ABSTRACT
AIM: Evaluation of composite formulation of yeast double stranded RNA with polyglucinum (dsRNA-PG) effect on non-specific antiviral resistance factors in mice in comparison with commercial formulation Ridostin. MATERIALS AND METHODS: dsRNA and Ridostin formulations were injected intramuscularly once at the dose of 5 mg/ml, polyglucinum--at the dose of 3.75 mg/ml. 3, 5, 24, 48 and 72 hours after the injection serum interferon levels, neutrophil oxidation-reduction activity parameters, peritoneal macrophage phagocyte activity levels were analyzed in mice blood samples. RESULTS: New dsRNA and polyglucinum containing composite formulation is a non-specific resistance system stimulator. dsRNA-PG effect on interferon synthesis and mice phagocyte activity was higher than with Ridostin and developed earlier. Neutrophil function activation by the formulation had a prolonged effect. A possible explanation for increased activity of dsRNA and polyglucinum composite formulation is a modulating effect by the polysaccharide component. CONCLUSION: The new formulation may have a more intensive and prolonged protective effect against influenza virus in comparison with Ridostin.
Subject(s)
Antiviral Agents/administration & dosage , Dextrans/administration & dosage , Interferon Inducers/administration & dosage , Interferons/blood , RNA, Double-Stranded/administration & dosage , Animals , Mice , Neutrophils/drug effects , Neutrophils/immunology , Orthomyxoviridae/drug effects , RNA, Fungal/administration & dosageABSTRACT
The effect of yeast double-stranded RNA (dsRNA) and of hyaluronic acid (HA) compositions produced on the interferon synthesis, peritoneal phagocytic activity of macrophages and on the hematological parameters were studied in an experiment with white noninbred mice. HA was shown to enhance the dsRNA-induced interferon synthesis, to inhibit the leukopenic reaction and to produce no effect on the phagocytosis-stimulating activity. The data obtained are indicative of that HA is a promising preparation regarding its use within the interferon-inducing compositions based on dsRNA.
Subject(s)
Hyaluronic Acid/analogs & derivatives , Immunity, Innate/drug effects , Interferons/biosynthesis , Macrophages, Peritoneal/drug effects , RNA, Double-Stranded/pharmacology , RNA, Fungal/pharmacology , Administration, Cutaneous , Animals , Dose-Response Relationship, Drug , Injections, Intraperitoneal , Interferons/blood , Leukocyte Count , Macrophages, Peritoneal/immunology , Male , Mice , Phagocytosis/drug effects , RNA, Double-Stranded/chemistry , RNA, Fungal/chemistryABSTRACT
Human liposomal recombinant alpha 2b-interferon topically applied to laboratory animals was tested for antiviral activity, pharmacokinetics, and toxicity. The interferon was shown to penetrate through the skin and to circulate in the blood of experimental animals longer than its injectable form, and to exhibit its antiviral activity against genital herpes in guinea pigs. It produced no toxic, skin-irritant, or allergic effects in laboratory animals.
Subject(s)
Antiviral Agents/pharmacology , Herpes Genitalis/drug therapy , Interferon-alpha/pharmacology , Administration, Cutaneous , Animals , Antiviral Agents/administration & dosage , Antiviral Agents/pharmacokinetics , Antiviral Agents/toxicity , Cells, Cultured/drug effects , Fibroblasts/drug effects , Guinea Pigs , Humans , Interferon alpha-2 , Interferon-alpha/administration & dosage , Interferon-alpha/pharmacokinetics , Interferon-alpha/toxicity , Liposomes , Male , Mice , Rats , Rats, Wistar , Recombinant Proteins , Skin AbsorptionABSTRACT
Toxic properties of recombinant human tumor necrosis factor (TNF-beta) were studied on noninbred albino mice. In the maximum tolerable doses the preparation induced a decrease in the body weight and temperature of the animals as well as development of glyco- and leukopenic reactions and damage of the internal organ structures. The preparation effects were observed early after the exposure and were mainly reversible. The most TNF-beta sensitive organs were the liver, lungs, adrenal gland, thymus and spleen. The major link in the pathological process development under the effect of the TNF-beta toxic doses was likely increased intravascular blood coagulability evident from a marked procoagulant activity of the preparation.
