Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 64
Filter
Add more filters










Publication year range
1.
Pflugers Arch ; 469(5-6): 779-795, 2017 06.
Article in English | MEDLINE | ID: mdl-28176016

ABSTRACT

Choline is present at cholinergic synapses as a product of acetylcholine degradation. In addition, it is considered a selective agonist for α5 and α7 nicotinic acetylcholine receptors (nAChRs). In this study, we determined how choline affects action potentials and excitatory synaptic transmission using extracellular and intracellular recording techniques in CA1 area of hippocampal slices obtained from both mice and rats. Choline caused a reversible depression of evoked field excitatory postsynaptic potentials (fEPSPs) in a concentration-dependent manner that was not affected by α7 nAChR antagonists. Moreover, this choline-induced effect was not mimicked by either selective agonists or allosteric modulators of α7 nAChRs. Additionally, this choline-mediated effect was not prevented by either selective antagonists of GABA receptors or hemicholinium, a choline uptake inhibitor. The paired pulse facilitation paradigm, which detects whether a substance affects presynaptic release of glutamate, was not modified by choline. On the other hand, choline induced a robust increase of population spike evoked by orthodromic stimulation but did not modify that evoked by antidromic stimulation. We also found that choline impaired recurrent inhibition recorded in the pyramidal cell layer through a mechanism independent of α7 nAChR activation. These choline-mediated effects on fEPSP and population spike observed in rat slices were completely reproduced in slices obtained from α7 nAChR knockout mice, which reinforces our conclusion that choline modulates synaptic transmission and neuronal excitability by a mechanism independent of nicotinic receptor activation.


Subject(s)
CA1 Region, Hippocampal/physiology , Choline/pharmacology , Cholinergic Agents/pharmacology , Excitatory Postsynaptic Potentials , Pyramidal Cells/physiology , Receptors, Nicotinic/metabolism , Animals , CA1 Region, Hippocampal/cytology , CA1 Region, Hippocampal/metabolism , GABA Antagonists/pharmacology , Hemicholinium 3/pharmacology , Male , Mice , Pyramidal Cells/drug effects , Pyramidal Cells/metabolism , Rats , Rats, Sprague-Dawley , Receptors, Nicotinic/genetics
2.
Bioresour Technol ; 102(15): 7280-8, 2011 Aug.
Article in English | MEDLINE | ID: mdl-21612917

ABSTRACT

The effect of fatty acids chain length (LC) and its interaction with unsaturation degree (UD) on important biodiesel quality parameters was studied. Low calorific value, kinematic viscosity, flash point, cetane number and cold filter plugging point of biodiesel blends covering a wide range of fatty acids were analyzed. Analytical results were processed with statistical regression to obtain a prediction model for each property, combining LC and UD. Due to the antagonistic effects of the chemical composition over quality properties, the Derringer desirability function was proposed to allow the most suitable fatty acid composition. This target was achieved considering an average of 1.26 double bounds and 17 carbon atoms. A set of combinations of LC and UD values that provides a biodiesel that fits the European standard EN 14214 was proposed. It was found that a reduction of FAME LC allows a lower UD while keeping biodiesel specifications under the standard limits.


Subject(s)
Biofuels/analysis , Fatty Acids/analysis , Physical Phenomena , Plant Oils/chemistry , Biomechanical Phenomena , Cold Temperature , Esters/analysis , Filtration , Models, Chemical , Surface Properties , Viscosity
3.
Bioresour Technol ; 102(2): 1044-50, 2011 Jan.
Article in English | MEDLINE | ID: mdl-20801017

ABSTRACT

Presence of unreacted glycerides in biodiesel may reduce drastically its quality. This is why conversion of raw material in biodiesel through transesterification needs to readjust reaction parameter values to complete. In the present work, monitoring of glycerides transformation in biodiesel during the transesterification of vegetable oils was carried out. To check the influence of the chemical composition on glycerides conversion, selected vegetable oils covered a wide range of fatty acid composition. Reactions were carried out under alkali-transesterification in the presence of methanol. In addition, a multiple regression model was proposed. Results showed that kinetics depends on chemical and physical properties of the oils. It was found that the optimal reaction temperature depends on both length and unsaturation degree of vegetable oils fatty acid chains. Vegetable oils with higher degree of unsaturation exhibit faster monoglycerides conversion to biodiesel. It can be concluded that fatty acid composition influences reaction parameters and glycerides conversion, hence biodiesel yield and economic viability.


Subject(s)
Biofuels/analysis , Fatty Acids/analysis , Fatty Acids/chemistry , Glycerides/chemistry , Plant Oils/chemistry , Temperature , Analysis of Variance , Esterification , Models, Chemical , Regression Analysis , Time Factors
4.
Acta Physiol (Oxf) ; 194(2): 97-109, 2008 Oct.
Article in English | MEDLINE | ID: mdl-18485124

ABSTRACT

AIMS: This study looks into the physiology of the exocytosis of catecholamines released by adrenal medullary chromaffin cells. We have comparatively explored the exocytotic responses elicited by two different patterns of depolarizing stimulation: the widely employed square depolarizing pulses (DPs) and trains of acetylcholine-like action potentials (APs), likely the physiological mode of stimulation in the intact innervated adrenal medulla. APs were applied at 30 Hz, a frequency similar to that produced in a stressful situation. METHODS: Patch-clamp, cell membrane capacitance, single cell amperometry and fluorescence were the techniques used. The variations of calcium entry measured as the integral of the calcium current, cytosolic calcium (measured with the calcium-sensitive fluorescent probe fluo-4) and exo-endocytosis (membrane capacitance variations) were the parameters measured. RESULTS: Trains of AP depolarizations produced distinct responses compared to those of square depolarizations: (1) Calcium current amplitude decreased to a lesser extent along the AP train; (2) calcium entry and capacitance increments raised linearly with stimulation time whereas they deviated from linearity when square depolarizations were used; (3) slower activation and faster delayed decay phase of cytosolic calcium transients; (4) capacitance increments varied linearly with calcium entry with APs and deviated from linearity with longer depolarizations; (5) little endocytosis after stimulation with longer trains of APs and pronounced endocytosis with longer square depolarizations. CONCLUSIONS: Stimulation of chromaffin cells with trains of APs produced patterns of cytosolic calcium transients, exocytotic and endocytotic responses quite different from those elicited by the widely employed DPs. Our study is relevant from the methodological and physiological points of view.


Subject(s)
Action Potentials/physiology , Calcium/metabolism , Chromaffin Cells/metabolism , Cytosol/metabolism , Membrane Potentials/physiology , Acetylcholine/pharmacology , Action Potentials/drug effects , Adrenal Glands/cytology , Adrenal Glands/metabolism , Animals , Cattle , Cell Polarity/physiology , Cells, Cultured , Cholinergic Agents/pharmacology , Chromaffin Cells/drug effects , Chromaffin Cells/physiology , Exocytosis/physiology , Patch-Clamp Techniques/methods
5.
Eur J Neurosci ; 27(5): 1097-110, 2008 Mar.
Article in English | MEDLINE | ID: mdl-18312591

ABSTRACT

The role of postsynaptic nicotinic receptors for acetylcholine (nAChRs) in mediating fast neurotransmission processes in the CNS is controversial. Here we have studied the modulation of synaptic transmission by an agonist (choline) and an allosteric modulator (5-OH-indole) of alpha7 nAChRs in rat hippocampal neuronal cultures. Choline evoked a fast inactivating inward current, causing neuron depolarization and action potential discharge, thereby enhancing the spontaneous postsynaptic current activity (sPSCs). This effect was markedly enhanced when both choline and 5-OH-indole were applied together and was blocked by the selective alpha7 nAChR antagonist methyllycaconitine. This choline action was suppressed by the GABA(A) receptor antagonist bicuculline, while the glutamatergic receptor antagonist kynurenic acid had no effect. Frequency, but not amplitude or area, of both excitatory and inhibitory miniature postsynaptic currents (mEPSCs and mIPSCs) were drastically reduced when Ca(2+) influx was blocked by Cd(2+). Additionally, nAChR activation did not modify the mIPSCs. These data suggest that Ca(2+) influx through the highly Ca(2+)-permeablealpha7 nAChRs was insufficient to directly activate neurotransmitter release, suggesting that a tight colocalization of this receptor with secretory hot spots is unlikely. In a few cases, the activation of alpha7 AChRs led to a suppression of spontaneous synaptic transmission. This effect may be related to the potentiation of GABAergic interneurons that inhibit the spontaneous activity of neurons making synapses with the cell under study. We suggest that GABA release is modulated by alpha7 nAChRs. Thus, selective allosteric modulators of alpha7 nAChRs could have potential therapeutic applications in brain disorders such as epilepsy and schizophrenia and in alterations of cognition and sensory processing.


Subject(s)
Hippocampus/physiology , Interneurons/physiology , Receptors, Nicotinic/physiology , Synaptic Transmission/physiology , gamma-Aminobutyric Acid/physiology , Allosteric Regulation/drug effects , Allosteric Regulation/physiology , Animals , Cells, Cultured , Female , GABA Antagonists/pharmacology , Hippocampus/drug effects , Interneurons/drug effects , Pregnancy , Rats , Rats, Sprague-Dawley , Synaptic Transmission/drug effects , alpha7 Nicotinic Acetylcholine Receptor
6.
Acta Physiol (Oxf) ; 192(2): 287-301, 2008 Feb.
Article in English | MEDLINE | ID: mdl-18005392

ABSTRACT

Here we review the tight neural control of the differential secretion into the circulation, of the adrenal medullary hormones adrenaline and noradrenaline. One or the other catecholamines are differentially released on various stress conditions. This is specifically controlled by central nervous system nuclei at the cortex, hypothalamus and spinal cord. Different firing patterns of splanchnic nerves and nicotinic or muscarinic receptors cause the selective release of noradrenaline or adrenaline, to adapt the body to the 'fight or flight' reaction, or during severe hypoglycaemia, haemorrhage, cold, acute myocardial infarction or other severe stressful conflicts. Endogenously acetylcholine (ACh) released at the splanchnic nerve-chromaffin cell synapse, acting on muscarinic and nicotinic receptors, causes membrane depolarization and action potentials (AP) in chromaffin cells. These changes vary with the animal species, the cell preparation (intact bisected adrenal, adrenal slices, or isolated fresh or cultured cells) or the recording technique (intracellular microelectrodes, patch-clamp, perforated-patch, cell-attached). Conflicting results leave many open questions concerning the actions of ACh on chromaffin cell excitability. The use of adrenal slices and field electrical stimulation will surely provide new insights into these mechanisms. Chromaffin cells have been thoroughly used as models to study the relationship between Ca2+ entry, cytosolic Ca2+ signals, exocytosis and endocytosis, using patch-clamp and amperometric techniques. Cells have been stimulated with single depolarizing pulses (DPs), DP trains and with simulated AP waveforms. These approaches have provided useful information but we have no data on APs generated by pulsatile secretory quanta of ACh, trying to mimic the intermittent and repetitive splanchnic nerve discharge of the neurotransmitter. We present some recent experiments using ultrashort ACh pulses (25 ms), that cause non-desensitizing repetitive APs with each ACh pulse, at low ACh concentrations (30 microM). Ultrashort pulses of a high ACh concentration (1000 microM) causes a single AP followed by a prolonged depolarization. It could be interesting trying to correlate these 'patterns of splanchnic nerve discharge' with Ca2+ signals and exocytosis. This, together with the use of adrenal slices and transmural electrical stimulation of splanchnic nerves will provide new physiologically sound data on the regulation of adrenal medullary secretion.


Subject(s)
Adrenal Medulla/physiology , Catecholamines/metabolism , Central Nervous System/metabolism , Peripheral Nervous System/metabolism , Acetylcholine/physiology , Action Potentials/physiology , Animals , Chromaffin Cells/metabolism , Humans , Models, Biological , Patch-Clamp Techniques , Receptors, Muscarinic , Splanchnic Nerves/physiology , Stress, Physiological/metabolism
7.
Neuroscience ; 145(3): 866-72, 2007 Mar 30.
Article in English | MEDLINE | ID: mdl-17291692

ABSTRACT

Although alpha7-receptors are considered the main target for neuroprotection, other receptor subtypes (alpha4beta2 or alpha3beta4) have also been implicated. Hence, we have used alpha7-transgenic mice, to study the hypothesis that alpha7-receptors play a dominant role in mediating neuroprotection in an in vitro model of ischemia. We have used rat and mouse hippocampal slices to establish the model of nicotinic neuroprotection against oxygen and glucose deprivation (OGD). Neuronal damage caused by OGD during 1 h plus 3 h re-oxygenation, was quantified by measuring lactate dehydrogenase (LDH) release from hippocampal slices. In rat hippocampal slices, OGD increased over twofold basal LDH release. Such increase was reduced when treated with 10-100 microM nicotine; maximal protection afforded by nicotine amounted to 46%. This neuroprotection was antagonized by the non-selective nicotinic receptor for acetylcholine (nAChR) blocker mecamylamine (10 microM). In hippocampal slices from wild-type control mice, nicotine (100 microM) decreased by 54.4% LDH release evoked by OGD plus re-oxygenation. In contrast, nicotine failed to exert neuroprotection in alpha7 knockout mice. This finding reinforces the view that the hippocampal neuroprotective effects of nicotine are predominantly linked to alpha7 receptors.


Subject(s)
Glucose/physiology , Nicotine/pharmacology , Oxygen/physiology , Receptors, Nicotinic/deficiency , Receptors, Nicotinic/genetics , Animals , Female , Hippocampus/pathology , L-Lactate Dehydrogenase/analysis , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Neurons/pathology , Oxidation-Reduction , Rats , Rats, Sprague-Dawley , alpha7 Nicotinic Acetylcholine Receptor
8.
J Mol Neurosci ; 30(1-2): 57-8, 2006.
Article in English | MEDLINE | ID: mdl-17192626

ABSTRACT

Depending on experimental conditions, cell model, and pattern and type of depolarizing stimuli, the relationship between calcium entry ([Ca2+]c) and the release of neurotransmitters and hormones varies from exponential (power of 3-4) to near linear (power of 1.5) or linear function. Here, we present a study using the more physiological stimulation pattern based on acetylcholine (ACh)-like action potentials, in voltage-clamped bovine chromaffin cells, with the perforated-patch configuration of the patch-clamp technique and 2 mM extracellular calcium. Trains of ACh-like action potentials or square depolarizing pulses of increasing length were applied, and calcium currents (ICa), total calcium entry (QCa), and exocytosis (DeltaCm) measured.


Subject(s)
Acetylcholine/physiology , Action Potentials/physiology , Calcium/physiology , Chromaffin Cells/physiology , Exocytosis/physiology , Adrenal Glands/physiology , Animals , Biological Transport , Cattle , Membrane Potentials/physiology
9.
Rev Neurol ; 42(8): 471-7, 2006.
Article in Spanish | MEDLINE | ID: mdl-16625509

ABSTRACT

INTRODUCTION: Among the numerous pathophysiological theories that attempt to explain the development of Alzheimer's disease (AD) there are two facts that stand out above the rest: on the one hand, the formation of neurofibrillary tangles inside cells and, on the other, the extra-cellular deposition of beta-amyloid protein. These two mechanisms lead to neurodegeneration and the death of cells by means of a process called 'apoptosis' or 'programmed cell death'. In the early stages of this neurodegenerative process it is more pronounced in cholinergic-type brain centres. This led to the formulation of the so-called cholinergic theory of Alzheimer, which provides the rationale behind the use of the drugs that are currently available to treat this disease, namely, acetylcholine esterase (AChE) inhibitors (rivastigmine, donepezil and galanthamine). DEVELOPMENT AND CONCLUSIONS: We review the possible pharmacological approaches that could help to prevent or delay cell death, and which act on the mechanisms involved in the production of neurofibrillary tangles or the deposition of beta-amyloid protein. We also review the main characteristics of cholinergic neurotransmission, which will help us to understand the therapeutic approaches that have been applied in an attempt to enhance deficient cholinergic neurotransmission. One of the most notable of these is the amount of attention recently being paid to the enzyme AChE, which increases the bioavailability of the neurotransmitter in the cholinergic synapses by preventing the hydrolysis of acetylcholine; these are the only drugs currently available for the symptomatic treatment of this disease.


Subject(s)
Alzheimer Disease/drug therapy , Alzheimer Disease/physiopathology , Cholinesterase Inhibitors/therapeutic use , Acetylcholine/metabolism , Acetylcholinesterase/metabolism , Alzheimer Disease/pathology , Amyloid beta-Peptides/metabolism , Apoptosis/physiology , Humans , Neurofibrillary Tangles/metabolism , Synaptic Transmission/physiology
10.
Rev. neurol. (Ed. impr.) ; 42(8): 471-477, 16 abr., 2006. ilus
Article in Es | IBECS | ID: ibc-045445

ABSTRACT

Introducción. Entre las múltiples teorías fisiopatológicas que intentan explicar el desarrollo de la enfermedad de Alzheimer(EA) destacan dos hechos: por un lado, la formación de ovillos neurofibrilares en el interior celular y, por otro, el depósito extracelular de la proteína â-amiloide. Estos dos mecanismos conducen a la neurodegeneración y a la muerte celular a través de un mecanismo denominado apoptosis o 'muerte celular programada'. Este proceso neurodegenerativo comienza siendo más patente en centros cerebrales de tipo colinérgico, lo que llevó a postular la llamada teoría colinérgica de la EA, en la que se basa el uso de los fármacos actualmente disponibles para el tratamiento de esta enfermedad, los inhibidores de la acetilcolina esterasa (AChE) (rivastigmina,donepecilo y galantamina). Desarrollo y conclusiones. Se revisan los posibles abordajes farmacológicos que podrían ayudara prevenir o retrasar la muerte celular, los cuales actúan sobre los mecanismos de producción de los ovillos neurofibrilares o el depósito de la proteína â-amiloide. Se revisan igualmente las principales características de la neurotransmisión colinérgica, lo que ayudará a entender las aproximaciones terapéuticas que se han aplicado para tratar de incrementar esta neurotransmisión colinérgicadeficitaria, entre las que destaca la atención centrada recientemente en la inhibición de la enzima AChE que, al prevenir la hidrólisis de la acetilcolina, aumenta la biodisponibilidad del neurotransmisor en las sinapsis colinérgicas; éstos son los únicos fármacos disponibles actualmente para el tratamiento sintomático dela EA (AU)


Introduction. Among the numerous pathophysiological theories that attempt to explain the development of Alzheimer’s disease (AD) there are two facts that stand out above the rest: on the one hand, the formation of neurofibrillary tangles inside cells and, on the other, the extra-cellular deposition of â-amyloid protein. These two mechanisms lead to neurodegeneration and the death of cells by means of a process called ‘apoptosis’ or ‘programmed cell death’. In the early stages of this neurodegenerative process it is more pronounced in cholinergic-type brain centres. This led to the formulation of the so-called cholinergic theory of Alzheimer, which provides the rationale behind the use of the drugs that are currently available to treat this disease, namely, acetylcholine esterase (AChE) inhibitors (rivastigmine, donepezil and galanthamine).Development and conclusions. We review the possible pharmacological approaches that could help to prevent or delay cell death, and which act on the mechanisms involved in the production of neurofibrillary tangles or the deposition of b-amyloid protein. We also review the main characteristics of cholinergic neurotransmission, which will help us to understand the therapeutic approaches that have been applied in an attempt to enhance deficient cholinergic neurotransmission. One of the most notable of these is the amount of attention recently being paid to the enzyme AChE, which increases the bioavailability of the neurotransmitter in the cholinergic synapses by preventing the hydrolysis of acetylcholine; these are the only drugs currently available for the symptomatic treatment of this disease (AU)


Subject(s)
Humans , Cholinesterase Inhibitors/pharmacokinetics , Alzheimer Disease/drug therapy , Galantamine/therapeutic use , Acetylcholinesterase/physiology
11.
J Neurosci ; 21(8): 2553-60, 2001 Apr 15.
Article in English | MEDLINE | ID: mdl-11306608

ABSTRACT

The hypothesis that the buffering of Ca(2+) by mitochondria could affect the Ca(2+)-dependent inhibition of voltage-activated Ca(2+) channels, (I(Ca)), was tested in voltage-clamped bovine adrenal chromaffin cells. The protonophore carbonyl cyanide m-chlorophenyl-hydrazone (CCCP), the blocker of the Ca(2+) uniporter ruthenium red (RR), and a combination of oligomycin plus rotenone were used to interfere with mitochondrial Ca(2+) buffering. In cells dialyzed with an EGTA-free solution, peak I(Ca) generated by 20 msec pulses to 0 or +10 mV, applied at 15 sec intervals, from a holding potential of -80 mV, decayed rapidly after superfusion of cells with 2 microm CCCP (tau = 16.7 +/- 3 sec; n = 8). In cells dialyzed with 14 mm EGTA, CCCP did not provoke I(Ca) loss. Cell dialysis with 4 microm ruthenium red or cell superfusion with oligomycin (3 microm) plus rotenone (4 microm) also accelerated the decay of I(Ca). After treatment with CCCP, decay of N- and P/Q-type Ca(2+) channel currents occurred faster than that of L-type Ca(2+) channel currents. These data are compatible with the idea that the elevation of the bulk cytosolic Ca(2+) concentration, [Ca(2+)](c), causes the inhibition of L- and N- as well as P/Q-type Ca(2+) channels expressed by bovine chromaffin cells. This [Ca(2+)](c) signal appears to be tightly regulated by rapid Ca(2+) uptake into mitochondria. Thus, it is plausible that mitochondria might efficiently regulate the activity of L, N, and P/Q Ca(2+) channels under physiological stimulation conditions of the cell.


Subject(s)
Calcium Channels/metabolism , Calcium/metabolism , Chromaffin Cells/metabolism , Mitochondria/metabolism , Animals , Calcium/pharmacology , Calcium Channel Blockers/pharmacology , Calcium Channels, L-Type/drug effects , Calcium Channels, L-Type/metabolism , Calcium Channels, N-Type/drug effects , Calcium Channels, N-Type/metabolism , Calcium Channels, P-Type/drug effects , Calcium Channels, P-Type/metabolism , Calcium Channels, Q-Type/drug effects , Calcium Channels, Q-Type/metabolism , Carbonyl Cyanide m-Chlorophenyl Hydrazone/analogs & derivatives , Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology , Cattle , Cells, Cultured , Chelating Agents/pharmacology , Chromaffin Cells/cytology , Chromaffin Cells/drug effects , Intracellular Fluid/metabolism , Ionophores/pharmacology , Mitochondria/drug effects , Oligomycins/pharmacology , Patch-Clamp Techniques , Receptors, Nicotinic/metabolism , Rotenone/pharmacology , Ruthenium Red/pharmacology , Sodium Channels/metabolism
12.
Eur J Pharmacol ; 417(1-2): 99-109, 2001 Apr 06.
Article in English | MEDLINE | ID: mdl-11301064

ABSTRACT

The blockade of exocytosis induced by the putative endogenous ligand for imidazoline receptors, agmatine, was studied by using on-line measurement of catecholamine release in bovine adrenal medullary chromaffin cells. Agmatine inhibited the acetylcholine-evoked release of catecholamines in a concentration-dependent manner (IC(50)=366 microM); the K(+)-evoked release of catecholamines was unaffected. Clonidine (100 microM) and moxonidine (100 microM) also inhibited by 75% and 50%, respectively, the acetylcholine-evoked response. In cells voltage-clamped at -80 mV, the intermittent application of acetylcholine pulses elicited whole-cell inward currents (I(ACh)) that were blocked 63% by 1 mM agmatine. The onset of blockade was very fast (tau(on) = 31 ms); the recovery of the current after washout of agmatine also occurred very rapidly (tau(off = 39 ms). Efaroxan (10 microM) did not affect the inhibition of I(ACh) elicited by 1 mM agmatine. I(ACh) was blocked 90% by 100 microM clonidine and 50% by 100 microM moxonidine. The concentration-response curve for acetylcholine to elicit inward currents was shifted to the right in a non-parallel manner by 300 microM agmatine. The blockade of I(ACh) caused by agmatine (100 microM) was similar at various holding potentials, around 50%. When intracellularly applied, agmatine did not block I(ACh). At 1 mM, agmatine blocked I(Na) by 23%, I(Ba) by 14%, I(K(Ca)) by 16%, and I(K(VD)) by 18%. In conclusion, agmatine blocks exocytosis in chromaffin cells by blocking nicotinic acetylcholine receptor currents. In contrast to previous views, these effects seem to be unrelated to imidazoline receptors.


Subject(s)
Agmatine/pharmacology , Catecholamines/metabolism , Chromaffin Cells/drug effects , Receptors, Drug/physiology , Acetylcholine/pharmacology , Agmatine/metabolism , Animals , Benzofurans/pharmacology , Binding Sites , Binding, Competitive , Calcium Channels/drug effects , Calcium Channels/physiology , Cattle , Cells, Cultured , Chromaffin Cells/metabolism , Chromaffin Cells/physiology , Clonidine/pharmacology , Dose-Response Relationship, Drug , Electric Stimulation , Imidazoles/pharmacology , Imidazoline Receptors , Membrane Potentials/drug effects , Potassium/pharmacology , Potassium Channels/drug effects , Potassium Channels/physiology , Receptors, Nicotinic/drug effects , Receptors, Nicotinic/metabolism , Receptors, Nicotinic/physiology , Sodium Channels/drug effects , Sodium Channels/physiology
13.
Pflugers Arch ; 440(2): 253-63, 2000 Jun.
Article in English | MEDLINE | ID: mdl-10898526

ABSTRACT

We established primary cultures of human pheochromocytoma chromaffin cells. We then tried to find what mechanism of their secretory apparatus could be altered to produce the massive release of catecholamines into the circulation and the subsequent hypertensive crisis observed in patients suffering this type of tumor. Their whole-cell Ca2+ channel currents could be pharmacologically separated into components similar to those found in normal human adrenal chromaffin cells: 20% L-type, 30% N-type, and 50% P/Q-type Ca2+ channels. However, modulation of the channels by exogenous or endogenous ATP and opioids, via a G-protein membrane-delimited pathway, was deeply altered; some cells having no modulation or very little modulation alternated with others having normal modulation. This may be the cause of the uncontrolled secretory response, measured amperometrically at the single-cell level. Some cells secreted for long time periods and were insensitive to nifedipine (L-type channel blocker) or to omega-conotoxin MVIIC (N/P/Q-type channel blocker), while others were highly sensitive to nifedipine and partially sensitive to omega-conotoxin MVIIC. Alteration of the autocrine/paracrine modulation of Ca2+ channels may lead to indiscriminate Ca2+ entry and exacerbate catecholamine release responses in human pheochromocytoma cells.


Subject(s)
Adrenal Gland Neoplasms/physiopathology , Calcium Channels/metabolism , Chromaffin Cells/physiology , Exocytosis/physiology , Pheochromocytoma/physiopathology , Adenosine Triphosphate/pharmacology , Adrenal Gland Neoplasms/pathology , Adult , Barium/physiology , Calcium Channels/physiology , Chromaffin Cells/metabolism , Dopamine beta-Hydroxylase/metabolism , Electric Conductivity , Enkephalin, Ala(2)-MePhe(4)-Gly(5)-/pharmacology , Enkephalin, D-Penicillamine (2,5)-/pharmacology , Epinephrine/blood , Humans , Immunohistochemistry , Male , Middle Aged , Norepinephrine/blood , Phenylethanolamine N-Methyltransferase/metabolism , Pheochromocytoma/pathology , Tumor Cells, Cultured
14.
Eur J Neurosci ; 11(10): 3574-84, 1999 Oct.
Article in English | MEDLINE | ID: mdl-10564365

ABSTRACT

The inhibition of L-type channels induced by either bath application of ATP, opioids and catecholamines or by endogenously released neurotransmitters was investigated in rat chromaffin cells with whole-cell recordings (5 mM Ba2+). In both cases, the L-type current, isolated pharmacologically using omega-toxin peptides and potentiated by Bay K 8644, was inhibited by approximately 50% with nearly no changes to the activation-inactivation kinetics. Inhibition was voltage independent at a wide range of potentials (-20 to +50 mV) and insensitive to depolarizing prepulses (+100 mV, 50 ms). Onset and offset of the inhibition were fast (time constants: tau(on) approximately 0.9 s, tau(off) approximately 3.6 s), indicating a rapid mechanism of channel modulation. Whether induced exogenously or from the released granules content in conditions of stopped cell superfusion, the neurotransmitter action was reversible and largely prevented by either intracellular GDP-beta-S, cell treatment with pertussis toxin or simultaneous application of P2y,2x delta/mu-opioidergic and alpha/beta-adrenergic antagonists. This suggests the existence of converging modulatory pathways by which autoreceptors-activated G-proteins reduce the activity of L-type channels through fast interactions. The autocrine inhibition of L-type currents, which was absent in superfused isolated cells, was effective on cell clusters, suggesting that L-type channels may be potently inhibited by cell exocytosis under physiological conditions resembling the intact adrenal glands.


Subject(s)
Adenosine Triphosphate/pharmacology , Analgesics, Opioid/pharmacology , Calcium Channels, L-Type/physiology , Chromaffin Cells/physiology , Enkephalin, D-Penicillamine (2,5)-/pharmacology , Epinephrine/pharmacology , Sympathomimetics/pharmacology , 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester/pharmacology , Adrenal Medulla/cytology , Animals , Autocrine Communication/drug effects , Autocrine Communication/physiology , Barium/pharmacokinetics , Calcium Channel Agonists/pharmacology , Calcium Channel Blockers/pharmacology , Cells, Cultured , Chromaffin Cells/cytology , Chromaffin Cells/drug effects , Electric Stimulation , Enkephalin, Ala(2)-MePhe(4)-Gly(5)-/pharmacology , Female , GTP-Binding Proteins/physiology , Guanosine Diphosphate/analogs & derivatives , Guanosine Diphosphate/pharmacology , Ion Channel Gating/drug effects , Ion Channel Gating/physiology , Nifedipine/pharmacology , Norepinephrine/pharmacology , Patch-Clamp Techniques , Pertussis Toxin , Rats , Rats, Sprague-Dawley , Receptors, Adrenergic/physiology , Receptors, Purinergic/physiology , Thionucleotides/pharmacology , Virulence Factors, Bordetella/pharmacology
15.
J Physiol ; 516 ( Pt 2): 421-32, 1999 Apr 15.
Article in English | MEDLINE | ID: mdl-10087342

ABSTRACT

1. In this study we pose the question of why the bovine adrenal medullary chromaffin cell needs various subtypes (L, N, P, Q) of the neuronal high-voltage activated Ca2+ channels to control a given physiological function, i.e. the exocytotic release of catecholamines. One plausible hypothesis is that Ca2+ channel subtypes undergo different patterns of inactivation during cell depolarization. 2. The net Ca2+ uptake (measured using 45Ca2+) into hyperpolarized cells (bathed in a nominally Ca2+-free solution containing 1.2 mM K+) after application of a Ca2+ pulse (5 s exposure to 100 mM K+ and 2 mM Ca2+), amounted to 0.65 +/- 0.02 fmol cell-1; in depolarized cells (bathed in nominally Ca2+-free solution containing 100 mM K+) the net Ca2+ uptake was 0.16 +/- 0.01 fmol cell-1. 3. This was paralleled by a dramatic reduction of the increase in the cytosolic Ca2+ concentration, [Ca2+]i, caused by Ca2+ pulses applied to fura-2-loaded single cells, from 1181 +/- 104 nM in hyperpolarized cells to 115 +/- 9 nM in depolarized cells. 4. A similar decrease was observed when studying catecholamine release. Secretion was decreased when K+ concentration was increased from 1.2 to 100 mM; the Ca2+ pulse caused, when comparing the extreme conditions, the secretion of 807 +/- 35 nA of catecholamines in hyperpolarized cells and 220 +/- 19 nA in depolarized cells. 5. The inactivation by depolarization of Ca2+ entry and secretion occluded the blocking effects of combined omega-conotoxin GVIA (1 microM) and omega-agatoxin IVA (2 microM), thus suggesting that depolarization caused a selective inactivation of the N- and P/Q-type Ca2+ channels. 6. This was strengthened by two additional findings: (i) nifedipine (3 microM), an L-type Ca2+ channel blocker, suppressed the fraction of Ca2+ entry (24 %) and secretion (27 %) left unblocked by depolarization; (ii) FPL64176 (3 microM), an L-type Ca2+ channel 'activator', dramatically enhanced the entry of Ca2+ and the secretory response in depolarized cells. 7. In voltage-clamped cells, switching the holding potential from -80 to -40 mV promoted the loss of 80 % of the whole-cell inward Ca2+ channel current carried by 10 mM Ba2+ (IBa). The residual current was blocked by 80 % upon addition of 3 microM nifedipine and dramatically enhanced by 3 microM FPL64176. 8. Thus, it seems that the N- and P/Q-subtypes of calcium channels are more prone to inactivation at depolarizing voltages than the L-subtype. We propose that this different inactivation might occur physiologically during different patterns of action potential firing, triggered by endogenously released acetylcholine under various stressful conditions.


Subject(s)
Calcium Channels, N-Type , Calcium Channels/metabolism , Calcium/metabolism , Chromaffin Cells/metabolism , Adrenal Medulla/cytology , Adrenal Medulla/drug effects , Adrenal Medulla/metabolism , Animals , Calcium Channel Agonists/pharmacology , Calcium Channel Blockers/pharmacology , Calcium Channels/drug effects , Calcium Channels, L-Type , Calcium Signaling/physiology , Catecholamines/metabolism , Cattle , Cells, Cultured , Chromaffin Cells/drug effects , Electrophysiology , Fluorescent Dyes , Fura-2 , Ion Channel Gating/physiology , Patch-Clamp Techniques
16.
Pflugers Arch ; 437(1): 104-13, 1998 Dec.
Article in English | MEDLINE | ID: mdl-9817794

ABSTRACT

Applying 10-s pulses of 10 mM Ba2+ to resting or K+-depolarized (70 mM) bovine adrenal chromaffin cells superfused with a nominal 0Ca2+ solution produced a large catecholamine secretory peak. In contrast, pulses of 10 mM Sr2+ or Ca2+ did not induce secretion from polarized resting cells, and induced smaller and narrower secretory peaks from depolarized cells; the areas of the secretory peaks from depolarized cells were 1.87, 3.06 and 27.4 nA s, respectively, for Ca2+, Sr2+ and Ba2+. Ca2+ channel currents in isolated cells or in cells surrounded by other unpatched cells (cell cluster) were studied with either the continuous-flow or the flow-stop method. When applied to an isolated cell, flow-stop reduced the amplitude of ICa by 19%, ISr by 31%, and IBa by 53%, compared with the current amplitude measured under continuous-flow conditions. This decrease in current amplitude was accompanied by a pronounced slowing down of current activation and could be largely relieved by applying strong depolarizing prepulses (facilitation). Under continuous-flow conditions, 10 microM exogenous ATP reduced (about 50%) ICa, ISr and IBa similarly. On the other hand, the use of Na+ as a charge carrier through Ca2+ channels, or intracellular dialysis with 1 mM BAPTA prevented the modulation of current by flow-stop. In cell clusters, activating secretion from unpatched cells, by either 10 mM Ba2+, 100 microM acetylcholine or 70 mM K+, caused a pronounced slowing down of current activation, as well as a decrease of its magnitude in the voltage-clamped cell immersed in the cluster. Such modulation of isolated cells was not observed. These data are compatible with the idea that the secretory activity of adrenal medullary chromaffin cells "in situ" controls the activity of their Ca2+ channels through autocrine/paracrine mechanisms.


Subject(s)
Adrenal Medulla/cytology , Calcium Channels/physiology , Chromaffin Cells/physiology , Acetylcholine/pharmacology , Adenosine Triphosphate/pharmacology , Animals , Barium/pharmacology , Calcium Channels/drug effects , Catecholamines/metabolism , Cattle , Chelating Agents/pharmacology , Chromaffin Cells/drug effects , Egtazic Acid/analogs & derivatives , Egtazic Acid/pharmacology , Electric Conductivity , Potassium/pharmacology , Sodium/metabolism , Strontium/pharmacology
17.
Proc Natl Acad Sci U S A ; 95(24): 14184-9, 1998 Nov 24.
Article in English | MEDLINE | ID: mdl-9826675

ABSTRACT

Methyllycaconitine (MLA), alpha-conotoxin ImI, and alpha-bungarotoxin inhibited the release of catecholamines triggered by brief pulses of acetylcholine (ACh) (100 microM, 5 s) applied to fast-superfused bovine adrenal chromaffin cells, with IC50s of 100 nM for MLA and 300 nM for alpha-conotoxin ImI and alpha-bungarotoxin. MLA (100 nM), alpha-conotoxin ImI (1 microM), and alpha-bungarotoxin (1 microM) halved the entry of 45Ca2+ stimulated by 5-s pulses of 300 microM ACh applied to incubated cells. These supramaximal concentrations of alpha7 nicotinic receptor blockers depressed by 30% (MLA), 25% (alpha-bungarotoxin), and 50% (alpha-conotoxin ImI) the inward current generated by 1-s pulses of 100 microM ACh, applied to voltage-clamped chromaffin cells. In Xenopus oocytes expressing rat brain alpha7 neuronal nicotinic receptor for acetylcholine nAChR, the current generated by 1-s pulses of ACh was blocked by MLA, alpha-conotoxin ImI, and alpha-bungarotoxin with IC50s of 0.1 nM, 100 nM, and 1.6 nM, respectively; the current through alpha3 beta4 nAChR was unaffected by alpha-conotoxin ImI and alpha-bungarotoxin, and weakly blocked by MLA (IC50 = 1 microM). The functions of controlling the electrical activity, the entry of Ca2+, and the ensuing exocytotic response of chromaffin cells were until now exclusively attributed to alpha3 beta4 nAChR; the present results constitute the first evidence to support a prominent role of alpha7 nAChR in controlling such functions, specially under the more physiological conditions used here to stimulate chromaffin cells with brief pulses of ACh.


Subject(s)
Acetylcholine/pharmacology , Adrenal Medulla/physiology , Cholinergic Agents/pharmacology , Chromaffin Cells/physiology , Conotoxins , Nicotinic Antagonists/pharmacology , Receptors, Nicotinic/physiology , Aconitine/analogs & derivatives , Aconitine/pharmacology , Adrenal Medulla/cytology , Adrenal Medulla/drug effects , Animals , Brain/metabolism , Bungarotoxins/pharmacology , Calcium/metabolism , Cattle , Chromaffin Cells/cytology , Chromaffin Cells/drug effects , Female , Membrane Potentials/drug effects , Membrane Potentials/physiology , Mollusk Venoms/pharmacology , Oligopeptides/pharmacology , Oocytes/drug effects , Oocytes/physiology , Rats , Receptors, Nicotinic/drug effects , Receptors, Nicotinic/genetics , Xenopus laevis , alpha7 Nicotinic Acetylcholine Receptor
18.
Br J Pharmacol ; 124(6): 1187-96, 1998 Jul.
Article in English | MEDLINE | ID: mdl-9720790

ABSTRACT

1. Incubation of bovine adrenal chromaffin cells with veratridine (10-100 microM) during 24 h, caused a concentration-dependent release of the cytosolic lactate dehydrogenase (LDH) into the bathing medium, an indicator of cell death. Lubeluzole or its R(-) enantiomer, R91154, did not enhance LDH release. Both lubeluzole and R91154 (0.3-10 microM) decreased the veratridine-induced LDH release. 2. Penfluridol did not increase LDH release at concentrations 0.003-1 microM; 3-10 microM increased LDH release to 50-60%, after 24 h exposure. Penfluridol (0.03-0.3 microM) did not protect against the cytotoxic effects of veratridine; at 1 microM, 15% protection was produced. Higher concentrations (3-10 microM) enhanced the cytotoxic effects of veratridine. 3. Ba2+ ions caused a concentration-dependent increase of LDH release. This cytotoxic effect was partially prevented by 3 microM lubeluzole and fully counteracted by 1 microM penfluridol. R91154 was less potent than lubeluzole and only protected against the lesion induced by 0.5 mM Ba2+. 4. Ouabain (10 microM during 24 h) increased LDH release to about 30%. Both lubeluzole (0.3-10 microM) and the lower concentrations of penfluridol (0.003-0.3 microM) prevented the ouabain cytotoxic effects. At higher concentrations (3 microM), penfluridol increased drastically the ouabain cytotoxic effects. 5. 6-Hydroxydopamine (6-OHDA) caused significant cytotoxic effects at 30 and 100 microM. Lubeluzole (3-10 microM) or penfluridol (0.03-0.3 microM) had no cytoprotective effects against 6-OHDA. 6. Lubeluzole (3 microM), R91154 (3 microM) and penfluridol (1 microM) blocked the current through Na+ channels in voltage-clamped chromaffin cells (I(Na)) by around 20-30%. Ca2+ current through Ca2+ channels (I(Ca)) was inhibited 57% by lubeluzole and R91154 and 50% by penfluridol. The effects of penfluridol were not washed out, but those of lubeluzole and R91154 were readily reversible. 7. Lubeluzole (3 microM) induced reversible blockade of the oscillations of the cytosolic Ca2+, [Ca2+]i, in fura-2-loaded cells exposed to 30 or 100 microM veratridine. Penfluridol (1 microM) inhibited those oscillations in an irreversible manner. 8. The results suggest that lubeluzole and its R-isomer caused cytoprotection against veratridine cell damage, by blocking the veratridine stimulated Na+ and Ca2+ entry, as well as the [Ca2+]i oscillations. The Ba2+ and ouabain cytotoxic effects were prevented more efficiently by penfluridol, likely by blocking the plasmalemmal Na+/Ca2+ exchanger. It remains dubious whether these findings are relevant to the reported neuroprotective action of lubeluzole in stroke; the doubt rests in the stereoselective protecting effects of lubeluzole in in vivo stroke models, as opposed to its lack of stereoselectivity in the in vitro model reported here.


Subject(s)
Barium/pharmacology , Chromaffin Cells/drug effects , Ouabain/pharmacology , Oxidopamine/pharmacology , Piperidines/pharmacology , Thiazoles/pharmacology , Veratridine/pharmacology , Adrenal Glands/cytology , Adrenal Glands/drug effects , Adrenal Glands/metabolism , Animals , Calcium/metabolism , Cattle , Chromaffin Cells/metabolism , Cytosol/metabolism , Neuroprotective Agents/pharmacology , Penfluridol/pharmacology , Sodium/metabolism
19.
Pflugers Arch ; 436(5): 696-704, 1998 Oct.
Article in English | MEDLINE | ID: mdl-9716702

ABSTRACT

Human adrenal medullary chromaffin cells were prepared and cultured from a cystic tumoral adrenal gland whose medullary tissue was unaffected. Adrenaline-containing and noradrenaline-containing cells were identified using a confocal fluorescence microscope and antibodies against dopamine beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT). Current/voltage (I/V) curves performed with the voltage-clamped cells bathed in 10 mM Ba2+ (holding potential, Vh=-80 mV) revealed the presence of only high-threshold voltage-dependent Ca2+ channels; T-type Ca2+ channels were not seen. By using supramaximal concentrations of selective Ca2+ channel blockers, the whole-cell IBa could be fractionated into various subcomponents. Thus, IBa had a 25% fraction sensitive to 1 microM nifedipine (L-type channels), 21% sensitive to 1 microM omega-conotoxin GVIA (N-type channels), and 60% sensitive to 2 microM omega-agatoxin IVA (P/Q-type channels). The activation of IBa was considerably slowed down, and the peak current was inhibited upon superfusion with 10 microM ATP. The slow activation and peak current blockade were reversed by strong depolarizing pre-pulses to +100 mV (facilitation). A drastic facilitation of IBa was also observed in voltage-clamped human chromaffin cell surrounded by other unclamped cells; in contrast, in voltage-clamped cells not immersed in a cell cluster, facilitation was scarce. So, facilitation of Ca2+ channels in a voltage-clamped cell seems to depend upon the exocytotic activity of neighbouring unclamped cells, which is markedly increased by Ba2+. It is concluded that human adrenal chromaffin cells mostly express P/Q-types of voltage-dependent Ca2+ channels (60%). L-Type channels and N-type channels are also expressed, but to a considerably minor extent (around 20% each). This dominance of P/Q-type channels in human chromaffin cells clearly contrasts with the relative proportion of each channel type expressed by chromaffin cells of five other animal species studied previously, where the P/Q-type channels accounted for 5-50%. The results also provide strong support for the hypothesis that Ca2+ channels of human chromaffin cells are regulated in an autocrine/paracrine fashion by materials co-secreted with the catecholamines, i.e. ATP and opiates.


Subject(s)
Calcium Channels, N-Type , Calcium Channels/physiology , Chromaffin Cells/physiology , Adenosine Triphosphate/pharmacology , Adrenal Gland Neoplasms/pathology , Adrenal Glands/cytology , Adult , Animals , Barium/pharmacology , Calcium Channel Blockers/pharmacology , Calcium Channels/drug effects , Calcium Channels/metabolism , Cattle , Cell Count , Cell Separation , Cells, Cultured , Chromaffin Cells/cytology , Cysts , Dopamine beta-Hydroxylase/analysis , Enkephalin, Methionine/pharmacology , Female , Humans , Immunohistochemistry , Phenylethanolamine N-Methyltransferase/analysis
20.
Am J Physiol ; 274(6): C1745-54, 1998 06.
Article in English | MEDLINE | ID: mdl-9611141

ABSTRACT

Toxin-gamma (Tgamma) from the Brazilian scorpion Tityus serrulatus venom caused a concentration- and time-dependent increase in the release of norepinephrine and epinephrine from bovine adrenal medullary chromaffin cells. Tgamma was approximately 200-fold more potent than veratridine judged from EC50 values, although the maximal secretory efficacy of veratridine was 10-fold greater than that of Tgamma (1.2 vs. 12 microg/ml of catecholamine release). The combination of both toxins produced a synergistic effect that was particularly drastic at 5 mM extracellular Ca2+ concentration ([Ca2+]o), when 30 microM veratridine plus 0.45 microM Tgamma were used. Tgamma (0.45 microM) doubled the basal uptake of 45Ca2+, whereas veratridine (100 microM) tripled it. Again, a drastic synergism in enhancing Ca2+ entry was seen when Tgamma and veratridine were combined; this was particularly pronounced at 5 mM [Ca2+]o. Veratridine induced oscillations of cytosolic Ca2+ concentration ([Ca2+]i) in single fura 2-loaded cells without elevation of basal levels. In contrast, Tgamma elevated basal [Ca2+]i levels, causing only small oscillations. When added together, Tgamma and veratridine elevated the basal levels of [Ca2+]i without causing large oscillations. Tgamma shifted the current-voltage (I-V) curve for Na+ channel current to the left. The combination of Tgamma with veratridine increased the shift of the I-V curve to the left, resulting in a greater recruitment of Na+ channels at more hyperpolarizing potentials. This led to enhanced and more rapid accumulation of Na+ in the cell, causing cell depolarization, the opening of voltage-dependent Ca2+ channels, and Ca2+ entry and secretion.


Subject(s)
Adrenal Medulla/physiology , Chromaffin System/physiology , Neurotoxins/pharmacology , Scorpion Venoms/pharmacology , Veratridine/pharmacology , Adrenal Medulla/drug effects , Animals , Calcium/metabolism , Calcium/pharmacology , Calcium Channel Blockers/pharmacology , Calcium Channels/physiology , Calcium Radioisotopes , Cattle , Chromaffin System/drug effects , Drug Synergism , Electric Conductivity , Kinetics , Norepinephrine/metabolism , Sodium Channels/physiology
SELECTION OF CITATIONS
SEARCH DETAIL
...