The Isolation and Structure Characterization of the Active Oligosaccharide DP-6 from Cultured Panax ginseng Cells.

The oligosaccharins isolated from acid-hydrolyzed cultured cells of Panax ginseng are a mixture of DP-3 to 12. The samples were passed through an active carbon column, a Dowex (H(+)) column, and a Bio-Gel P-2 column to obtain oligosaccharides DP-6, 7 and 8. They were finally separated by HPLC and the DP-6 oligosaccharide was obtained. Experiments showed that it could increase the growth rate of many cultured plant cells. Its structure was characterized by GC, GC-MS, FAB(-)-MS and (13)C-NMR measurements.