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Nat Commun ; 14(1): 3962, 2023 07 05.
Article in English | MEDLINE | ID: mdl-37407555

ABSTRACT

Huntington's disease (HD) is a neurodegenerative disorder caused by CAG-repeat expansions in the huntingtin (HTT) gene. The resulting mutant HTT (mHTT) protein induces toxicity and cell death via multiple mechanisms and no effective therapy is available. Here, we employ a genome-wide screening in pluripotent mouse embryonic stem cells (ESCs) to identify suppressors of mHTT toxicity. Among the identified suppressors, linked to HD-associated processes, we focus on Metal response element binding transcription factor 1 (Mtf1). Forced expression of Mtf1 counteracts cell death and oxidative stress caused by mHTT in mouse ESCs and in human neuronal precursor cells. In zebrafish, Mtf1 reduces malformations and apoptosis induced by mHTT. In R6/2 mice, Mtf1 ablates motor defects and reduces mHTT aggregates and oxidative stress. Our screening strategy enables a quick in vitro identification of promising suppressor genes and their validation in vivo, and it can be applied to other monogenic diseases.


Subject(s)
Huntington Disease , Neurodegenerative Diseases , Mice , Animals , Humans , Disease Models, Animal , Zebrafish/genetics , Zebrafish/metabolism , Huntington Disease/metabolism , Neurons/metabolism , Neurodegenerative Diseases/metabolism , Huntingtin Protein/genetics , Huntingtin Protein/metabolism
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