate caused severe phytotoxicity symptoms on maize. Grain yield and growth characteristics were not affected by the herbicides applied alone or as a mixture. Increasing doses of 2,4-D caused severe phytotoxicity symptoms on hybrid Status VIP3. Increasing doses of glyphosate did not show phytotoxicity symptoms and are not directly correlated to any injury caused by 2,4-D in the SX 5885 VIP3 maize hybrid.

Morphophysiological characteristics of Brazilian bean genotypes related with sulfentrazone tolerance.

In the bean crop there are limited options of herbicides to control eudicotyledons weeds in pre-emergence and sulfentrazone presents potential to be used in a weed management program. The aim of this study was to determine the tolerance of a large number of Brazilian bean genotypes to sulfentrazone and their relation with morphophysiological characteristics, associating it to market classes, seed size and their center of origin. It was evaluated the effect of sulfentrazone application (400 g a.i. ha-1) in pre-emergence of 40 Brazilian bean genotypes, 36 of which were common-bean (Phaseolus vulgaris), two cowpeas (Vigna unguiculata), one adzuki bean (Vigna angularis) and one mung bean (Vigna radiata). There was high tolerance variation of the genotypes to sulfentrazone. Morphological parameters were strongly related with relative tolerance. Common bean genotypes that had medium to large seed size (cranberry and carioca) were more tolerant, while small-sized seed genotypes were more sensitive (black). Andean genotypes were more tolerant than Mesoamerican genotypes. Within the Mesoamerican group, medium-sized seed genotypes (carioca beans) were more tolerant than small-sized seed (black beans). Considering only the Vigna genera, cowpea was highly tolerant to sulfentrazone, while mung bean showed intermediary response and adzuki bean was completely sensible.

Mobility and environmental monitoring of pesticides in the atmosphere - a review.

Knowledge of the partition mechanisms in the agrochemical environment is fundamental for understanding their behavior within an ecosystem and mitigating possible adverse effects of these products. In this review, the objective was to present the main transport mechanisms, physical-chemical properties, and atmospheric monitoring methodologies of the most diverse types of agrochemicals used in agriculture that can reach the atmosphere and affect different compartments. It has been verified that volatilization is one of more considerable significance of the various forms of transport since a significant part of the applied pesticides can volatilize in a few days. As for monitoring these compounds in the atmosphere, both passive and active sampling have their advantages and disadvantages. Passive samplers allow sampling in large quantities and at remote locations, in addition to making continuous measurements, while active samplers have the advantage of being able to detect low concentrations and continuously. Since a significant portion of the applied pesticides is directed to the atmosphere, monitoring makes it possible to understand some properties of the pesticides present in the air. This monitoring can be done from different existing methodologies based on adopted criteria and existing technical standards. Graphical representation of mobility and environmental monitoring of atmospheric pollutants from pesticides.

Selectivity of metsulfuron applied to soybean before sowing in different intervals and soils.

This work aimed to evaluate the selectivity of the herbicide metsulfuron applied at different times on the development of soybeans grown in soils with different characteristics. The experiment was conducted in a randomized block design, in a factorial scheme (4 x 4), with four replicates. Factor A was application time (0, 15, 30, and 45 days before sowing, DBS) and factor B was soil type (Erechim, Itaqui, Piratini, and Santa Maria). Soybean plants cultivated in the Erechim soil showed moderate phytotoxicity, with greater damage to the leaf area and plant dry matter, mainly after application at 30 DBS. Those cultivated in Itaqui soil showed gradual phytotoxicity between 14 and 28 days after emergence (DAE). Soybean plants grown in the Piratini and Santa Maria soils showed the highest phytotoxicity and photosynthetic reduction, mainly at 15 and 0 DBS. Metsulfuron application at 45 DBS caused reduced plant growth by up to 40%, and reduced shoot development (30%) in soybean plants grown in Piratini and Santa Maria soils, respectively. There were gradual changes in phytotoxicity and the morphophysiological traits of soybean plants exposed to the residual effect of metsulfuron in different soils, which indicates that soybeans should be sown more than 45 days after the application of metsulfuron, regardless of soil characteristics.

Remedial capacity of diclosulam by cover plants in different edaphoclimatic conditions.

Pre-emergent herbicides have been developed for their long residual effect; however, they can make sensitive successor cultures unfeasible. Waste remediation techniques are needed for sustainable agricultural systems; for this, the edaphoclimatic asymmetries in the country must be considered. This study aimed to evaluate the remedial capacity of the herbicide diclosulam by covering crops under different edaphoclimatic conditions. Treatments were arranged in an n × 2 factorial scheme, the first factor being the number of plant species in each location and the second being the presence or absence of diclosulam in the soil. The physiological and growth characteristics of 15 potential phytoremediation species were evaluated. Herbicide residues in the environment were positively correlated with the soil pH; organic matter, aluminum, and silt contents; and aluminum saturation. The effectiveness of phytoremediation varied between species and between regions. Plant species suitable for efficient phytoremidation systems of diclosulam residues were Canavalia ensiformis for Couto Magalhães de Minas, Cajanus cajan and Canavalia ensiformis for Diamantina, Raphanus sativus for Erechim and Cajanus cajan for São João Evangelista.

Soil compaction and cover with black oat on soybean grain yield in lowland under no-tillage system / Compactação do solo e cobertura com aveia preta na produtividade de grãos de soja em várzea sob sistema de plantio direto

ABSTRACT: Brazil is the largest exporter and second largest producer of soybean grains. Most of this production is from plants grown under no-tillage system (NT). This research evaluated the effect of soil compaction, and different amounts of black oat residues on the soil surface on soybean growth and grain yield in lowland under NT. The experiment was conducted in a completely randomized design with seven treatments and four replications, in the 2016/2017 and 2017/2018 crop seasons. The treatments consisted of: 1) winter fallow without soil compaction (WF); 2) winter fallow with soil compaction (WF-C); 3) black oats and complete removal of surface residues, with soil compaction (0R-C); 4) black oats and removal half of surface residues, with soil compaction (0.5R-C); 5) black oats without surface residue removal, with soil compaction (1R-C); 6) black oats without surface residue removal, plus the residues from treatment 3, with soil compaction (2R-C); 7) black oats without surface residue removal, without soil compaction (1R). When the soybean plants were at the phenological stage R2, they were evaluated nodule, root and shoot dry matter, nitrogen contents, plant height, and grain yield. The soil physical properties were evaluated in the 0.0-0.05, 0.10-0.15 and 0.20-0.25 m layers. The soybean aerial dry matter is > 38% in non-compacted soil in year with soil water excess, regardless of the amount of surface oat straw. In year with small water deficit, soil with more surface oat straw produced > 5% shoot dry matter and > 4% of soybean grain, regardless of compaction. The plant growth and grain yield soybean in lowland varied according to the water conditions, and were affected by soil compaction and amounts of black oats residues on soil surface.

RESUMO: O Brasil se destaca na produção de soja, sendo o maior exportador e o segundo maior produtor do grão e a maior parte é cultivado em sistema plantio direto (SPD). Objetivou-se avaliar o efeito da compactação, cultivo e quantidades de palha de aveia preta na superfície do solo sobre o crescimento e o rendimento de grãos de soja em SPD em terras baixas. O delineamento experimental foi inteiramente casualizado com sete tratamentos e quatro repetições. Os tratamentos, nas safras 2016/17 e 2017/18, foram: 1) Pousio no inverno sem compactação (WF); 2) Pousio no inverno + compactação (WF-C); 3) Aveia preta e retirada da palha superficial + compactação (0R-C); 4) Aveia e retirada da metade da palha + compactação (0,5R-C); 5) Aveia + permanência da palhada + compactação (1R-C); 6) Aveia + a palha retirada do tratamento 3 (2 doses palha) + compactação (2R-C); 7) Aveia + permanência da palha, sem compactação (1R). No estádio fenológico R2 a soja foram avaliadas a massa seca de nódulos e de raízes, massa seca e nitrogênio da parte aérea, altura de plantas e produtividade de grãos. No solo foram avaliadas propriedades físicas nas camadas de 0,0-0,05; 0,10-0,15 e 0,20-0,25 m. A produção de massa seca da parte aérea de soja é > 38% em solo não compactado em ano com excesso hídrico independente da quantidade de palha de aveia na superfície. Em ano com pequeno déficit hídrico os tratamentos com mais palha produziram > 5% de massa seca da parte aérea e > 4% de grãos de soja, independente da compactação. O crescimento e o rendimento de grãos de soja em terras baixas oscilam de acordo com as condições hídricas, sendo influenciada pela compactação e quantidade de palha superficial.

Weed management in glyphosate-resistant maize / Manejo de plantas daninhas em milho resistente ao glifosato

The application of glyphosate associated with other herbicides is an important alternative for weed control in maize, to increase control spectrum and to minimize problems with resistance and tolerance from some species to the product. The objective of this work was to evaluate the efficacy, selectivity and effects on the grain yield components of glyphosate-resistant maize as a function of its application, associated or not with other pre- and postemergence herbicides. The design used was randomized blocks with four replications. Treatments consisted in the use of glyphosate combined with the herbicides: atrazine, [atrazine + simazine], [atrazine + oil], [atrazine + S-metolachlor], applied pre- and/or postemergence and [nicosulfuron + mesotrione] only postemergence, plus two controls, one weeded and one infested. The evaluated variables were maize phytotoxicity, weed control, ear insertion height, number of rows per ear, number of grains per row, one thousand grain mass and grain yield. Herbicide treatments caused low phytotoxicity to maize, less than 6%; control greater than 88, 95 and 95% for alexandergrass, turnip and sunflower, respectively, and did not affect grain yield components. The tested herbicides are selective to the hybrid Forseed 2A521 PW and effective in weed control. Weed control with weeding or herbicide increased maize Forseed 2A521 PW yield by 43%. The association of glyphosate with pre- or postemergence herbicides increased maize grain yield by approximately 14%.(AU)

O uso de glifosato associado com outros herbicidas torna-se uma alternativa importante para o manejo de plantas daninhas infestantes do milho, pois ele aumenta o espectro de controle, minimiza problemas com resistência e tolerância de plantas daninhas ao herbicida. O objetivo deste trabalho foi estudar a eficiência, a seletividade e os efeitos nos componentes de rendimento de grãos do milho resistente ao glifosato pelo uso dessa substância associada ou não a outros herbicidas aplicados em pré- e pós-emergência. O experimento foi instalado em delineamento de blocos ao acaso, com quatros repetições. Os tratamentos consistiram na utilização do glifosato em combinação com os herbicidas: atrazina, [atrazina + simazina], [atrazina + óleo], [atrazina + S-metolacloro], aplicados em pré- e/ou pós-emergência e o [nicosulfuron + mesotriona] somente em pós-emergência, além das testemunhas capinada e infestada. Avaliaram-se as variáveis: fitotoxicidade ao milho, controle de plantas daninhas, altura de inserção de espigas, número de fileiras e de grãos por espiga, peso de mil grãos e a produtividade de grãos. Os herbicidas ocasionaram baixa fitotoxicidade ao milho, inferior a 6%, controle superior a 88, 95 e 95% para papuã, nabo e girassol, respectivamente, e não influenciaram negativamente nos componentes relacionados ao rendimento de grãos da cultura. Os herbicidas testados são seletivos ao híbrido Forseed 2A521 PW e efetivos no controle de papuã, nabo e girassol. O manejo das plantas daninhas com capina ou herbicidas proporcionou aumento de cerca de 43% na produtividade de grãos do híbrido de milho Forseed 2A521 PW. O uso de glifosato em mistura de tanque com herbicidas aplicados em pré- ou pós-emergência incrementou, aproximadamente, 14% a produtividade de grãos de milho.(AU)

New perspectives for weeds control using autochthonous fungi with selective bioherbicide potential.

The prospection of bioherbicides has been an alternative to weed control, aiming at mitigating chemical risks to human, animal and environmental health due to extreme use of synthetic herbicides. In the present study, various fungi were isolated from plants with symptoms of fungal diseases for bioherbicide purposes against weeds (Urochloa plantaginea, Euphorbia heterophylla and Bidens pilosa). Fungi isolated were identified by molecular methods and enzymatic products obtained by fungi fermentation (cellulase, lipase, peroxidase, and amylase) were quantified. Bioherbicide selectivity study was performed on crops (soybean and corn), as well as on resistant weeds. Among the isolated fungi, Fusarium oxysporum, Fusarium ploriferatum, and Trichoderma koningiopsis presented bioherbicide potential. T. koningiopsis, in particular, presented the highest effect on Euphorbia heterophylla (popular name - Mexican fire plant), causing up to 60% of foliar damage, without presenting phytotoxicity against corn crop. New perspectives for weeds control and their use in corn crops were prospected, considering the bioherbicide selectivity described in this study.

Physicochemical characterization of fruits of Campomanesia guazumifolia (Cambess.) O. Berg (Myrtaceae)

Belonging to the family Myrtaceae, ‘sete capotes’ is a plant native to the Atlantic Forest biome. It produces yellow-green fruits between February and May. Although used for consumption by local populations, studies of the fruit are rare. The present work aimed at characterize the fruit in terms of physicochemical properties. We quantified mass, volume, moisture, pulp yield, pH, titratable acidity, soluble solids/titratable acidity, proteins, lipids, total sugars, reducing sugars, ash, macronutrients, micronutrients, pectin, vitamin C and phenolic compounds. Compared with commercial fruits and other native fruit trees, sete capotes is unusual for its pulp yield, vitamin C levels, total phenolic compound content, protein levels, calcium content, potassium levels and iron content. Through the results it is verified of the fruit as a functional food, mainly because of its nutritive content and because of its bioactive compounds. Our data emphasize the importance of study of the constituents of native flora.

Competitive interaction and economic injury level of Urochloa plantaginea in corn hybrids / Interação competitiva e nível de dano econômico de papuã em híbridos de milho

Identifying the competitive ability of corn hybrids and the economic injury level (EIL) caused by Urochloa plantaginea is important for the adoption of integrated management of this weed. This work aims to evaluate the interference and EIL of U. plantaginea infesting corn hybrids. Treatments included the corn hybrids Syngenta Status VIP3, Syngenta SX8394 VIP3, Pioneer P1630H, Pioneer 30F53 YH, and Dow 135021, as well as 12 densities of U. plantaginea for each hybrid. We used a rectangular hyperbolic model to describe the relationship between corn grain productivity loss and the explanatory variables ­ plant density (PD), dry mass (DM) of the aerial parts, ground cover (GC), and leaf area (LA). EIL was determined using grain productivity, control cost, corn price, and herbicide efficacy. The rectangular hyperbolic model provides a satisfactory estimate of grain productivity loss regarding the interference of U. plantaginea in corn culture. The hybrids Pioneer 30F53 YH and Pioneer P1630H presented better competitiveness and higher EIL. Sowing the hybrids Pioneer P1630H, Pioneer 30F53 YH, and Dow 135021 increased EIL, justifying the adoption of control measures for U. plantaginea at higher densities.(AU)

A identificação da habilidade competitiva de híbridos de milho e do nível de dano econômico (NDE) ocasionado pelo papuã (Urochloa plantaginea) torna-se importante para a adoção do manejo integrado dessa planta daninha. Diante disso, objetivou-se com este trabalho avaliar a interferência e o NDE de papuã infestante de híbridos de milho. Os tratamentos foram compostos pelos híbridos de milho Syngenta Status VIP 3, Syngenta SX8394 VIP 3, Pioneer P1630H, Pioneer 30F53 YH e Dow 135021 e 12 densidades de plantas de papuã para cada híbrido. Para descrever a relação entre a perda de produtividade de grãos do milho e as variáveis explicativas densidade de plantas (DP), massa seca da parte aérea (MS), cobertura do solo (CS) e área foliar (AF), usou-se o modelo da hipérbole retangular. A determinação do NDE foi efetuada usando-se a produtividade de grãos, custo de controle, preço do milho e eficiência do herbicida. O modelo da hipérbole retangular estima de modo satisfatório as perdas de produtividade de grãos em função da interferência do papuã na cultura do milho. Os híbridos Pionner 30F53 YH e Pionner P1630H apresentaram melhor competitividade e os maiores NDEs. A semeadura dos híbridos Pioneer P1630H, Pioneer 30F53 YH e Dow 135021 aumenta o NDE, justificando a adoção de medidas de controle em densidades mais elevadas.(AU)

Effect of herbicides in the oxidative stress in crop winter species.

Most herbicides applied in crop field, stay in the soil for a period, affecting next crop or even the plants using as green manure. Nowadays, the use of herbicides grow to increase productivity, mainly in the grain producing region north of Rio Grande do Sul state. The objective of this study was to evaluate the effects of herbicides fomesafen and sulfentrazone on antioxidant system in Avena sativa1, Vicia sativa2, Raphanus sativus and Lupinus albus. The plants were exposed to varying concentrations of fomesafen3 (0, 0.125, 0.25 and 0.5 kg ha -1) and sulfentrazone (0, 0.3, 0.6 and 1.2 kg ha-1). For this, the activities of, ascorbat peroxidase, catalase and guaiacol enzymes were analyzed, and the levels of MDA were quantificated. Fomesafen and sulfentrazone promoted alterations in balance of plants generating oxidative stress and elicited the response of the antioxidant system of plants, mainly in the high doses of fomesafen, for the species V. sativa and R. sativus. At the same time, the 1,2 kg ha -1 dose of sulfentrazone generated lipid peroxidation for V. sativa, R. sativus and L. albus. Additionally, A. sativa was the species that demonstrated low alterations on antioxidant system with the exposure to herbicide fomesafen and sulfentrazone. Thus, we can we can suggest that the species present a better response in defense of the oxidative stress generated by the herbicides.

Acute and chronic toxicity of pesticides on tadpoles of Physalaemus cuvieri (Anura, Leptodactylidae).

Brazil is the largest consumer of pesticides in the world. However, knowledge on how these pesticides affect wildlife is scarce. Among the vertebrates, amphibians are particularly important in research to assess the impact of pesticides because of the correlation between pesticide and the decline of these species. This study aimed to evaluate the acute and chronic toxicity of commercial formulations of pesticides, i.e., atrazine (herbicide), cypermethrin (insecticide), and tebuconazole (fungicide) in Physalaemus cuvieri tadpoles. Eggs were collected in nature and cultivated under controlled conditions in the laboratory. Toxicity tests were carried out under standard conditions to determine the lethal concentration (LC50) after 96 h of exposure and to determine the effect of sublethal concentrations after 7 days. In addition, we performed swimming activity tests on tadpoles exposed to sublethal concentrations. The lethal concentration (LC50) was 19.69 mg/L for atrazine, 0.24 mg/L for cypermethrin and 0.98 mg/L for tebuconazole. In the acute test, atrazine showed lower toxicity than cypermethrin and tebuconazole for P. cuvieri. Swimming activity was affected at sublethal doses of atrazine and cypermethrin, but was not after exposure to tebuconazole. Cypermethrin was the insecticide that most altered the swimming activity of the individuals tested. The risk evaluation analysis indicated risks for tadpoles exposed to three tested pesticides, specially cypermethrin.

Chemical control of California arrowhead (Sagittaria montevidensis) resistant to acetolactate synthase and photosystem II inhibiting herbicides in irrigated rice / Manejo químico de sagitária (Sagittaria montevidensis) resistente aos herbicidas inibidores de Acetolactato Sintase e Fotossistema II aplicados em arroz irrigado

ABSTRACT: California arrowhead is one of the primary weeds infesting paddy rice fields in the Brazilian states of Santa Catarina and Rio Grande do Sul, where the system of pre-germinated seeding is used. The objective of this study was to evaluate the selectivity and effectiveness of saflufenacil application in irrigated rice, either singly or in combination with other herbicides in the same application or sequentially, for the control of Sagittaria montevidensis biotype that is resistant to ALSand PSII-inhibiting herbicides. In the first experiment carried out in a greenhouse, saflufenacil was applied, either singly or in combination with penoxsulam, bispyribac-sodium, pyrazosulfuron-ethyl, bentazon, or propanil to the S. montevidensis (SAGMO 32) biotype and the irrigated rice variety Epagri 108. In the second experiment, single or combined (including sequential) applications of saflufenacil, bentazon, and cyhalofop-butyl were applied to Epagri 108 in open field conditions. Saflufenacil combined with propanil showed a high degree of phytotoxicity and a reduction in the accumulation of dry mass in Epagri 108. Application of saflufenacil, bentazon, and cyhalofop-butyl in combination or sequentially resulted in an increase in phytotoxicity in Epagri 108 compared to when applied singly. A pplication of saflufenacil singly or in combination with penoxsulam, bispyribac-sodium, bentazon, or pyrazosulfuron-ethyl did not adequately control SAGMO 32.

RESUMO: A sagitária tem sido considerada uma das principais plantas daninhas infestante das áreas orizícolas de Santa Catarina e do Rio Grande do Sul que utilizam o sistema de semeadura pré-germinado. Objetivou-se com o trabalho avaliar a seletividade e a eficácia da aplicação de saflufenacil em isolado, em mistura e/ou de forma sequencial com outros herbicidas em arroz irrigado, para o manejo de biótipos de Sagittaria montevidensis resistente aos herbicidas inibidores de ALS e FSII. O primeiro experimento, realizado em casa de vegetação, foi composto de aplicações de saflufenacil, de modo isolado ou combinado com penoxsulam, bispyribac-sodium, pyrazosulfuron-ethyl, bentazon ou propanil sobre o biótipo de S. montevidensis (SAGMO 32) e da cultivar de arroz irrigado Epagri 108. No segundo experimento, avaliaram-se, em condições de campo, aplicações isoladas, combinadas e sequencial de saflufenacil, bentazon e cyhalofop-butyl sobre a cultivar Epagri 108. O saflufenacil combinado com propanil apresenta elevada fitotoxicidade e redução do acúmulo de massa seca da cultivar Epagri 108. A aplicação de forma combinada ou sequencial de saflufenacil, bentazon e cyhalofop-butyl ocasiona aumento da fitotoxicidade à cultivar Epagri 108 em comparação àaplicação isolada. A aplicação isolada de saflufenacil ou combinada com penoxsulam, bispyribac-sodium, bentazon ou pyrazosulfuron-ethyl não proporcionou controle adequado do SAGMO 32.

Características fisiológicas de feijoeiro em competição com plantas daninhas / Physiological characteristics of beans in competition with weed

A competição das plantas daninhas com as culturas reduz o fornecimento de alguns recursos para as espécies, ocasionando deficiências que culminam em alterações nas características fisiológicas relacionadas à fotossíntese, como a deficiência hídrica e nutricional e a baixa qualidade ou quantidade de luminosidade. Assim, objetivou-se com o presente trabalho avaliar o efeito da competição e do manejo de plantas daninhas nas características fisiológicas de plantas de feijoeiro. Utilizou-se delineamento em blocos ao acaso com quatro repetições. Os tratamentos foram: plantas de feijoeiro submetidas à aplicação da mistura de herbicidas fomesafen+fluazifop-p-butyl (Robust®), cultivadas isoladamente e em competição com capim-marmelada (Brachiaria plantaginea), caruru (Amaranthus hybridus) e picão-preto (Bidens pilosa). As plantas de picão-preto foram as que mais afetam as características fisiológicas do feijoeiro, influenciando negativamente o consumo de CO2, a transpiração, a condutância estomática e principalmente a taxa fotossintética. Estes resultados podem estar relacionados ao fato dessa espécie daninha ser altamente eficiente no uso da água. A mistura de herbicidas fomesafen+fluazifop-p-butyl reduziu a taxa fotossintética do feijoeiro.

The weed competition with crops leads to lower supply of some resources for the species, causing deficiencies that culminate in changes in physiological characteristics related to photosynthesis, such as water deficiency and low nutritional quality or quantity of light. So aim with this study was to evaluate the effect of weed competition and management on physiological characteristics of bean plants. The experimental design comprised randomized blocks with four replications. The treatments were: bean plants subjected to the application of the herbicide mixture fomesafen + fluazifop-p-butyl (Robust ®), grown in isolation, in competition with alexandergrass (Brachiaria plantaginea), pigweed (Amaranthus hybridus) and beggar-ticks (Bidens pilosa). The plants are beggartick that most affect the physiological characteristics of bean, negatively influencing the consumption of CO2, transpiration, stomatal conductance and photosynthetic rate mainly for these results may be related to this photo weed be highly efficient water use. The herbicides mixture fomesafen + fluazifop-p-butyl reduced the photosynthetic rate of the bean plants.

Época de emergência de azevém e nabo sobre a habilidade competitiva da cultura da cevada / Time of emergency of ryegrass and wild radish on the competitive ability of barley crop

Objetivou-se com o trabalho avaliar os efeitos de épocas de emergência das plantas daninhas, azevém e nabo, sobre a cultura da cevada. O experimento foi conduzido em delineamento de blocos casualizados, arranjado em esquema fatorial 2x5, com três repetições. No fator A, foram alocadas as plantas competidoras (azevém e nabo) e no B as épocas de emergência (14 e 07 dias antes, no mesmo dia, 07 e 14 dias depois da emergência da cultura). No início da floração da cevada, foram mensuradas as variáveis: estatura das plantas, número de colmos ou caules, área foliar e matéria seca da parte aérea da cultura e das plantas daninhas. Todas as variáveis avaliadas da cevada apresentaram aumento de valor com o atraso da emergência das plantas daninhas. As espécies, cultivada ou daninhas, que emergiram antes, apresentaram maior habilidade competitiva, dominando o ambiente quando emergiram 14 dias antes uma da outra. Quanto mais tarde ocorrer a emergência das plantas daninhas com relação à cultura, menor é a interferência e, desse modo, maior é a habilidade da cevada em competir com a espécie daninha pelos recursos disponíveis no meio.

The objective was to evaluate the effects of times of emergence of the weed ryegrass species and wild radish on barley. The experiment was conducted in a randomized block design, arranged in a 2x5 factorial design, with three replicates. In factor A were allocated the competing species (ryegrass and wild radish) and in factor B the emergence periods (14 and 7 days before, on the same day, 7 and 14 days after crop emergence). At the beginning of barley flowering, the following variables were quantified: plant height, number of stalks or stems, leaf area, and shoot dry mass of crop and weeds. All barley variables had increased in value with the delay in weed emergence. The species, cultivated or weeds, that emerged earlier showed greater competitive ability, dominating the environment when emerging 14 days before each other. The later the emergence of weeds with respect to the crop, the less the interference they cause, and, thereby, the crop presents a greater ability to compete with the weeds for available resources in the environment.