ABSTRACT
Sixty-three samples of the more solid material (sludge) separated from the effluent plants of dairy factories were examined for the presence of salmonellas and brucellas. Salmonellas were isolated from two samples (S. heidelberg. [1]; S. indiana [1]. No brucellas were isolated. None of the samples supported the growth of S. dublin. Salmonellas added to effluent sludge at a concentration of 10(6) organisms/ml. survived less than 70 days. The sludge from dairy factory effluent plants does not appear to be a source for the spread of salmonellosis or brucellosis.
Subject(s)
Animal Diseases/etiology , Bacteria/isolation & purification , Dairying , Fertilizers , Industrial Waste , Animals , Brucella/isolation & purification , Hydrogen-Ion Concentration , Salmonella/growth & development , Salmonella/isolation & purification , TemperatureSubject(s)
Adenosine Triphosphatases/analysis , Cerebral Cortex/enzymology , Acetylcholine/analysis , Acetylcholinesterase/analysis , Animals , Biological Assay , Cattle , Centrifugation , Centrifugation, Density Gradient , Guinea Pigs , Microscopy, Electron , Phosphates/analysis , Proteins/analysis , RNA/analysis , Sodium/physiology , Species Specificity , Succinate Dehydrogenase/analysis , UltracentrifugationABSTRACT
1. The molecular weight of arginine kinase from lobster muscle has been determined by three procedures: ultracentrifuge analysis, gel filtration and density-gradient centrifugation. 2. The three methods give similar results and the best estimate of the molecular weight is 37000. 3. The enzyme does not readily show association-dissociation phenomena. 4. The usefulness of density-gradient centrifugation for determinations of molecular weight is briefly discussed.
